Detección molecular de Flavivirus en sueros sanguíneos de roedores capturados en Yucatán, México

Torres–Castro, Marco; Poot-Pérez, Martha; Moguel-Lehmer, Carlos; Reyes-Hernández, Bibiana; Panti–May, Jesús Alonso; Noh-Pech, Henry; Hernández-Betancourt, Silvia F.; Medina-Espinosa, N.; Puerto, Fernando I.

doi:10.15381/rivep.v28i2.12053

Cited by 4 publications

(5 citation statements)

References 5 publications

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“…Our analysis did not consider some other confirmed positive mammals as potential hosts due to the fact that they are poorly represented in the data set, including rodents of the genera Rattus and Mus , in spite of the fact that they are associated with MBFVs in urban and rural sites in Mexico [ 15 , 65 , 69 ]. Similarly, for domestic mammals and other potential non-mammalian warm-blooded hosts [ 65 ].…”

Section: Discussionmentioning

confidence: 99%

Using Data Mining and Network Analysis to Infer Arboviral Dynamics: The Case of Mosquito-Borne Flaviviruses Reported in Mexico

Sotomayor-Bonilla

Callejo-Canal

González‐Salazar

et al. 2021

Insects

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Given the significant impact of mosquito-borne flaviviruses (MBFVs) on both human and animal health, predicting their dynamics and understanding their transmission cycle is of the utmost importance. Usually, predictions about the distribution of priority pathogens, such as Dengue, Yellow fever, West Nile Virus and St. Louis encephalitis, relate abiotic elements to simple biotic components, such as a single causal agent. Furthermore, focusing on single pathogens neglects the possibility of interactions and the existence of common elements in the transmission cycles of multiple pathogens. A necessary, but not sufficient, condition that a mosquito be a vector of a MBFV is that it co-occurs with hosts of the pathogen. We therefore use a recently developed modeling framework, based on co-occurrence data, to infer potential biotic interactions between those mosquito and mammal species which have previously been identified as vectors or confirmed positives of at least one of the considered MBFVs. We thus create models for predicting the relative importance of mosquito species as potential vectors for each pathogen, and also for all pathogens together, using the known vectors to validate the models. We infer that various mosquito species are likely to be significant vectors, even though they have not currently been identified as such, and are likely to harbor multiple pathogens, again validating the predictions with known results. Besides the above “niche-based” viewpoint we also consider an assemblage-based analysis, wherein we use a community-identification algorithm to identify those mosquito and/or mammal species that form assemblages by dint of their significant degree of co-occurrence. The most cohesive assemblage includes important primary vectors, such as A. aegypti, A. albopictus, C. quinquefasciatus, C. pipiens and mammals with abundant populations that are well-adapted to human environments, such as the white-tailed deer (Odocoileus virginianus), peccary (Tayassu pecari), opossum (Didelphis marsupialis) and bats (Artibeus lituratus and Sturnira lilium). Our results suggest that this assemblage has an important role in the transmission dynamics of this viral group viewed as a complex multi-pathogen-vector-host system. By including biotic risk factors our approach also modifies the geographical risk profiles of the spatial distribution of MBFVs in Mexico relative to a consideration of only abiotic niche variables.

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Section: Discussionmentioning

confidence: 99%

Using Data Mining and Network Analysis to Infer Arboviral Dynamics: The Case of Mosquito-Borne Flaviviruses Reported in Mexico

Sotomayor-Bonilla

Callejo-Canal

González‐Salazar

et al. 2021

Insects

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“…There is no molecular evidence of MBFV, or evidence of antibody production against DENV-2, in wild rodents from Mexico, although there are reports of DENV in urban rodents ( Mus musculus and Rattus rattus ) [ 8 , 21 ], demonstrating that environmental heterogeneity may directly affect MBFV circulation in these hosts. We found no molecular evidence of MBFVs in rodent samples or evidence of antibody production against DENV.…”

Section: Discussionmentioning

confidence: 99%

“…Also, in the USA, SLEV and WNV have been reported in cricetid ( Sigmodon hispidus , Oryzomys palustris ) and sciurid rodents ( Tamiasciurus hudsonicus , Tamias striatus ) [ 4 , 22 ]. None of the wild rodent species tested in this study were included in past surveys [ 8 , 21 ].…”

Section: Discussionmentioning

confidence: 99%

Survey of mosquito-borne flaviviruses in the Cuitzmala River Basin, Mexico: do they circulate in rodents and bats?

et al. 2018

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BackgroundRNA viruses commonly infect bats and rodents, including mosquito-borne flaviviruses (MBFV) that affect human and animal health. Serological evidence suggests past interactions between these two mammalian orders with dengue viruses (DENV), West Nile virus (WNV), and yellow fever virus (YFV). Although in Mexico there are reports of these viruses in both host groups, we know little about their endemic cycles or persistence in time and space.MethodsRodents and bats were captured at the Cuitzmala River Basin on the Pacific coast of Jalisco state, Mexico, where MBFV, such as DENV, have been reported in both humans and bats. Samples were taken during January, June, and October 2014, at locations adjacent to the river. Tissue samples were collected from both bats and rodents and serum samples from rodents only. Highly sensitive serological and molecular assays were used to search for current and past evidence of viral circulation.ResultsOne thousand nine hundred forty-eight individuals were captured belonging to 21 bat and 14 rodent species. Seven hundred sixty-nine liver and 764 spleen samples were analysed by means of a specific molecular protocol used to detect flaviviruses. Additionally, 708 serum samples from rodents were examined in order to demonstrate previous exposure to dengue virus serotype 2 (which circulates in the region). There were no positive results with any diagnostic test.DiscussionTo our knowledge, this is the first survey of rodents and only the second survey of bats from the Pacific Coast of Mexico in a search for MBFV. We obtained negative results from all samples. We validated our laboratory tests with negative and positive controls. Our findings are consistent with other empirical and experimental studies in which these mammalian hosts may not replicate mosquito-borne flaviviruses or present low prevalence.ConclusionsTrue-negative results are essential for the construction of distribution models and are necessary to identify potential areas at risk. Negative results should not be interpreted as the local absence of MBFV in the region. On the contrary, we need to establish a long-term surveillance programme to find MBFV presence in the mosquito trophic networks, identifying the potential role of rodents and bats in viral dynamics.

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“…Initially, a semi-nested PCR was performed to detect the Flavivirus genus (Torres-Castro et al, 2017). With the cDNA positive to this first reaction, separate reactions were performed to detect DENV 1-4, ZIKV and WNV infections.…”

mentioning

confidence: 99%

West Nile and Zika viruses in bats from a suburban area of Merida, Yucatan, Mexico

Torres–Castro

Noh-Pech

Hernández-Betancourt

et al. 2021

Zoonoses and Public Health

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With approximately 1,386 species distributed worldwide, bats (order Chiroptera) are the second most numerous and diverse mammalian taxon (Burgin et al., 2018). These animals are fundamental to the study of viruses with relevance to public health; however, their epidemiological role in the transmission cycles is unclear. The Flavivirus genus, which includes dengue (DENV 1-4), Zika (ZIKV) and West Nile (WNV) viruses, is one of the most studied viral genera in bats (Fagre & Kading, 2019).The Yucatan state is an area where some Flavivirus infect

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Detección molecular de Flavivirus en sueros sanguíneos de roedores capturados en Yucatán, México

Cited by 4 publications

References 5 publications

Using Data Mining and Network Analysis to Infer Arboviral Dynamics: The Case of Mosquito-Borne Flaviviruses Reported in Mexico

Using Data Mining and Network Analysis to Infer Arboviral Dynamics: The Case of Mosquito-Borne Flaviviruses Reported in Mexico

Survey of mosquito-borne flaviviruses in the Cuitzmala River Basin, Mexico: do they circulate in rodents and bats?

West Nile and Zika viruses in bats from a suburban area of Merida, Yucatan, Mexico

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