Detecting Validated Intracellular ROS Generation with 18F-dihydroethidine-Based PET

Waters, Edward C. T.; Baark, Friedrich; Yu, Zilin; Mota, Fernando; Eykyn, Thomas R.; Yan, Ran; Southworth, Richard

doi:10.1007/s11307-021-01683-0

Cited by 4 publications

(3 citation statements)

References 21 publications

(30 reference statements)

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“…DHE is one of the most commonly used superoxide anion fluorescent probes, which can effectively detect ROS levels [56]. After staining with DHE, the mycelia of the ∆Aoprdx2 mutant accumulated more ROS compared with the WT strain (Figure 5A).…”

Section: Aoprdx2 Regulates Ros Accumulation and Endocytosismentioning

confidence: 99%

AoPrdx2 Regulates Oxidative Stress, Reactive Oxygen Species, Trap Formation, and Secondary Metabolism in Arthrobotrys oligospora

Zhao,

Zhu,

Liu

et al. 2024

JoF

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Prdx2 is a peroxiredoxin (Prx) family protein that protects cells from attack via reactive oxygen species (ROS), and it has an important role in improving the resistance and scavenging capacity of ROS in fungi. Arthrobotrys oligospora is a widespread nematode-trapping fungus that can produce three-dimensional nets to capture and kill nematodes. In this study, AoPrdx2, a homologous protein of Prx5, was investigated in A. oligospora via gene disruption, phenotypic analysis, and metabolomics. The deletion of Aoprdx2 resulted in an increase in the number of mycelial septa and a reduction in the number of nuclei and spore yield. Meanwhile, the absence of Aoprdx2 increased sensitivity to oxidative stresses, whereas the ∆Aoprdx2 mutant strain resulted in higher ROS levels than that of the wild-type (WT) strain. In particular, the inactivation of Aoprdx2 severely influenced trap formation and pathogenicity; the number of traps produced by the ∆Aoprdx2 mutant strain was remarkably reduced and the number of mycelial rings of traps in the ∆Aoprdx2 mutant strain was less than that of the WT strain. In addition, the abundance of metabolites in the ∆Aoprdx2 mutant strain was significantly downregulated compared with the WT strain. These results indicate that AoPrdx2 plays an indispensable role in the scavenging of ROS, trap morphogenesis, and secondary metabolism.

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Section: Aoprdx2 Regulates Ros Accumulation and Endocytosismentioning

confidence: 99%

AoPrdx2 Regulates Oxidative Stress, Reactive Oxygen Species, Trap Formation, and Secondary Metabolism in Arthrobotrys oligospora

Zhao,

Zhu,

Liu

et al. 2024

JoF

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“…Ex vivo studies showed that the uptake of [ 18 F] 6 was significantly greater in menadione-treated hearts than in normal heart. Moreover, the higher uptake of [ 18 F] 6 was also associated with concomitant cardiac contractile dysfunction …”

Section: Radiotracers Based On Fluorescent Moleculesmentioning

confidence: 99%

“…Moreover, the higher uptake of [ 18 F]6 was also associated with concomitant cardiac contractile dysfunction. 29 1.2. Radionuclide Labeled Hydrocyanine Derivatives.…”

Section: ■ Introductionmentioning

confidence: 99%

Radiotracers for Nuclear Imaging of Reactive Oxygen Species: Advances Made So Far

Huang

Zhang

2022

Bioconjugate Chem.

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Reactive oxygen species (ROS) are a cluster of highly reactive and short-lived oxygen-containing molecules that lead to metabolic disorders where production exceeds catabolism in an organism. Many specific radiotracers for positron/single-photon emission tomography have been developed to reveal the discrepancy of ROS levels in normal and damaged tissues and further clarify the relationship between ROS and diseases. This review summarizes the advances achieved for the development of ROS radiotracers to date. The structure design, radiosynthesis, and imaging performance of existing radiotracers are discussed with the individual ROS-response mechanisms highlighted.

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Characterization and Validation of Radiotracer Kinetics Using the Langendorff Isolated Perfused Heart

Baark,

Waters,

Eykyn

et al. 2023

Methods in Molecular Biology

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Detecting Validated Intracellular ROS Generation with 18F-dihydroethidine-Based PET

Cited by 4 publications

References 21 publications

AoPrdx2 Regulates Oxidative Stress, Reactive Oxygen Species, Trap Formation, and Secondary Metabolism in Arthrobotrys oligospora

AoPrdx2 Regulates Oxidative Stress, Reactive Oxygen Species, Trap Formation, and Secondary Metabolism in Arthrobotrys oligospora

Radiotracers for Nuclear Imaging of Reactive Oxygen Species: Advances Made So Far

Characterization and Validation of Radiotracer Kinetics Using the Langendorff Isolated Perfused Heart

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