Detection and activity evaluation of radical scavenging compounds by using DPPH free radical and on-line HPLC-DPPH methods

Bandonienė, Donata; Murkovic, Michaël; Pfannhauser, W.; Venskutonis, Petras Rimantas; Gruzdienė, D.

doi:10.1007/s00217-001-0430-9

Cited by 119 publications

(67 citation statements)

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“…Growth inhibition threshold is influenced by other environmental conditions as well [49]. Jatropha curcas is rich in phenolic compounds [50,51] and flavonoids [52]. Jatropha curcas contains polyphenolic compounds such as flavanols, cinnamic acids, coumarins and caffeic acid.…”

Section: Resultsmentioning

confidence: 99%

Influence of Treatment of Jatropha curcas L. Leachates and Potassium on Growth and Phytochemical Constituents of Wheat (Triticum aestivum L.)

Tomar¹,

Agarwal²

2013

AJPS

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Jatropha curcas L. commonly known as "physic nut" is an exotic species that shows invasive characters. Plantation of Jatropha species is being undertaken at a large scale for biodiesel production. The plants defoliate their leaves twice a year and have been shown to possess certain antinutritional factors. Seeds of Jatropha are removed from mature dried fruits for oil extraction and ovary walls are discarded. For the present experiments leachates from sun dried leaves and ovary walls of Jatropha curcas L. were used for analyzing their possible allelopathic effects on the growth of wheat (Triticum aestivum L. cultivar, MP-4010). Leachate treatments result in slight decrease in growth in terms of plant height, leaf area, biomass, spike length and 100 seed weight of wheat and increase in total phenols, tannins, phytic acid and free amino acids. Results suggest that the leachates of leaf and ovary wall of Jatropha curcas are slightly inhibitory, however the impact does not last long. Nevertheless, continuous plantation may lead to accumulation of damaging constituents which reflects the need for further analysis and experimentation.

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Section: Resultsmentioning

confidence: 99%

Influence of Treatment of Jatropha curcas L. Leachates and Potassium on Growth and Phytochemical Constituents of Wheat (Triticum aestivum L.)

Tomar¹,

Agarwal²

2013

AJPS

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“…Uric acid reaction with micelle-incorporated DPPH was explained by Abuin et al (2002), while Bandoniene et al (2002) detected the activity of radical scavenging compounds in mixed methanol-water solution using DPPH and HPLC-DPPH method. Ionita et al (2000) used water-soluble derivate of DPPH for the study of oxidation kinetics of amino acid.…”

Section: Developments In Dpph Methodsmentioning

confidence: 99%

Genesis and development of DPPH method of antioxidant assay

2011

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α, α-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging method offers the first approach for evaluating the antioxidant potential of a compound, an extract or other biological sources. This is the simplest method, wherein the prospective compound or extract is mixed with DPPH solution and absorbance is recorded after a defined period. However, with the advancement and sophistication in instrumental techniques, the method has undergone various modifications to suit the requirements, even though the basic approach remains same in all of them. This article presents a critical review on various developments to the DPPH method.

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“…DPPH radical scavenging assay was performed according to Bandoniené (2002). Oregano methanol/water extract (0.65 ml) was placed into 25 ml of DPPH methanolic solution c 0 (DPPH) = 6 × 10 -5 mol/dm 3 , and the absorbance at 515 nm was measured after 15 min.…”

Section: Methodsmentioning

confidence: 99%

The influence of gamma-irradiation on the formation of free radicals and antioxidant status of oregano (Origanum vulgare L.)

Horváthová¹,

Suhaj²,

Polovka³

et al. 2007

Czech J. Food Sci.

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The influence of various gamma-radiation dose absorptions on oregano (Origanum vulgare L.) solid samples was monitored by means of electron paramagnetic resonance (EPR) spectroscopy. Further, the antioxidant activity of oregano methanol/water extracts was characterised using DPPH (1,1-diphenyl-2-picrylhydrazyl free radical); thiobarbituric acid reactive substances (TBARS); ferric reducing power (FRP); and total content of phenolic compounds (TPC) assays. EPR spectroscopy was used for the investigation of the influence of the absorbed dose on the character of the paramagnetic structures formed, as well as for their thermal stability and life-time characterisation. EPR spectrum of the reference (non-irradiated) sample represents a broad singlet line with unresolved hyperfine splitting, attributable to Mn(II) ions, upon which the additional sharp EPR signal (g = 2.0022, ΔBpp ≈ 1 mT) is superimposed, assigned to stable semiquinone radicals produced by the oxidation of polyphenolic compounds present in plants. The additional paramagnetic structures of different origin (mostly of cellulose and carbohydrate), possessing diverse thermal stability and life-time, were identified in the gamma-irradiated samples. Immediately after irradiation, a statistically significant increase was observed of the TBARS values and the total content of phenolic compounds in methanol/water oregano extract. The alterations of the antioxidant properties of oregano extracts with the time after the radiation treatment were also monitored. A substantial time-dependent decrease of antioxidant activity was observed, probably as a result of storage, with both irradiated and non-irradiated oregano samples, as obvious from the ferric reducing power test and the content of total phenolic substances. The influence of irradiation and subsequent storage of oregano samples on the DPPH radical-scavenging ability was only negligible.

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Detection and activity evaluation of radical scavenging compounds by using DPPH free radical and on-line HPLC-DPPH methods

Cited by 119 publications

References 0 publications

Influence of Treatment of <i>Jatropha curcas</i> L. Leachates and Potassium on Growth and Phytochemical Constituents of Wheat (<i>Triticum aestivum</i> L.)

Influence of Treatment of <i>Jatropha curcas</i> L. Leachates and Potassium on Growth and Phytochemical Constituents of Wheat (<i>Triticum aestivum</i> L.)

Genesis and development of DPPH method of antioxidant assay

The influence of gamma-irradiation on the formation of free radicals and antioxidant status of oregano (Origanum vulgare L.)

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Detection and activity evaluation of radical scavenging compounds by using DPPH free radical and on-line HPLC-DPPH methods

Cited by 119 publications

References 0 publications

Influence of Treatment of &lt;i&gt;Jatropha curcas&lt;/i&gt; L. Leachates and Potassium on Growth and Phytochemical Constituents of Wheat (&lt;i&gt;Triticum aestivum&lt;/i&gt; L.)

Influence of Treatment of &lt;i&gt;Jatropha curcas&lt;/i&gt; L. Leachates and Potassium on Growth and Phytochemical Constituents of Wheat (&lt;i&gt;Triticum aestivum&lt;/i&gt; L.)

Genesis and development of DPPH method of antioxidant assay

The influence of gamma-irradiation on the formation of free radicals and antioxidant status of oregano (Origanum vulgare L.)

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Product

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Influence of Treatment of <i>Jatropha curcas</i> L. Leachates and Potassium on Growth and Phytochemical Constituents of Wheat (<i>Triticum aestivum</i> L.)

Influence of Treatment of <i>Jatropha curcas</i> L. Leachates and Potassium on Growth and Phytochemical Constituents of Wheat (<i>Triticum aestivum</i> L.)