Detecting Genetic Mobility Using a Transposon-Based Marker System in Gamma-Ray Irradiated Soybean Mutants

Hưng, Nguyễn Ngọc; Kim, Dong‐Gun; Lyu, Jae Il; Park, Kyong‐Cheul; Kim, Jung Min; Kim, Jin-Baek; Ha, Bo‐Keun; Kwon, Soon‐Jae

doi:10.3390/plants10020373

Cited by 8 publications

(6 citation statements)

References 53 publications

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“…The activity of transposable elements could be activated by a variety of stresses, such as gamma irradiation [ 63 , 64 , 65 ]. Hung et al [ 66 ] applied a transposon-based marker system to reveal abundant dynamic and active mobility levels of transposons in gamma-ray irradiated soybean mutant lines. Sen et al [ 67 ] detected retrotransposon insertional variations in drought-tolerant wheat mutants obtained by gamma ray irradiation and found high polymorphisms of retrotransposons microsatellite amplified polymorphism (REMAP) markers and inter-retrotransposon amplified polymorphism (IRAP) markers.…”

Section: Discussionmentioning

confidence: 99%

Disease Resistance and Molecular Variations in Irradiation Induced Mutants of Two Pea Cultivars

Deng

Sun

et al. 2022

IJMS

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Induced mutation is useful for improving the disease resistance of various crops. Fusarium wilt and powdery mildew are two important diseases which severely influence pea production worldwide. In this study, we first evaluated Fusarium wilt and powdery mildew resistance of mutants derived from two elite vegetable pea cultivars, Shijiadacaiwan 1 (SJ1) and Chengwan 8 (CW8), respectively. Nine SJ1 and five CW8 M3 mutants showed resistant variations in Fusarium wilt, and the same five CW8 mutants in powdery mildew. These resistant variations were confirmed in M4 and M5 mutants as well. Then, we investigated the genetic variations and relationships of mutant lines using simple sequence repeat (SSR) markers. Among the nine effective SSR markers, the genetic diversity index and polymorphism information content (PIC) values were averaged at 0.55 and 0.46, which revealed considerable genetic variations in the mutants. The phylogenetic tree and population structure analyses divided the M3 mutants into two major groups at 0.62 genetic similarity (K = 2), which clearly separated the mutants of the two cultivars and indicated that a great genetic difference existed between the two mutant populations. Further, the two genetic groups were divided into five subgroups at 0.86 genetic similarity (K = 5) and each subgroup associated with resistant phenotypes of the mutants. Finally, the homologous PsMLO1 cDNA of five CW8 mutants that gained resistance to powdery mildew was amplified and cloned. A 129 bp fragment deletion was found in the PsMLO1 gene, which was in accord with er1-2. The findings provide important information on disease resistant and molecular variations of pea mutants, which is useful for pea production, new cultivar breeding, and the identification of resistance genes.

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Section: Discussionmentioning

confidence: 99%

Disease Resistance and Molecular Variations in Irradiation Induced Mutants of Two Pea Cultivars

Deng

Sun

et al. 2022

IJMS

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“…TRAP method is useful for germplasm genotyping and producing markers associated with desirable agronomic traits in mutation breeding. Hung et al [50] employed this simple rapid method by using the consensus terminal inverted repeat sequences of PONG, miniature inverted-repeat transposable element (MITE)-Tourist (M-t) and MITE-Stowaway (M-s) as target region amplification polymorphism (TE-TRAP) markers to investigate the mobility of TEs in a gamma-irradiated soybean mutant pool. They concluded that MITEs were significant enough to confirm their practical utility as molecular markers for investigating mutant populations which were induced by random variations caused through physical mutagenesis (X-ray or gamma-ray).…”

Section: Present Applications Of Mutation Breeding In Soybeanmentioning

confidence: 99%

Current Strategies and Future of Mutation Breeding in Soybean Improvement

Ayan¹,

Meriç²,

Gümüş³

et al. 2022

Soybean - Recent Advances in Research and Applications

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Soybean, which has many foods, feed, and industrial raw material products, has relatively limited genetic diversity due to the domestication practices which mainly focused on higher yield for many centuries. Besides, cleistogamy in soybean plant reduces genetic variations even further. Improving genetic variation in soybean is crucial for breeding applications to improve traits such as higher yield, early maturity, herbicide, and pest resistance, lodging and shattering resistance, seed quality and composition, abiotic stress tolerance and more. In the 21st century, there are numerous alternatives from conventional breeding to biotechnological approaches. Among these, mutation breeding is still a major method to produce new alleles and desired traits within the crop genomes. Physical and chemical mutagen protocols are still improving and mutation breeding proves its value to be fast, flexible, and viable in crop sciences. In the verge of revolutionary genome editing era, induced mutagenesis passed important cross-roads successfully with the help of emerging supportive NGS based-methods and non-destructive screening approaches that reduce the time-consuming labor-intensive selection practices of mutation breeding. Induced mutagenesis will retain its place in crop science in the next decades, especially for plants such as soybean for which cross breeding is limited or not applicable.

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“…We previously created a mutant diversity pool (MDP) comprising 208 mutant soybean lines from seven wild-type cultivars that were irradiated with 60 Co gamma rays (250 Gy). We subsequently performed target region amplification polymorphism (TRAP) and transposable element (TE)-TRAP marker analyses to clarify genetic associations as well as gene expression and GWAS analyses of agronomic traits and phytochemical contents using our MDP and selected a soybean mutant with high isoflavone contents (average of 7,351 μg g −1 over a 3-year period) [ 51 – 53 ]. Because there has been relatively little breeding for isoflavone contents, a QTL analysis was conducted using only a few varieties (e.g., Angora, Luheidou2, Wayao, and Zhongdou 27) with isoflavone contents of 2,246–3,791 μg g −1 as the parental plants [ 30 , 37 , 39 , 54 ].…”

Section: Introductionmentioning

confidence: 99%

QTL mapping reveals key factors related to the isoflavone contents and agronomic traits of soybean (Glycine max)

Kim,

Seo,

Lee

et al. 2023

BMC Plant Biol

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Background Soybean is a valuable source of edible protein and oil, as well as secondary metabolites that can be used in food products, cosmetics, and medicines. However, because soybean isoflavone content is a quantitative trait influenced by polygenes and environmental interactions, its genetic basis remains unclear. Results This study was conducted to identify causal quantitative trait loci (QTLs) associated with soybean isoflavone contents. A mutant-based F2 population (190 individuals) was created by crossing the Korean cultivar Hwanggeum with low isoflavone contents (1,558 µg g−1) and the soybean mutant DB-088 with high isoflavone contents (6,393 µg g−1). A linkage map (3,049 cM) with an average chromosome length of 152 cM was constructed using the 180K AXIOM® SoyaSNP array. Thirteen QTLs related to agronomic traits were mapped to chromosomes 2, 3, 11, 13, 19, and 20, whereas 29 QTLs associated with isoflavone contents were mapped to chromosomes 1, 3, 8, 11, 14, 15, and 17. Notably, the qMGLI11, qMGNI11, qADZI11, and qTI11, which located Gm11_9877690 to Gm11_9955924 interval on chromosome 11, contributed to the high isoflavone contents and explained 11.9% to 20.1% of the phenotypic variation. This QTL region included four candidate genes, encoding β-glucosidases 13, 14, 17–1, and 17–2. We observed significant differences in the expression levels of these genes at various seed developmental stages. Candidate genes within the causal QTLs were functionally characterized based on enriched GO terms and KEGG pathways, as well as the results of a co-expression network analysis. A correlation analysis indicated that certain agronomic traits (e.g., days to flowering, days to maturity, and plant height) are positively correlated with isoflavone content. Conclusions Herein, we reported that the major QTL associated with isoflavone contents was located in the interval from Gm11_9877690 to Gm11_9955924 (78 kb) on chromosome 11. Four β-glucosidase genes were identified that may be involved in high isoflavone contents of soybean DB-088. Thus, the mutant alleles from soybean DB-088 may be useful for marker-assisted selection in developing soybean lines with high isoflavone contents and superior agronomic traits.

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Detecting Genetic Mobility Using a Transposon-Based Marker System in Gamma-Ray Irradiated Soybean Mutants

Cited by 8 publications

References 53 publications

Disease Resistance and Molecular Variations in Irradiation Induced Mutants of Two Pea Cultivars

Disease Resistance and Molecular Variations in Irradiation Induced Mutants of Two Pea Cultivars

Current Strategies and Future of Mutation Breeding in Soybean Improvement

QTL mapping reveals key factors related to the isoflavone contents and agronomic traits of soybean (Glycine max)

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