Detecting mislabelling in meat products using PCR–FINS

Abstract

Economically motivated adulteration (EMA) or misrepresentation of meat products is of concern, especially in developing countries, due to obvious health hazards and religious sensitivities. As Indian cooking involves prolonged heat treatments and addition of spices and condiments, species authentication of food, especially meat products, may be challenging. This study evaluated the efficacy of Polymerase Chain Reaction-Forensically Informative Sequencing (PCR-FINS) in meat speciation of highly processed meat. Further the prevalence of mislabelling in processed and deeply cooked meat products being sold in supermarkets and restaurants in a south Indian city was investigated. FINS targeting the mitochondrial cytochrome b gene and the ATP synthase gene was applied to identify meat species of 106 meat products labelled as chicken, beef, carabeef, mutton and pork. Mislabelling was detected in more than half of mutton (52.3%) and carabeef (55.5%), and in under a third (27.2%) of beef products. PCR-FINS is a reliable method for meat species identification even in highly processed food but there is a need for appropriate universal primers which can target all common species used in meat products. This study is the first of its kind from the South Indian state of Kerala.

Canning Processes Reduce the DNA-Based Traceability of Commercial Tropical Tunas

“…The use of DNA barcoding may be limited in highly processed tuna products where DNA could be degraded into smaller fragments that are difficult to amplify [ 39 ]. However, Polymerase Chain Reaction-Forensically Informative Sequencing (PCR-FINS) and mini-DNA barcoding methods were successfully applied for species identification in highly processed meat foods [ 40 ] and in museum specimens [ 41 , 42 ].…”

A Bayesian Approach to Predict Food Fraud Type and Point of Adulteration

“…This study corroborates with Li et al, 2020 [26], and Zhang and Xue, 2016 [27], who identified artificial enhancement as the major food fraud type and was due to usage of unapproved veterinary and human drug residues, or chemicals such as pesticides, food additives, and veterinary that were beyond the approved limits to enhance or prolonged the shelf-life of the food and drink products. Species replacement has been identified as a major problem in meat and poultry products [36,37], seafood [38,39], tubers [40], with 20% to more than 70% of the sampled products in the reported studies were identified as different species compared to the commercial name indicated on the label.…”

Biosensor DNA Elektrokimia untuk Deteksi Makanan Mengandung Babi (Sus scrofa) Menggunakan Screen Printed Carbon Electrode Termodifikasi Emas

“…Pengembangan metode yang lebih cepat dan akurat untuk mengidentifikasi spesies dalam komoditas pangan, seperti mendeteksi daging babi dalam makanan masih terus dilakukan. Berbagai metode analitik yang telah dikembangkan untuk identifikasi babi, diantaranya adalah metode berbasis lipid (Rohman et al, 2011;Stachniuk et al, 2021), metode berbasis protein/Enzyme-Linked Immunosorbent Assay (ELISA) (Adam et al, 2017;Mandli et al, 2018), dan metode berbasis DNA (Ali et al, 2012;Ha et al, 2017;Kim et al, 2016;Soman et al, 2020).…”

“…Sejumlah pengembangan metode Polymerase Chain Reaction (PCR) yang akhir-akhir ini digunakan untuk identifikasi DNA babi, diantaranya adalah PCR-RFLP (Restriction Fragment Length Pholymorphism) (Ali et al, 2011a), spesies spesifik PCR (Hafidz et al, 2020); realtime PCR menggunakan probe spesifik dengan target gen Cyt B (Ali et al, 2012), target D-loop (Kim et al, 2016); PCR -QIAxcel capillary electrophoresis system dengan target Cyt B dan D-loop (Barakat et al, 2014); serta PCR -Forensically Informative Sequencing (PCR-FINS) dengan target gen Cyt B dan ATP synthase (Soman et al, 2020).…”