Detecting genotoxic activity in industrial effluents using the SOS Chromotest microplate assay

Legault, R.; Blaise, C.; Trottier, S.; White, Paul A.

doi:10.1002/(sici)1098-2256(1996)11:2<151::aid-tox11>3.0.co;2-g

Cited by 27 publications

(24 citation statements)

References 35 publications

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“…Consequently, the wastewaters could not be considered, with one exception (Municipal Wastewater 1) the likely source of the mutagenic activity. This lack of a causal relationship contrasts the results reported for other industrial wastewaters and genotoxic assays (Rannug, 1980;de Raat et al, 1985;Møller et al, 1986;Bilawchuck et al, 1989;Houk, 1992;Wong et al, 1994;Rao et al, 1995;Legault et al, 1996).…”

Section: Discussioncontrasting

confidence: 60%

Potential genotoxicity of wastewater‐contaminated pore waters with comparison to sediment toxicity and macrobenthic community composition*

Lewis

Daniels

Moore

et al. 2002

Environmental Toxicology

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The objectives of this survey were to determine the genotoxic potential of sediment pore waters above and below 10 wastewater outfalls and to compare the results to sediment chemical quality guidelines, acute toxicity, and macrobenthic community composition. The focus of the study was on genotoxicity since its occurrence in environmental media below wastewater discharges in the Gulf of Mexico region has not been reported in the scientific literature. Pore waters from 43 sediment samples were assayed using a microbial mutagenicity assay before and after activation with a rat liver microsome mix (S-9). A combination of either direct or activated responses was observed for 40% of the pore waters. Direct, activated, and both direct and activated responses were observed in 5, 26, and 9% of the total samples, respectively. Mutagenic effects were observed below 7 of the 10 outfall areas and in 4 of 6 control areas associated with 6 outfalls. The lowest pore water concentrations causing an activated mutagenic response were statistically similar above and below the outfalls. Mutagenicity occurred more frequently than acute toxicity to estuarine and freshwater invertebrates and there was no consistent relationship between its occurrence and the exceedance of sediment chemical quality guidelines. In contrast, there was some indication that mutagenic activity paralleled low benthic community diversity.

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Section: Discussioncontrasting

confidence: 60%

Potential genotoxicity of wastewater‐contaminated pore waters with comparison to sediment toxicity and macrobenthic community composition*

Lewis

Daniels

Moore

et al. 2002

Environmental Toxicology

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“…In Germany, for example, the umuC test is currently under discussion as an integral part of routine industrial wastewater surveillance. The umuC test, along with other bacterial short-term genotoxicity assays based on the SOS response mechanism (e.g., SOS chromotest [30]), has frequently been used for the screening of complex environmental samples from different origins [17,[31][32][33][34][35][36]. Substantial evidence exists that these tests are valuable tools for the rapid detection of potentially hazardous genotoxicity in environmental samples.…”

Section: Discussionmentioning

confidence: 99%

IDENTIFICATION OF FLUOROQUINOLONE ANTIBIOTICS AS THE MAIN SOURCE OF umuC GENOTOXICITY IN NATIVE HOSPITAL WASTEWATER

Hartmann¹,

Alder²,

Koller³

et al. 1998

Environ Toxicol Chem

107

104

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Abstract-Previous work revealed genotoxic effects in the wastewater of a large university hospital using a bacterial short-term genotoxicity assay, based on a umuC::lacZ fusion gene (umuC assay). These studies ruled out disinfectants and detergents as main causative agents of the genotoxic effects. This paper focuses on specific hospital-related drugs as the cause. The ratio of theoretical mean wastewater concentrations (derived from consumption data) and lowest observable effect concentrations of selected pharmaceuticals were used to calculate umuC induction probabilities. The fluoroquinolone antibiotics Ciproxin and Noroxin showed the highest induction probabilities and exceeded all other investigated drugs by at least one order of magnitude in significance. Antineoplastic drugs, originally thought to be the main effectors, were found to be of marginal significance using the umuC assay. These findings were further supported by investigation of urine samples of hospital patients with the umuC assay. The determination of ciprofloxacin in native hospital wastewater by reversed-phase high-performance liquid chromatography and fluorescence detection revealed concentrations from 3 to 87 g/L. umuC induction factor and ciprofloxacin concentrations in 16 hospital wastewater samples showed a log-linear correlation (r 2 ϭ 0.84, p Ͻ 0.0001). These results suggest that the previously measured umuC genotoxicity in the wastewater of the hospital under investigation is caused mainly by fluoroquinolone antibiotics, especially by ciprofloxacin. On the basis of these findings, the role of the umuC assay as a screening tool for wastewater genotoxicity assessment is discussed.

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“…In Germany, for example, the umuC test is currently under discussion as an integral part of routine industrial wastewater surveillance. The umuC test, along with other bacterial short‐term genotoxicity assays based on the SOS response mechanism (e.g., SOS chromotest [30]), has frequently been used for the screening of complex environmental samples from different origins [17,31‐36]. Substantial evidence exists that these tests are valuable tools for the rapid detection of potentially hazardous genotoxicity in environmental samples.…”

Section: Discussionmentioning

confidence: 99%

Identification of fluoroquinolone antibiotics as the main source of umuC genotoxicity in native hospital wastewater

Hartmann

Alder

Koller

et al. 1998

Enviro Toxic and Chemistry

257

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Previous work revealed genotoxic effects in the wastewater of a large university hospital using a bacterial short‐term genotoxicity assay, based on a umuC::lacZ fusion gene (umuC assay). These studies ruled out disinfectants and detergents as main causative agents of the genotoxic effects. This paper focuses on specific hospital‐related drugs as the cause. The ratio of theoretical mean wastewater concentrations (derived from consumption data) and lowest observable effect concentrations of selected pharmaceuticals were used to calculate umuC induction probabilities. The fluoroquinolone antibiotics Ciproxin® and Noroxin® showed the highest induction probabilities and exceeded all other investigated drugs by at least one order of magnitude in significance. Antineoplastic drugs, originally thought to be the main effectors, were found to be of marginal significance using the umuC assay. These findings were further supported by investigation of urine samples of hospital patients with the umuC assay. The determination of ciprofloxacin in native hospital wastewater by reversed‐phase high‐performance liquid chromatography and fluorescence detection revealed concentrations from 3 to 87 μg/L. umuC induction factor and ciprofloxacin concentrations in 16 hospital wastewater samples showed a log‐linear correlation (r2 = 0.84, p < 0.0001). These results suggest that the previously measured umuC genotoxicity in the wastewater of the hospital under investigation is caused mainly by fluoroquinolone antibiotics, especially by ciprofloxacin. On the basis of these findings, the role of the umuC assay as a screening tool for wastewater genotoxicity assessment is discussed.

show abstract

Detecting genotoxic activity in industrial effluents using the SOS Chromotest microplate assay

Cited by 27 publications

References 35 publications

Potential genotoxicity of wastewater‐contaminated pore waters with comparison to sediment toxicity and macrobenthic community composition*

Potential genotoxicity of wastewater‐contaminated pore waters with comparison to sediment toxicity and macrobenthic community composition*

IDENTIFICATION OF FLUOROQUINOLONE ANTIBIOTICS AS THE MAIN SOURCE OF umuC GENOTOXICITY IN NATIVE HOSPITAL WASTEWATER

Identification of fluoroquinolone antibiotics as the main source of umuC genotoxicity in native hospital wastewater

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