Detecting Growth Hormone Abuse in Athletes

Bidlingmaier, Martin; Manolopoulou, Jenny

doi:10.1016/j.ecl.2009.10.006

Cited by 11 publications

(2 citation statements)

References 42 publications

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“…However, the detection of this molecule in blood is very difficult due to its short half-life (15–20 min) [150, 151]. Two tests are currently used for detection of rhGH misuse and abuse, each with their own limitations [152]. Because of these difficulties, our laboratory sought to utilize proteomic studies to identify serum biomarkers corresponding to rhGH use (Table 5) [153].…”

Section: Main Textmentioning

confidence: 99%

The impact of growth hormone on proteomic profiles: a review of mouse and adult human studies

2017

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Growth hormone (GH) is a protein that is known to stimulate postnatal growth, counter regulate insulin’s action and induce expression of insulin-like growth factor-1. GH exerts anabolic or catabolic effects depending upon on the targeted tissue. For instance, GH increases skeletal muscle and decreases adipose tissue mass. Our laboratory has spent the past two decades studying these effects, including the effects of GH excess and depletion, on the proteome of several mouse and human tissues. This review first discusses proteomic techniques that are commonly used for these types of studies. We then examine the proteomic differences found in mice with excess circulating GH (bGH mice) or mice with disruption of the GH receptor gene (GHR−/−). We also describe the effects of increased and decreased GH action on the proteome of adult patients with either acromegaly, GH deficiency or patients after short-term GH treatment. Finally, we explain how these proteomic studies resulted in the discovery of potential biomarkers for GH action, particularly those related with the effects of GH on aging, glucose metabolism and body composition.Electronic supplementary materialThe online version of this article (doi:10.1186/s12014-017-9160-2) contains supplementary material, which is available to authorized users.

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Section: Main Textmentioning

confidence: 99%

The impact of growth hormone on proteomic profiles: a review of mouse and adult human studies

2017

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“…At the 2012 Olympic Games in London, the marker approach to detect hGH abuse was added to the repertoire of anti-doping tests, which was so far limited to the isoform method. [6] The marker approach is the result of the GH 2000 project, a large multi-centre study which was supported by the IOC, the European Union, and two hGH manufacturers. [7] Testing is based on the concentration of the two markers IGF-1 and P-III-nP, which both increase following hGH administration.…”

Section: Introductionmentioning

confidence: 99%

The effect of a period of intense exercise on the marker approach to detect growth hormone doping in sports

Voss

Robinson

Alsayrafi

et al. 2014

Drug Testing and Analysis

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The major objective of this study was to investigate the effects of several days of intense exercise on the growth hormone marker approach to detect doping with human growth hormone (hGH). In addition we investigated the effect of changes in plasma volume on the test. Fifteen male athletes performed a simulated nine-day cycling stage race. Blood samples were collected twice daily over a period of 15 days (stage race + three days before and after). Plasma volumes were estimated by the optimized CO Rebreathing method. IGF-1 and P-III-NP were analyzed by Siemens Immulite and Cisbio Assays, respectively. All measured GH 2000 scores were far below the published decision limits for an adverse analytical finding. The period of exercise did not increase the GH-scores; however the accompanying effect of the increase in Plasma Volume yielded in essentially lower GH-scores. We could demonstrate that a period of heavy, long-term exercise with changes in plasma volume does not interfere with the decision limits for an adverse analytical finding.

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Annual banned‐substance review: analytical approaches in human sports drug testing

Thevis

Kuuranne

Geyer

et al. 2011

Drug Testing and Analysis

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The timely update of the list of prohibited substances and methods of doping (as issued by the World Anti-Doping Agency) is an essential aspect of international anti-doping efforts and represents consensual agreement by expert panels regarding substances and the methods of performance manipulation in sports. The annual banned-substance review for human doping controls critically summarizes recent innovations in analytical approaches; its purpose is to improve the quality of doping controls by reporting emerging and advancing methods that focus on detecting known and recently outlawed substances. This review surveys new and/or enhanced procedures and techniques of doping analysis together with information relevant to doping control that has been published in the literature between October 2009 and September 2010.

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Detecting Growth Hormone Abuse in Athletes

Cited by 11 publications

References 42 publications

The impact of growth hormone on proteomic profiles: a review of mouse and adult human studies

The impact of growth hormone on proteomic profiles: a review of mouse and adult human studies

The effect of a period of intense exercise on the marker approach to detect growth hormone doping in sports

Annual banned‐substance review: analytical approaches in human sports drug testing

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