Detecting emerald ash borers (Agrilus planipennis) using branch traps baited with 3D-printed beetle decoys

Domingue, Michael J.; Pulsifer, Drew P.; Lakhtakia, Akhlesh; Berkebile, Jennifer; Steiner, Kim C.; Lelito, Jonathan P.; Hall, Loyal P.; Baker, Thomas C.

doi:10.1007/s10340-014-0598-y

Cited by 33 publications

(23 citation statements)

References 37 publications

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“…Already, our ability to electrically stun feral males suggests the realistic possibility of reporting these electrical events via wireless communication to personnel stationed at remote locations. Furthermore, the electrical stunning obviates the use of sticky surfaces to ensnare males, a cumbersome technique that previously had always been used with such decoy-baited traps (24,25,28). Sticky surfaces function well for chemical attractants, such as pheromones, that are not usually negatively affected by accumulation of the trapped insects; however, when a visual decoy is used as an attractant, the sticky surface can quickly become filled with background visual "clutter" from scores of both target and nontarget insects.…”

Section: Discussionmentioning

confidence: 99%

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Bioreplicated visual features of nanofabricated buprestid beetle decoys evoke stereotypical male mating flights

Domingue¹,

Lakhtakia²,

Pulsifer³

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Recent advances in nanoscale bioreplication processes present the potential for novel basic and applied research into organismal behavioral processes. Insect behavior potentially could be affected by physical features existing at the nanoscale level. We used nanobioreplicated visual decoys of female emerald ash borer beetles (Agrilus planipennis) to evoke stereotypical mate-finding behavior, whereby males fly to and alight on the decoys as they would on real females. Using an industrially scalable nanomolding process, we replicated and evaluated the importance of two features of the outer cuticular surface of the beetle's wings: structural interference coloration of the elytra by multilayering of the epicuticle and fine-scale surface features consisting of spicules and spines that scatter light into intense strands. Two types of decoys that lacked one or both of these elements were fabricated, one type nano-bioreplicated and the other 3D-printed with no bioreplicated surface nanostructural elements. Both types were colored with green paint. The light-scattering properties of the nano-bioreplicated surfaces were verified by shining a white laser on the decoys in a dark room and projecting the scattering pattern onto a white surface. Regardless of the coloration mechanism, the nano-bioreplicated decoys evoked the complete attraction and landing sequence of Agrilus males. In contrast, males made brief flying approaches toward the decoys without nanostructured features, but diverted away before alighting on them. The nano-bioreplicated decoys were also electroconductive, a feature used on traps such that beetles alighting onto them were stunned, killed, and collected. nanofabrication | structural color | spectral emission | visual response | supercontinuum laser

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Section: Discussionmentioning

confidence: 99%

“…3, was produced by a 3D-printing process performed using a Stratasys Dimension 1200es-SST 3D printer (28). The 3D-printed decoys were made of white acrylonitrile butadiene styrene.…”

Section: Methodsmentioning

confidence: 99%

Bioreplicated visual features of nanofabricated buprestid beetle decoys evoke stereotypical male mating flights

Domingue¹,

Lakhtakia²,

Pulsifer³

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

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“…), in contrast, also responds visually to conspecifi cs as a mate-seeking mechanism, and a combination of visual attractants with volatile chemicals from bark extracts of the host trees has been tested as a detection method. The novel use of 3-D printed plastic beetle decoy models deployed on sticky traps has been explored in Pennsylvania and shows potential as a cheap tool for monitoring the beetle (Domingue et al 2015 ). For the same beetle, monitoring of the contents of predatory wasp ( Cerceris fumipennis , the Smoky-winged beetle bandit, Crabronidae) nests led to its fi rst detection in Connecticut (Rutledge et al 2013 ), but such approaches are too laborious for routine uses, although valuable as wider inventory survey tools.…”

Section: Establishment and Spreadmentioning

confidence: 99%

The Stages of Invasion

New

2016

Alien Species and Insect Conservation

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“…Recent studies have highlighted the benefits of 3D printing in terms of cost and time efficiency [12, 13], yet ecologists wanting to implement 3D printing for the first time must still traverse a steep learning curve. Our goal here is to flatten the curve and provide ecologists with a general but sufficient background in 3D printing technology to know what considerations are important when approaching a 3D printing project.…”

Section: Introductionmentioning

confidence: 99%

“…On the low-complexity end of the spectrum, 3D printing has been used to sample two difficult-to-catch, invasive, tree-boring beetle species that cause significant damage. Three-dimensional printed emergence traps make it possible to effectively trap and census invasive ambrosia beetles ( Euwallacea fornicates ) as they emerge from trees [38], while 3D printed decoys placed on standard beetle traps enhanced capture rates of invasive emerald ash borer beetles ( Agrilus planipennis ) [12]. In a more complex application, whale researchers used 3D printing to build an unmanned surface vehicle named SnotBot which allows scientists to get close enough to whales to collect biological samples (Table 1) [39].…”

Section: Introductionmentioning

confidence: 99%

Benefits and limitations of three-dimensional printing technology for ecological research

et al. 2018

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BackgroundEcological research often involves sampling and manipulating non-model organisms that reside in heterogeneous environments. As such, ecologists often adapt techniques and ideas from industry and other scientific fields to design and build equipment, tools, and experimental contraptions custom-made for the ecological systems under study. Three-dimensional (3D) printing provides a way to rapidly produce identical and novel objects that could be used in ecological studies, yet ecologists have been slow to adopt this new technology. Here, we provide ecologists with an introduction to 3D printing.ResultsFirst, we give an overview of the ecological research areas in which 3D printing is predicted to be the most impactful and review current studies that have already used 3D printed objects. We then outline a methodological workflow for integrating 3D printing into an ecological research program and give a detailed example of a successful implementation of our 3D printing workflow for 3D printed models of the brown anole, Anolis sagrei, for a field predation study. After testing two print media in the field, we show that the models printed from the less expensive and more sustainable material (blend of 70% plastic and 30% recycled wood fiber) were just as durable and had equal predator attack rates as the more expensive material (100% virgin plastic).ConclusionsOverall, 3D printing can provide time and cost savings to ecologists, and with recent advances in less toxic, biodegradable, and recyclable print materials, ecologists can choose to minimize social and environmental impacts associated with 3D printing. The main hurdles for implementing 3D printing—availability of resources like printers, scanners, and software, as well as reaching proficiency in using 3D image software—may be easier to overcome at institutions with digital imaging centers run by knowledgeable staff. As with any new technology, the benefits of 3D printing are specific to a particular project, and ecologists must consider the investments of developing usable 3D materials for research versus other methods of generating those materials.Electronic supplementary materialThe online version of this article (10.1186/s12898-018-0190-z) contains supplementary material, which is available to authorized users.

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Detecting emerald ash borers (Agrilus planipennis) using branch traps baited with 3D-printed beetle decoys

Cited by 33 publications

References 37 publications

Bioreplicated visual features of nanofabricated buprestid beetle decoys evoke stereotypical male mating flights

Bioreplicated visual features of nanofabricated buprestid beetle decoys evoke stereotypical male mating flights

The Stages of Invasion

Benefits and limitations of three-dimensional printing technology for ecological research

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