Detecting and Quantifying Biomarkers of Risk for Colorectal Cancer Using Quantum Dots and Novel Image Analysis Algorithms

Bostick, Roberd M.; Kong, Koon Yin; Ahearn, Thomas U.; Chaudry, Qaiser; Cohen, Vaunita; Wang, May D.

doi:10.1109/iembs.2006.4398156

Cited by 3 publications

(3 citation statements)

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“…QDs-based multiplexed biomarker detection has attracted significant attention as a reliable predictor of disease progression and treatment response (45). Bostick et al (46) created a panel of bioconjugated QDs to simultaneously detect and quantify well-recognized prognosticators for colorectal cancer on the same histological sections. QDs conjugated with multiple biomarkers may also be used for simultaneous prognosticator detection to predict medical outcomes in gastric cancer through studying the major components of the tumor stroma, including tumor infiltrating macrophages, tumor microvascular density and neovessel maturity, type IV collagen and matrix metalloproteinase 9 (47-49).…”

Section: Quantum Dots (Qds)mentioning

confidence: 99%

Advances in the application of nanotechnology in the diagnosis and treatment of gastrointestinal tumors

Sun

Fang

et al. 2014

Molecular and Clinical Oncology

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Abstract. Nanotechnology has broad application prospects in the diagnosis and treatment of cancer. Integrating chemistry, engineering, biology and medicine, nanotechnology is a multidisciplinary research field. Nanoscale imaging technology significantly improves the precision and accuracy of tumor diagnosis. Nanocarriers are able to significantly improve the accuracy of dose and targeted drug delivery and reduce the toxic side effects. This review focuses on the emerging roles of these innovative technologies in gastrointestinal cancer diagnostics and therapeutics. Although several problems and barriers are hampering the development of nanodevices, the potential for nanotechnologies to function as multimodal nanotheranostic agents will likely pave the way for the fight against gastrointestinal cancer.

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Section: Quantum Dots (Qds)mentioning

confidence: 99%

Advances in the application of nanotechnology in the diagnosis and treatment of gastrointestinal tumors

Sun

Fang

et al. 2014

Molecular and Clinical Oncology

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“…The method uses specially coated nanocrystals (around 1-10 nm in diameter), called quantum dots, instead of the chromogen 20,21 . Nanocrystal quantum dots have the property of being excited by any type or wavelength of light to emit light in a very thin fluorescence spectrum ( Figure 2C).…”

Section: Nanocrystal Quantum Dotsmentioning

confidence: 99%

Novel Platforms of Multiplexed Immunofluorescence for Study of Paraffin Tumor Tissues

Parra¹

2017

J Cancer Treat & Diagnosis

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Multiplexed immunofluorescence (IF) methods to detect simultaneously different molecules are revolutionizing immunohistochemistry (IHC) in the last years. These new technologies can be valuable for tumor examination in formalin-fixed paraffin-embedded (FFPE) specimens, and for improved new treatment discoveries and translational cancer studies. The aim of this minireview is to highlight the recent methodologies that using multiplexed IF to study simultaneous proteins identification in FFPE tumor tissues to clinical research and potential translational analysis. Multiplexed IF methods, which permit the identification of up to 4 proteins at the same time, have been increased in the last years the abilities of study cells by cells and their spatial distribution in several tumor tissues. Although, most of the old platforms are not more used after the powerful multiplex IHC methods are continue growing, the basis of these old methodologies have helped to improve the new technologies. Associated with image analysis software's these technologies can be improved to performance high throughput assay to study these specimens. Each multiplexed IF technique, detailed herein, is associated with important advantages in cancer study as well as translational research studies.

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“…Compared with conventional organic dyes and fluorescent proteins, QDs have unique optical properties such as broad absorption spectra, narrow and symmetrical emission spectra, superior signal brightness, and resistance to photobleaching. 19,20 At present, QD-based immunofluorescence imaging has become a trend for biological detection, especially for the detection of multiple biomarkers in cell biology and in vivo imaging. [21][22][23] The aim of the study reported here was to investigate the roles of AR and TLR4 in the development of DN and compare the techniques of quantum dot-based immunohistochemistry (QD-IHC) and conventional IHC for the detection of AR and TLR4 in cells and tissues.…”

Section: Introductionmentioning

confidence: 99%

Dual-color immunofluorescent labeling with quantum dots of the diabetes-associated proteins aldose reductase and Toll-like receptor 4 in the kidneys of diabetic rats

Liu

Lian³

et al. 2015

IJN

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Diabetes is one of the major chronic diseases diagnosed worldwide with a common complication of diabetic nephropathy (DN). There are multiple possible mechanisms associated with DN. Aldose reductase (AR) and Toll-like receptor 4 (TLR4) may be involved in the occurrence and development of DN. Here, we describe the distribution of AR and TLR4 in cells and renal tissues of diabetic rats through a quantum dot (QD)-based immunofluorescence technique and conventional immunohistochemistry. As a new type of nanosized fluorophore, QDs have been recognized in imaging applications and have broad prospects in biomedical research. The results of the reported study demonstrate that both the AR and the TLR4 proteins were upregulated in the renal tissues of diabetic rats. Further, to explore the relationship between AR and TLR4 in the pathogenesis of DN, a dual-color immunofluorescent labeling technique based on QDs was applied, where the expressions of AR and TLR4 in the renal tissues of diabetic rats were simultaneously observed – for the first time, as far as we are aware. The optimized QD-based immunofluorescence technique has not only shown a satisfying sensitivity and specificity for the detection of biomarkers in cells and tissues, but also is a valuable supplement of immu-nohistochemistry. The QD-based multiplexed imaging technology provides a new insight into the mechanistic study of the correlation among biological factors as well as having potential applications in the diagnosis and treatment of diseases.

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Detecting and Quantifying Biomarkers of Risk for Colorectal Cancer Using Quantum Dots and Novel Image Analysis Algorithms

Cited by 3 publications

References 0 publications

Advances in the application of nanotechnology in the diagnosis and treatment of gastrointestinal tumors

Advances in the application of nanotechnology in the diagnosis and treatment of gastrointestinal tumors

Novel Platforms of Multiplexed Immunofluorescence for Study of Paraffin Tumor Tissues

Dual-color immunofluorescent labeling with quantum dots of the diabetes-associated proteins aldose reductase and Toll-like receptor 4 in the kidneys of diabetic rats

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Detecting and Quantifying Biomarkers of Risk for Colorectal Cancer Using Quantum Dots and Novel Image Analysis Algorithms

Cited by 3 publications

References 0 publications

Advances in the application of nanotechnology in the diagnosis and treatment of gastrointestinal tumors

Advances in the application of nanotechnology in the diagnosis and treatment of gastrointestinal tumors

Novel Platforms of Multiplexed Immunofluorescence for Study of Paraffin Tumor Tissues

Dual-color immunofluorescent labeling with quantum dots of the diabetes-associated proteins aldose reductase and Toll-like receptor 4 in&nbsp;the&nbsp;kidneys of diabetic rats

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Dual-color immunofluorescent labeling with quantum dots of the diabetes-associated proteins aldose reductase and Toll-like receptor 4 in the kidneys of diabetic rats