Detectable levels of eHSP72 in plasma are associated with physical activity and antioxidant enzyme activity levels in hypertensive subjects

Abstract

Previous studies reported that extracellular HSP72 (eHSP72) correlates with poor prognosis, markers of vascular dysfunction, and the severity of cardiovascular diseases, associated with a systemic oxidative and inflammatory profile. On the other hand, eHSP72 may represent immune-regulatory signaling that is related to exercise benefits, but the association between physical activity levels and eHSP72 levels is not established. Thus, since regular physical activity may avoid oxidative stress and inflammation, we investigate whether detectable levels of eHSP72 in plasma are associated with physical activity and antioxidant enzyme activity levels in hypertensive subjects. Physical activity levels of hypertensive subjects (n = 140) were measured by triaxial movement sensor pedometer for 24 h during 5 consecutive days. One day after, blood was collected into heparinized tubes for oxidative stress analyses (catalase-CAT and superoxide dismutase-SOD activities and malondialdehyde levels) or in disodium EDTA tubes for eHSP72 assays. Thus, hypertensive subjects were classified as physically inactive (< 10,000 footsteps/day) or active (> than 10,000 footsteps/day) and according detectable or not detectable eHSP72 levels in plasma, performing the inactive/eHSP72 − , active/eHSP72 − , inactive/eHSP72 + , and active/eHSP72 + groups. We found that detectable levels of eHSP72 in plasma were associated with physical activity levels and low oxidative stress profile (Higher CAT and SOD activities and low malondialdehyde levels). eHSP72 levels can be used as a biomarker of the amount of physical activity necessary to improve antioxidant defense and thus cardiovascular health in hypertensive subjects.

Effects of High-Fat Diet on eHSP72 and Extra-to-Intracellular HSP70 Levels in Mice Submitted to Exercise under Exposure to Fine Particulate Matter

“…Since HFD mice were not able to swim for 20 minutes, the lower eHSP70 levels in plasma may represent an insufficient accumulation of exercise effort (time × load). These data are in agreement with the hypothesis that a minimum amount of exercise effort is necessary to promote health effects [50]. Thus, the minimum of level physical activity may not be reached due early fatigue and this represents a key limitation in the obese condition.…”

Tracking Biomarker Responses to Exercise in Hypertension

“…They are associated with less lowering of diastolic blood pressure. However, in 140 hypertensive patients assessed with pedometers for 5 consecutive days, those with > 10,000 steps a day had significantly less oxidative stress (higher CAT and SOD levels and low malondialdehyde levels) [ 39 ]. A recent meta-analysis of the efficacy of Tai Chi on HTN was conducted in 9 randomized controlled trials with exercise interventions of 1.5 to 6 months.…”