Detección histológica y molecular de Helicobacter pylori y genotipificación con base en los genes de virulencia babA2 e iceA en pacientes con patología gástrica benigna

H, Carlos Alberto Jaramillo; Montealegre, María Camila; G, Giovanna Parra

doi:10.4067/s0716-10182010000200002

Cited by 4 publications

(2 citation statements)

References 36 publications

(41 reference statements)

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“…In Colombia, there have been some studies reporting the frequency of vacA, cagA, and iceA, (Cittelly et al, 2002) babA2 and iceA, (Montealegre et al, 2009) alleles of vacA (López et al, 2009) or babA2, oipA and cagE (Quiroga et al, 2005). Considering that gastric cancer is a leading cause of death, our objective was to determine the association and prevalence of cagA, vacA, iceA and babA2 genes in patients with functional dyspepsia, due to the significance of these virulence factors in H. pylori pathogenicity (Atherton, 1998;Van Doorn et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Prevalence of cagA, vacA, babA2 and iceA Genes in Helicobacter pylori Strains Isolated from Colombian Patients with Functional Dyspepsia

Arévalo-Galvis¹,

Trespalacios-Rangel²,

Otero³

et al. 2012

Pol J Microbiol

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The clinical outcome of Helicobacter pylori infection has been particularly associated with virulence genotypes. These genotypes are useful as molecular markers in the identification of patients that are infected and at high risk for developing more severe gastric pathologies. Our main objective was to determine the prevalence of virulence genotypes cagA, vacA, iceA and babA2 of H. pylori, in patients with functional dyspepsia who are infected with the bacteria. H. pylori genotypes babA2 and cagA as well as vacA and iceA allelic variants were identified by PCR in 122 isolates resulting from 79 patients with functional dyspepsia. A high prevalence of genes cagA+ (71%), vacAs1am1 (34%), babA2 (57%) and iceA1 (87%) was found. The most frequent combined genotype found were cagA+/vacAs1am1/babA2+/iceA1 and cagA-/vacAs1am1/babA2+/iceA1, regardless of any family history of gastric cancer or MALT lymphoma. The very virulent genotype cagA+/vacAs1am1/babA2+/iceA1 prevailed in the studied patients with functional dyspepsia. Our results provide information about the prevalence of four of the more important virulent factors and constitute new evidence on the prevalence of the most virulent H. pylori genotype in patients with functional dyspepsia.

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Section: Introductionmentioning

confidence: 99%

Prevalence of cagA, vacA, babA2 and iceA Genes in Helicobacter pylori Strains Isolated from Colombian Patients with Functional Dyspepsia

Arévalo-Galvis¹,

Trespalacios-Rangel²,

Otero³

et al. 2012

Pol J Microbiol

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“…[47][48][49] In Ibagué, located in the Andean mountain region with high GC mortality rates, 12,13,50,51 only studies with small samples size have been carried out, analyzing cagA and the s and m regions of vacA. [40][41][42]52 In the present study we aimed to do a more comprehensive analysis of genotypes in virulence genes in larger groups of patients.…”

Section: Discussionmentioning

confidence: 99%

Infection with Helicobacter Pylori Presenting the vacA s2m2 haplotype is Strongly Associated with Protection Against Gastric Cancer

Guevara-Tique¹,

Castro-Valencia²,

Olaya³

et al. 2021

Preprint

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Background: Infection with Helicobacter pylori is recognized as the main risk factor for gastric cancer (GC); the clinical outcome of this infection is variable and partially depends on the virulence of the infective strain. This study characterizes H. pylori virulence genes in patients with diverse gastric lesions, from preneoplasia to GC, from a South American region with high GC mortality rates.Methods: We studied the virulence profiles of H. pylori strains to colonize the antrum of 318 patients with non-atrophic gastritis (NAG), 58 patients with preneoplastic lesions (PN), and 90 with GC from Ibagué, Colombia. The presence of 16S rDNA, the cagA and cagE genes, and the vacA s1, s2, m1, and m2 alleles were determined by PCR.Results: H. pylori infection was detected in 44% of all patients, 41.2% in NAG, 43.1% in PN and 54.4% of GC patients (p= 0.0813). cagA and cagE genes were significantly more frequent in and GC than in NAG (p= <.0001). The vacA s1m1 haplotype was significantly more frequent in PN (68%) and GC (65.3%) than in NAG (37.4%). The frequency of vacA s2m2 haplotype decreased significantly from NAG (42.7%) to PN (12%) and this to GC (4.1%). A total of 23 different genotypes were identified, with cagA+/cagE+/vacA s1m1 (84/205) as the more frequent in PN and GC and cagA-/cagE-/vacA s2m2 in NAG (49/205).Conclusions: In the population studied, vacA s2m2 was identified as a significant marker for protection against PN and GC, and genotype cagA+/cagE+/vacA s1m1 as a marker for increased GC risk. We also found that patients with PN and GC had a higher frequency of cagA+/cagE+/vacA s1m1 H. pylori strains known to be aggressive.

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Highly clarithromycin-resistant Helicobacter pylori infection in asymptomatic children from a rural community of Cajamarca-Peru

et al. 2018

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ObjectiveThe objective of this study was to determine the prevalence of clarithromycin-resistant Helicobacter pylori in asymptomatic children in a rural community of Cajamarca (northern Peru).ResultsHelicobacter pylori was detected in 17.2% (49/285) of the samples. Unboiled water consumption the most frequent associated factor in patients with positive PCR for H. pylori infection (93.9%). Clarithromycin resistant mutations were found in 79.6% (39/49) of the positive samples for H. pylori. The most frequent mutation was A2142G (46.9%), followed by the double-mutation A2142G–A2143G (28.6%).

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Detección histológica y molecular de Helicobacter pylori y genotipificación con base en los genes de virulencia babA2 e iceA en pacientes con patología gástrica benigna

Cited by 4 publications

References 36 publications

Prevalence of cagA, vacA, babA2 and iceA Genes in Helicobacter pylori Strains Isolated from Colombian Patients with Functional Dyspepsia

Prevalence of cagA, vacA, babA2 and iceA Genes in Helicobacter pylori Strains Isolated from Colombian Patients with Functional Dyspepsia

Infection with Helicobacter Pylori Presenting the vacA s2m2 haplotype is Strongly Associated with Protection Against Gastric Cancer

Highly clarithromycin-resistant Helicobacter pylori infection in asymptomatic children from a rural community of Cajamarca-Peru

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