Detecting Human Papillomavirus in Ocular Surface Diseases

Woods, Mark; Chow, S. P.; Heng, Benjamin; Glenn, Wendy K.; Whitaker, Noel J.; Waring, Dale; Iwasenko, Jenna M.; Rawlinson, William D.; Coroneo, Minas T.; Wakefield, Denis; Girolamo, Nick Di

doi:10.1167/iovs.13-13140

Cited by 43 publications

(32 citation statements)

References 64 publications

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“…The vast majority of HPV types cause benign warts of the skin or genital region (Table 1) (8)(9)(10)(11)(12)(13). Some HPV types have the potential to cause lesions that progress to cancer.…”

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confidence: 99%

Human Papillomavirus Laboratory Testing: the Changing Paradigm

2016

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SUMMARYHigh-risk human papillomaviruses (HPVs) cause essentially all cervical cancers, most anal and oropharyngeal cancers, and some vaginal, vulvar, and penile cancers. Improved understanding of the pathogenesis of infection and the availability of newer tests are changing the approach to screening and diagnosis. Molecular tests to detect DNA from the most common high-risk HPVs are FDA approved for use in conjunction with cytology in cervical cancer screening programs. More-specific tests that detect RNA from high-risk HPV types are now also available. The use of molecular tests as the primary screening tests is being adopted in some areas. Genotyping to identify HPV16 and -18 has a recommended role in triaging patients for colposcopy who are high-risk HPV positive but have normal cytology. There are currently no recommended screening methods for anal, vulvar, vaginal, penile, or oropharyngeal HPV infections. HPV testing has limited utility in patients at high risk for anal cancer, but p16 immunohistochemistry is recommended to clarify lesions in tissue biopsy specimens that show moderate dysplasia or precancer mimics. HPV testing is recommended for oropharyngeal squamous cell tumors as a prognostic indicator. Ongoing research will help to improve the content of future guidelines for screening and diagnostic testing.

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confidence: 99%

Human Papillomavirus Laboratory Testing: the Changing Paradigm

2016

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“…DNA quality and yield are generally better from fresh specimens than preserved specimens, but the unavailability of fresh specimens may be a common roadblock to these studies, as tissue is typically submitted for histopathological examination (Woods et al, 2013). Therefore, FFPE samples represent an important source for retrospective studies (Newkirk et al 2014); however, the use of an adequate fixation process to conserve tissue morphology may negatively affect nucleic acid preservation and may consequently also affect molecular diagnostic procedures (Barcelos et al, 2008).…”

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“…Therefore, a screening PCR assay using FFPE samples should be performed in parallel with reactions that amplify a housekeeping gene of similar size to ensure the DNA is not compromised and is amenable to PCR (Woods et al, 2013). In this study, a PCR assay for equine β-actin (reference gene) was used to confirm that the DNA purification methods were efficient and that the samples contained sufficient amplifiable DNA.…”

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confidence: 99%

Detection of papillomavirus DNA in formalin-fixed paraffin-embedded equine aural plaque samples

Zakia

Basso

Olivo

et al. 2015

Arq. Bras. Med. Vet. Zootec.

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“…Moreover, no putative pRB binding sites (LXCXE) have been identified in equine papilloma viruss (EcPV-2) and 3 E7 amino acid sequences, so E7 is probably not able to modulate pRB and consequently the p16 protein. In human pathology the prevalence of HPV in ocular surface disease varies dramatically, according to variations in the assays used to detect the virus, as well as geography and genetic susceptibility (Woods et al 2013), and the utility of p16INK4a immunoexpression for predicting HPV in ocular surface squamous neoplasia was evaluated but, unlike cervical cancers, it seems that there is no correlation. In fact, the overexpression of p16INK4a in OSSN was significantly associated with HPV (Chauhan et al 2012), while Auw-Haedrich et al (2008) have reported HPV and p16INK4a positivity in conjunctival intraepithelial neoplasias (CIN) but they did not observe any association between p16INK4a and HPV.…”

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confidence: 99%

Equine ocular squamous cell carcinoma: a case report

Drazek

Lew

et al. 2015

Vet. Med. - Czech

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An eight-year-old gelding, Wielkopolski Horse was presented with a pink tumour, which filled the entire palpebral fissure of the left eye. Ultrasonography revealed it to be well-demarcated from the cornea mass, which covered its entire surface. Due to the extensive size of the lesion and the lack of owner's consent to postoperative treatment, it was decided to perform enucleation. A histopathological examination revealed corneal squamous cell carcinoma (CSCC). The primary cause of the tumour has not been established. Immunohistochemistry was negative for p16 immunoreactivity which is typical for the E7 oncoprotein in PV infection, and is suspected of involvement in the aetiopathogenesis of ocular squamous cell carcinoma (OSCC).

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Detecting Human Papillomavirus in Ocular Surface Diseases

Abstract: The low prevalence of HPV-16 in ocular surface disease suggests infection is not a cause but a cofactor in disease development.

Cited by 43 publications

References 64 publications

Human Papillomavirus Laboratory Testing: the Changing Paradigm

Human Papillomavirus Laboratory Testing: the Changing Paradigm

Detection of papillomavirus DNA in formalin-fixed paraffin-embedded equine aural plaque samples

Equine ocular squamous cell carcinoma: a case report

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