Detecting Single Porphyrin Molecules in a Conically Shaped Synthetic Nanopore

Heins, Elizabeth A; Siwy, Zuzanna S.; Baker, Lane A.; Martin, Charles R.

doi:10.1021/nl050925i

Cited by 257 publications

(292 citation statements)

References 37 publications

Supporting

Mentioning

284

Contrasting

Unclassified

Order By: Relevance

“…The extensive literature on mathematical modelling and numerical simulations of particle translocation through conical and cylindrical nanopores has recently been reviewed [54]. An approximate mathematic model developed for conical pores [55,56] was later adapted for a spherical particle translocating through a nanopipette [31] to calculate the shape and amplitude of the current pulse (figure 4). Similar to conical pores, the pulses are expected to be asymmetrical with the fast initial decrease in current followed by a slow relaxation (tail).…”

Section: (B) Theorymentioning

confidence: 99%

Resistive-pulse and rectification sensing with glass and carbon nanopipettes

Wang

Mirkin

2017

Proc. R. Soc. A.

View full text Add to dashboard Cite

Along with more prevalent solid-state nanopores, glass or quartz nanopipettes have found applications in resistive-pulse and rectification sensing. Their advantages include the ease of fabrication, small physical size and needle-like geometry, rendering them useful for local measurements in small spaces and delivery of nanoparticles/biomolecules. Carbon nanopipettes fabricated by depositing a thin carbon layer on the inner wall of a quartz pipette provide additional means for detecting electroactive species and fine-tuning the current rectification properties. In this paper, we discuss the fundamentals of resistivepulse sensing with nanopipettes and our recent studies of current rectification in carbon pipettes.

show abstract

Section: (B) Theorymentioning

confidence: 99%

Resistive-pulse and rectification sensing with glass and carbon nanopipettes

Wang

Mirkin

2017

Proc. R. Soc. A.

View full text Add to dashboard Cite

show abstract

“…For a conical pore, the change in resistance, ∆R, across the length of the pore, L, is given by equation 1 45 ,…”

Section: Introduction -mentioning

confidence: 99%

“…The size of any biological or inorganic particle that traverses the pore, can be determined by measuring the change in resistance. Heins et al's work used this relationship to monitor and model the translocation events of small molecules 45 . Kozal et al recently derived a similar relationship between the change in resistance within a conical pore using an elastic pore sensor, which closely predicts the ∆R with nanoparticles traversing the pore 46 .…”

Section: Introduction -mentioning

confidence: 99%

Monitoring Aptamer–Protein Interactions Using Tunable Resistive Pulse Sensing

Billinge

Broom²,

Platt

2013

Anal. Chem.

View full text Add to dashboard Cite

-Aptamers are short single-stranded pieces of DNA or RNA capable of binding to analytes with specificity and high affinity. Due to their comparable selectivity, stability and cost, over the last two decades aptamers have started to challenge antibodies in their use on many technology platforms. The binding event often leads to changes in the aptamer's secondary and tertiary structure; monitoring such changes has led to the creation of many new analytical sensors. Here we demonstrate the use of a tunable resistive pulse sensing (TRPS) technology to monitor the interaction between several DNA aptamers and their target -thrombin. We immobilised the aptamers onto the surface of superparamagnetic beads, prior to their incubation with the thrombin protein. The protein binding to the aptamer caused a conformational change resulting in the shielding of the polyanion backbone; this was monitored by a change in the translocation time and pulse frequency of the particles traversing the pore. This signal was sensitive enough to allow the tagless detection of thrombin down to nanomolar levels. We further demonstrate the power of TRPS by performing real time detection and characterisation of the aptamer-target interaction and measuring the association rates of the thrombin protein to the aptamer sequences.

show abstract

“…Track-etched nanopores are fabricated by etching the ion tracks inside organic foils, 1 and they gain more and more attention due to their applications in biosensor, 2,3 DNA sequencing, 4,5 mimicking the ion channel, 6,7 and energy conversion. 8 In all these applications, surface charge is crucially important, since it governs ion transport through the pores, especially when the salt concentration is low.…”

Section: Introductionmentioning

confidence: 99%

Surface charge density of the track-etched nanopores in polyethylene terephthalate foils

Xue

Xie

et al. 2009

Biomicrofluidics

View full text Add to dashboard Cite

Surface charge is one of the most important properties of nanopores, which determines the nanopore performance in many practical applications. We report the surface charge densities of track-etched nanopores, which were obtained by measuring the streaming current and pore conductance, respectively. Experimental results reveal that surface charge densities depend significantly on the salt concentrations. In addition the values obtained with the pore conductance were always several times higher than those calculated with the streaming current, and the gel-like surface layer on the nanopore was considered to be responsible for this discrepancy.

show abstract

Detecting Single Porphyrin Molecules in a Conically Shaped Synthetic Nanopore

Cited by 257 publications

References 37 publications

Resistive-pulse and rectification sensing with glass and carbon nanopipettes

Resistive-pulse and rectification sensing with glass and carbon nanopipettes

Monitoring Aptamer–Protein Interactions Using Tunable Resistive Pulse Sensing

Surface charge density of the track-etched nanopores in polyethylene terephthalate foils

Contact Info

Product

Resources

About