Detecção e identificação de Eimeria sp em galinhas caipiras produzidas no município de Mineiros, Goiás

Noronha, Patrícia Calazans de; Carrijo, Daniel Lima; Santos, Gildomar Alves dos; Cardozo, Stanislau Parreira

doi:10.34117/bjdv6n7-139

Cited by 1 publication

(1 citation statement)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, although E. acervulina, E. brunetti, E. maxima, E. mitis/E. mivati, E. necatrix, E. praecox, and E. tenella have been identified based on morphological and morphometric data (Luchese et al, 2007;Noronha et al, 2020;Silva et al, 2022), no studies so far have focused on the molecular identification of Eimeria spp. in APPS in Brazil.…”

Section: Introductionmentioning

confidence: 99%

Identification of Eimeria spp. in domestic chickens raised in alternative poultry production systems in the State of São Paulo, Brazil

Soares Júnior,

Itoyama,

Beretta

et al. 2023

Rev. Bras. Parasitol. Vet.

View full text Add to dashboard Cite

The objective of this study was to identify Eimeria spp. in alternative poultry production systems (APPS) in the State of São Paulo, Brazil. Fecal samples (168) and DNA extracted from fecal samples obtained in APPS located in different Municipalities in the State of São Paulo (93) were examined by microscopy or genera-specific PCR (ITS-1 locus). Samples positive for Eimeria spp. were examined using Eimeria lata, Eimeria nagambie, and Eimeria zaria species-specific PCR protocols (ITS-2 locus) and another E. lata-specific PCR (candidate IMP1 genomic locus) followed by molecular cloning (E. lata and E. zaria ITS-2 amplicons) and genetic sequencing. All positive DNA samples were also submitted to genera-specific nested PCR (18S rRNA gene) followed by next-generation sequencing to identify Eimeria spp. Eimeria nagambie, E. zaria, and Eimeria sp. were identified by ITS2-targeted species-specific PCRs and genetic sequencing. Next-generation sequencing identified, in order of prevalence: E. nagambie; Eimeria acervulina; Eimeria mivati; Eimeria praecox; Eimeria brunetti; Eimeria mitis; Eimeria sp.; Eimeria maxima; E. zaria, and Eimeria necatrix/tenella. Our results confirmed, for the first time in Brazil, the identification of E. nagambie, E. zaria, and Eimeria spp. ITS-2 and 18S rRNA gene sequences not yet described in Brazil.

show abstract

Section: Introductionmentioning

confidence: 99%

Identification of Eimeria spp. in domestic chickens raised in alternative poultry production systems in the State of São Paulo, Brazil

Soares Júnior,

Itoyama,

Beretta

et al. 2023

Rev. Bras. Parasitol. Vet.

View full text Add to dashboard Cite

show abstract

Detecção e identificação de Eimeria sp em galinhas caipiras produzidas no município de Mineiros, Goiás

Abstract: Detecção e identificação de Eimeria sp em galinhas caipiras produzidas no município de Mineiros, Goiás Detection and identification of Eimeria sp in free-range chickens produced in Mineiros county, Goiás

Cited by 1 publication

References 4 publications

Identification of Eimeria spp. in domestic chickens raised in alternative poultry production systems in the State of São Paulo, Brazil

Identification of Eimeria spp. in domestic chickens raised in alternative poultry production systems in the State of São Paulo, Brazil

Contact Info

Product

Resources

About