Detecção molecular de fungos fitopatogênicos associados às sementes de soja

Abstract

Ao Itamar Zatarin de Araújo pelo companheirismo, apoio, paciência e colaboração. À minha Família pelo amor incondicional, apoio e compreensão. A todos que direta ou indiretamente contribuíram para a realização deste trabalho.

Detection and quantification of <i>Colletotrichum abscissum</i> from leaves of budwood increase block and citrus nursery plants by real time PCR

“…As well, qPCR for citrus DNA detection was carried out in total volume of 12 µL per reaction containing 30 ng µL of DNA sample, 3.6 µM of each primer, 6 µL of Path-ID™ qPCR Master Mix (Thermo Fisher Scientific) and 1.8 µM of TaqMan probe.The thermocycler Applied Biosystems 7500 Fast Real-Time PCR System (Thermo Fisher Scientific) was programmed for one cycle at 95 °C for 10 minutes, followed by 45 cycles at 95 °C for 15 seconds and 60 °C for 60 seconds. This volume of reaction has been effective in detecting pathogenic fungi by qPCR using TaqMan system(PEREIRA, 2013;RAMIRO, 2015).…”