Detection and Analysis of a Glucose 6-Phosphate Dehydrogenase Phenotype B Cell Line Contamination2

Lelbovitz, A; Wright, William C.; Pathak, Sen; Siciliano, Michael J.; Daniels, Walter P.

doi:10.1093/jnci/63.3.635

Cited by 22 publications

(5 citation statements)

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“…Before leaving this section, we should emphasize that these results are another manifestation of one the more dismaying and insidious problems besetting cancer cell biology research: cross contamination of tumor cell lines (50)(51)(52). We do not know the source of the mix-up between MDAY sarcoma and the presumptive thymoma cells which were sent to us from Stockholm.…”

Section: Origin and Properties Of Mday-d2: A Highly Metastatic Mouse mentioning

confidence: 99%

Tumor progression in metastasis: an experimental approach using lectin resistant tumor variants

et al. 1982

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A novel model of tumor progression in metastatic cancer is described which grew out of attempts to derive stable non-metastatic variants from a highly metastatic mouse tumor called MDAY-D2. The variants were obtained by selection of so-called lectin-resistant (LecR) membrane mutants using toxic concentrations of wheat germ agglutinin (WGA) as the selective agent, after mutagenesis. Cloned WGAR variants almost all appeared to be highly tumorigenic and metastatic, but displayed altered growth properties which were highly suggestive of major cellular phenotypic alterations occurring prior to metastasis. This were confirmed with the discovery that spontaneous visceral metastases always consisted of WGA-sensitive (WGAS) 'revertant' tumor cells. Such revertants also arose at the site of the subcutaneous inoculation and, with time, comprised an increasing proportion of the tumor cells at that location. The WGAS/high metastatic phenotype was stable in vitro or in vivo, implying the WGAR leads to WGAS shift had an underlying genetic basis. Thus, it appeared that the WGAR tumor cells could not metastasize, because of either an intrinsic cellular defect or a host imposed barrier, but that this block could be circumvented through a genetic change in the WGAR tumor cells which was accompanied by reversion of the WGAR phenotype. Non-tumorigenic (tum-) WGAR variants were also obtained, but in these cases the mutagenesis treatment itself appeared responsible for development of the tum- phenotype. The reduced tumorigenicity had an underlying immunological basis, a finding which could be exploited to immunotherapeutically treat established visceral metastases of poorly immunogenic tumors. Throughout these studies, emphasis was placed on the considerable potential of using tumor cell populations having various stable drug-resistant genetic markers to monitor aspects of tumorigenesis, tumor progression, and metastasis.

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Section: Origin and Properties Of Mday-d2: A Highly Metastatic Mouse mentioning

confidence: 99%

Tumor progression in metastasis: an experimental approach using lectin resistant tumor variants

et al. 1982

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“…As mentioned above, we identified SPA-sensitive CRC cell lines HT29 and SW480 and SPA-insensitive CRC cell lines SW620 and RKO (Figure D,F). Interestingly, the insensitive CRC cell line SW620 was isolated from the tissue of a 51-year-old Caucasian man (blood group A, Rh+), as was the sensitive CRC cell line SW480 . To reveal the antiproliferative mechanisms of SPA and LM2I, we identified differentially expressed genes (DEGs) between the sensitive CRC cell lines, HT29 and SW480, and insensitive CRC cell lines, which were SW620 and RKO.…”

Section: Resultsmentioning

confidence: 99%

Spinosyn A and Its Derivative Inhibit Colorectal Cancer Cell Growth via the EGFR Pathway

Peng,

Zou,

et al. 2023

J. Nat. Prod.

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Spinosyn A (SPA), derived from a soil microorganism, Saccharopolyspora spinosa, and its derivative, LM2I, has potential inhibitory effects on a variety of cancer cells. However, the effects of SPA and LM2I in inhibiting the growth of human colorectal cancer cells and the molecular mechanisms underlying these effects are not fully understood. Cell viability was tested by using a 3-(4,5-dimethylthiazol-2yl-)-2,5-diphenyltetrazolium bromide (MTT) assay and a colony formation assay. On the basis of the IC 50 values of SPA and LM2I in seven colorectal cancer (CRC) cell lines, sensitive (HT29 and SW480) and insensitive (SW620 and RKO) cell lines were screened. The GSE2509 and GSE10843 data sets were used to identify 69 differentially expressed genes (DEGs) between sensitive and insensitive cell lines. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, and protein−protein interactions (PPI) were performed to elucidate the molecular mechanisms of the DEGs. The hub gene of the DEGs was detected by Western blot analysis and verified using the CRISPR/Cas9 system. Our data indicate that SPA and its derivative LM2I have significant antiproliferative activity in seven colorectal cancer cell lines and colorectal xenograft tumors. On the basis of bioinformatics analysis, it was demonstrated that epidermal growth factor receptor (EGFR) was the hub gene of the DEGs and was associated with the inhibitory effects of SPA and LM2I in CRC cell lines. The study also revealed that SPA and LM2I inhibited the EGFR pathway in vitro and in vivo.

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“…Particular interest arose from contamination by SW 408, another human tumor cell line (Leibovitz et al, 1979;Nelson-Rees, 1979;Nelson-Rees et al, 1981). Examples of discrepancies were presented between early and late passage cultures (HS 852 cell line) due to contamination, and proved that six tumor cell lines were found identical to each other, probably because of a common contaminant.…”

Section: Prior Experience With Cell Cross-contamination: Evolution Ofmentioning

confidence: 99%

Cell cross-contamination in cell cultures: The silent and neglected danger

Marković

Marković²

1998

In Vitro Cell.Dev.Biol.-Animal

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Cell cross-contamination in cell cultures is a common problem during cell culturing and use. Contamination invalidates research results, compromises the comparison of results between laboratories, reduces reproducibility required in industrial production of cell lines, and may lead to unusable therapeutic products. The problem can be solved by increasing the awareness of its seriousness and by introducing regular quality control of cell cross-contamination in every laboratory where cells are grown and used.

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Detection and Analysis of a Glucose 6-Phosphate Dehydrogenase Phenotype B Cell Line Contamination2

Cited by 22 publications

References 0 publications

Tumor progression in metastasis: an experimental approach using lectin resistant tumor variants

Tumor progression in metastasis: an experimental approach using lectin resistant tumor variants

Spinosyn A and Its Derivative Inhibit Colorectal Cancer Cell Growth via the EGFR Pathway

Cell cross-contamination in cell cultures: The silent and neglected danger

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