Detection and analysis of triterpenic compounds in apple extracts

Butkevičiūtė, Aurita; Liaudanskas, Mindaugas; Kviklys, D.; Zymonė, Kristina; Raudonis, Raimondas; Viškelis, Pranas; Uselis, N.; Janulis, Valdimaras

doi:10.1080/10942912.2018.1506478

Cited by 26 publications

(29 citation statements)

References 39 publications

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“…Subsequently, these frozen samples were lyophilized with a ZIRBUS sublimator 3 × 4 × 5/20 (ZIRBUS technology, Bad Grund, Germany) at a pressure of 0.01 mbar (condenser temperature: -85°C). The lyophilized samples were ground to fine powder by using a Retsch 200 mill electric grinder (Haan, Germany) [1]. Loss on drying before the analysis was determined by drying the apple lyophilisate in a laboratory drying oven to complete the evaporation of water and volatile compounds (temperature: 105°C; the difference in weight between measurements: up to 0.01 g) and by calculating the difference in raw material weight before and after drying [35].…”

Section: Preparation Of Samplesmentioning

confidence: 99%

“…The conditions of the extraction were chosen based on the results of the tests for setting the extraction conditions. The obtained extract was filtered through a paper filter, and the residue on the filter was washed with acetone in a 10 mL flask until the exact volume was reached [1].…”

Section: Extractionmentioning

confidence: 99%

“…For the quantitative analysis, the calibration curve was obtained by injecting the known concentrations of different standard compounds. All the identified triterpenic compounds were quantified at 205 nm wavelength [1].…”

Section: Instrumentation and Chromatographic Conditionsmentioning

confidence: 99%

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Seasonal variation of the qualitative and quantitative composition of triterpenic compounds and antioxidant activity in vitro in the apple extracts of cultivars grown in Lithuania

Butkevičiūtė¹,

Urbštaitė²,

Liaudanskas³

et al. 2020

chemija

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Apple (Malus domestica Borkh.) fruits are rich in phenolic glycosides, triterpenic acids and other biologically active compounds. The apples are widely used as food products due to their biologically active compounds that have specific biological effects. It is important to use high quality apples or their recycling products, so it is necessary to investigate the qualitative and quantitative composition of the bioactive compounds. The amount of triterpenic acids varies during different phenological stages of apple development and maturation. In order to determine in which phenological stage of apple growth the quantity of triterpenic acids was highest, high-performance liquid chromatography was applied. The highest total amounts of triterpenic compounds were detected at the beginning of the phenological stage of fruit development. The study showed that the highest amounts of triterpenic acids were detected at the beginning of the phenological stage of apple development, while as the apple matured, the amount of triterpenic acids decreased from 2.63 ± 0.26 mg/g to 1.6 ± 0.28 mg/g. In this study, we identified and quantified four triterpenic compounds, which by the quantitative composition of triterpenic acids could be arranged in the following ascending order: betulinic acid < corosolic acid < oleanolic acid < ursolic acid. In order to use herbal extracts for medical practice it is important to perform biological effects study in vitro and in vivo. Antioxidants with different mechanisms of action neutralize harmful reactive oxygen and nitrogen forms and enhance antioxidants protection systems. The antioxidant activity of apple extracts in vitro varied during different phenological stages of the fruit. The strongest antiradical and reductive activities were observed at the beginning of apple development. In order to determine the relationship between the antioxidant activity of the acetone extracts of apples assessed by DPPH, ABTS, CUPRAC and FRAP assays and the total amount of triterpenic acids in these extracts, a correlation analysis was carried out. The strongest positive correlation was observed between the amount of oleanolic acid and the antioxidant activity assessed by DPPH, ABTS and CUPRAC methods (respectively, r = 0.778, r = 0.784 and r = 0.720).

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Section: Preparation Of Samplesmentioning

confidence: 99%

Section: Extractionmentioning

confidence: 99%

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Seasonal variation of the qualitative and quantitative composition of triterpenic compounds and antioxidant activity in vitro in the apple extracts of cultivars grown in Lithuania

Butkevičiūtė¹,

Urbštaitė²,

Liaudanskas³

et al. 2020

chemija

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“…Quantitative evaluation of triterpenes was determined by applying the technique described by. [34] During the analysis, 1 g of lyophilized powder of quince leaves (exact weight) was weighed, added to 10 mL of acetone (100%, v/v), and extracted in a Sonorex Digital ultrasonic bath 10 P (Bandelin Electronic GmbH & Co. KG, Berlin, Germany) for 10 min at 60°C. The ultrasound strength was 1100 W, and frequency -80 kHz.…”

Section: Quantitative Evaluation Of Triterpenesmentioning

confidence: 99%

Japanese Quince Chaenomeles Japonica (Thunb.) Lindl. ex Spach Leaves a New Source of Antioxidants for Food

Urbanavičiūtė

Liaudanskas

Segliņa

et al. 2019

International Journal of Food Properties

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Japanese quince (Chaenomeles japonica (Thunb.)) is a very important ornamental plant that is rich in many biochemical compounds such as antioxidants and secondary metabolites that have positive effects on human health. The aim of the study was to evaluate the biochemical compounds in the leaves of three Japanese quince (Chaenomeles japonica (Thunb.)) Lindl. ex Spach cultivars (ʻRasaʼ, ʻDariusʼ and ʻRondoʼ). The results showed that the leaves of Japanese quince have strong antiradical activity (1091 ± 22-1135 ± 15 µmol TE/g) in the CUPRAC reaction system. The analysis by the HPLC method revealed that the major polyphenol group in Japanese quince cultivars leaves was phenolic acids and the most common polyphenol compound is chlorogenic acid (11.2-52.4 mg/g DW). The most common triterpene is ursolic acid (3.7-6.8 mg/g DW). Japanese quince leaf powder can be used as a food or beverage additive and enriches our diet with compounds with strong antioxidant activity. ARTICLE HISTORY

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“…Despite the importance of these properties, triterpene compounds in apples are still under-researched. Our previous studies quantified the four triterpenic compounds -corosolic, betulinic, oleonolic and ursolic acids -which are the most common triterpenes found in apple peel (Butkevičiūtė et al 2018.…”

Section: Introductionmentioning

confidence: 99%

Location effects across northeastern Europe on bioactive compounds in apple fruit

Viškelis

Uselis

Liaudanskas

et al. 2019

AFSci

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A multi-location trial was performed in apple orchards planted in 2005 under a uniform scheme in Poland, Lithuania, Latvia and Estonia. The aim of this study was to evaluate the impact of the geographical apple-tree growing location on the accumulation of dominant bioactive compounds in apple fruits: phenolics and triterpenes as the most valuable substances for human health. The study included two apple cultivars, ‘Auksis’ and ‘Ligol’, on B.396 rootstock and was carried out from 2016 to 2018. Geographical apple-tree growing location had a significant impact on the composition and total content of the tested bioactive compounds in apple fruits. Increased accumulation of phenolic and triterpene compounds was recorded in the south–north direction. This could be explained by the different climate conditions in the trial locations: the sum of active temperatures gradually decreased by 200–300 °C in the south–north direction, and the vegetation period becomes shorter. Apples grown in Estonia, depending on the tested cultivar, had 77–139% more total phenolic compounds. Significant differences were recorded for all groups of phenolic compounds. The differences between the trial sites in accumulation of triterpene compounds were lower, although apples in at farthest north location had 18–32% more total triterpene compounds than apples grown in south.

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Detection and analysis of triterpenic compounds in apple extracts

Cited by 26 publications

References 39 publications

Seasonal variation of the qualitative and quantitative composition of triterpenic compounds and antioxidant activity in vitro in the apple extracts of cultivars grown in Lithuania

Seasonal variation of the qualitative and quantitative composition of triterpenic compounds and antioxidant activity in vitro in the apple extracts of cultivars grown in Lithuania

Japanese Quince Chaenomeles Japonica (Thunb.) Lindl. ex Spach Leaves a New Source of Antioxidants for Food

Location effects across northeastern Europe on bioactive compounds in apple fruit

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