Detecting estrus in synchronized heifers-using tailpaint and an aerosol raddle

Macmillan, K.L.; Taufa, V.K.; Barnes, Debra A.; Day, A.M.; Henry, R.I.

doi:10.1016/0093-691x(88)90285-3

Cited by 110 publications

(45 citation statements)

References 16 publications

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“…To aid with estrus detection, tail heads were painted (Impervo) and chalked (All-weather Paintstick). Cows were observed twice daily for signs of estrus, and paint scores were assigned [20]. The day of standing estrus was designated experimental Day 0.…”

Section: Animals and Treatmentmentioning

confidence: 99%

Persistent Dominant Follicle Alters Pattern of Oviductal Secretory Proteins from Cows at Estrus1

Binelli

Hampton

Buhi

et al. 1999

Biology of Reproduction

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The experimental objective was to compare synthesis of oviductal secretory proteins of dairy cows bearing a persistent dominant follicle (PDF) versus a fresh dominant follicle (FDF) at estrus. On Day 7 after synchronized estrus (Day 0), cows received an intravaginal progesterone device and injection of prostaglandin F2alpha (PGF2alpha). On Day 9, cows received an injection of a GnRH agonist (FDF group; n = 3) or received no injection (PDF group, n = 3). On Day 16, all cows received PGF2alpha, and progesterone devices were removed. At slaughter on Day 18 or Day 19, oviducts ipsilateral and contralateral to the dominant follicle were divided into infundibulum, ampulla, and isthmus regions. Explants from oviductal regions were cultured in minimal essential medium supplemented with [3H]leucine for 24 h. Two-dimensional fluorographs of proteins in conditioned media were analyzed by densitometry. Rate of incorporation of [3H]leucine into macromolecules was greater in the infundibulum, ampulla, and isthmus of FDF cows (p < 0.01). Overall, intensities of radiolabeled secretory protein (P) 2 and P13 were greater for FDF than for PDF. In the ampulla, P14 was more intense for FDF while P7 was more intense for PDF. Abundance of P1 in the isthmus was greater for PDF cows. Across regions, P5, P6, P8, P9, and P11 were more intense for PDF than for FDF in the ipsilateral side. In the contralateral side, P19 was more intense for PDF than for FDF, whereas P6, P8, P9, and P11 were more intense for FDF. Differences in biosynthetic activity and in secreted oviductal proteins from cows bearing a PDF may contribute to the decrease in fertility associated with a PDF.

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Section: Animals and Treatmentmentioning

confidence: 99%

Persistent Dominant Follicle Alters Pattern of Oviductal Secretory Proteins from Cows at Estrus1

Binelli

Hampton

Buhi

et al. 1999

Biology of Reproduction

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“…Cyclic, non-lactating Jersey cows (N = 9) were assigned randomly to BSA (control), bCSP or bTP-1 treatment groups and prepared for surgical placement of uterine catheters as described by Knickerbocker et al (1986b) (Macmillan et al, 1988). Cows were allowed access to dirt lot twice daily (08:00 and 20:00 h) for 30 min from Day 15 until 24 h after the last animal was detected in oestrus for observation of oestrus.…”

Section: Other Proceduresmentioning

confidence: 99%

Intrauterine infusion of highly enriched bovine trophoblast protein-1 complex exerts an antiluteolytic effect to extend corpus luteum lifespan in cyclic cattle

Helmer

Hansen²,

Thatcher³

et al. 1989

Reproduction

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“…(1982). Estrus was monitored twice a day using signs such as standing, mounting, mucus discharge and checking tail‐paint scores (Macmillan et al . 1988).…”

Section: Methodsmentioning

confidence: 99%

“…BCS was determined following the method described by Wildman et al (1982). Estrus was monitored twice a day using signs such as standing, mounting, mucus discharge and checking tail-paint scores (Macmillan et al 1988). Milk progesterone concentration was determined twice a week from 3 to 8 weeks postparturition using a solid phase 96-well microtiter plate enzyme immunoassay kit (Ovucheck, Cambridge Life Science, Cambridge, UK).…”

Section: Methodsmentioning

confidence: 99%

Feed intake, lactation performance, blood metabolites and fertility in early lactation dairy cows grazing a timothy pasture

Ohgi

Hara

Ozeki³

et al. 2006

Animal Science Journal

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The present study was conducted to investigate feed intake, milk yield, milk composition, blood metabolites and fertility in early lactation dairy cows grazing a timothy pasture. Fourteen multiparous Holstein cows that calved between 20 May and 19 July were used over a 3‐year period. The stocking rate was 3.6–4.3 cow/ha. Concentrates were fed separately at 9.5–11.5 kg/day per cow (dry matter basis) from 1 to 13 weeks postparturition. Herbage intake was estimated using chromium oxide as an indigestible marker. The mean contents of crude protein, total digestible nutrients and neutral detergent fiber of pasture during the 3‐year study period were 22.3%, 71.8% and 51.7%, and those of total diet were 18.9%, 77.3% and 40.3%, respectively. The mean herbage dry matter intake was 13.0 kg/day from 2 to 13 weeks postparturition during the study, total dry matter intake was 23.7 kg/day, the total digestible nutrients sufficiency rate was 105%, milk yield was 39.7 kg/day, and milk fat percentage was 3.30%. The decrease in bodyweight postparturition was slight. Urea nitrogen concentrations in serum were below 18.3 mg/dL. The mean days to first estrus and days open were 36 and 104 days, respectively. These results indicate that energy deficiency, decrease in bodyweight and fertility in early lactation barely occur when high producing dairy cows are fed enough grazing grass and suitable concentrates.

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Detecting estrus in synchronized heifers-using tailpaint and an aerosol raddle

Cited by 110 publications

References 16 publications

Persistent Dominant Follicle Alters Pattern of Oviductal Secretory Proteins from Cows at Estrus1

Persistent Dominant Follicle Alters Pattern of Oviductal Secretory Proteins from Cows at Estrus1

Intrauterine infusion of highly enriched bovine trophoblast protein-1 complex exerts an antiluteolytic effect to extend corpus luteum lifespan in cyclic cattle

Feed intake, lactation performance, blood metabolites and fertility in early lactation dairy cows grazing a timothy pasture

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