Detecção do genótipo 4 do vírus da hepatite C em Salvador, BA

Zarife, Maria Alice Sant'Anna; Oliveira, Eline Carvalho Pimentel de; Romeu, Jane Maria Santos Leal; Reis, Mitermayer Galvão dos

doi:10.1590/s0037-86822006000600012

Cited by 8 publications

(7 citation statements)

References 9 publications

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“…When comparing different regions of Brazil, genotype 1 is more prevalent in the North, genotype 2 is more prevalent in the Central-West, especially in Mato Grosso State, and genotype 3 is more prevalent in the South region. Conversely, genotypes 4 and 5 are rarely found, with cases described mainly in São Paulo and only one case reported in Bahia 8,9 .…”

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confidence: 99%

Prevalence of hepatitis C virus genotypes in the State of Pará, Brazil

Guimarães

Melo

Ferreira³

et al. 2018

Rev. Soc. Bras. Med. Trop.

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confidence: 99%

Prevalence of hepatitis C virus genotypes in the State of Pará, Brazil

Guimarães

Melo

Ferreira³

et al. 2018

Rev. Soc. Bras. Med. Trop.

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“…However, genotype 4 of the virus was recently described in Salvador, Bahia, Northeastern area of Brazil (19). …”

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confidence: 99%

Distribution of Hepatitis C virus (HCV) genotypes in seropositive patients in the state of Alagoas, Brazil

Gonzaga¹,

Rodart²,

Reis³

et al. 2008

Braz. J. Microbiol.

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We determined the frequency of hepatitis C virus (HCV) genotypes in anti-HCV seropositive patients in the state of Alagoas, Brazil, by means of nested-reverse transcription-polymerase chain reaction (RT-nested-PCR) followed by restriction fragment length polymorphism (RFLP) of amplified fragments of the 5´NCR. The nested-PCR with genotype-specific primers from the core region was carried out when detection was not possible by the first approach. Detectable HCV-RNA was present in 115 (74.7%) of 154 serum samples. Genotype 1 was the most frequent (77.4%), against 20.9% of genotype 3 and 0.8% of genotype 2. Subtype 1b was predominant (65.2%), followed by subtypes 1a (8.7%), and 3a (6.1%). Coinfection (1a/3a) was detected in 0.8% of the samples. Indeed, there was no significant differences in the prevalence of genotype 1 compared to what has been obtained from anti-HCV seropositive patients from other locations in Brazil. Here we report for the first time the genotype 2 in the state of Alagoas.

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“…Previous data about HCV genotypes in Brazil found types 1, 2, 3, 4 and 5. In all regions genotype 1 was the most frequent, whereas type 4 and 5 are rare ( [7] , [19] , [20] , [21] ). The similarities between the pattern of HCV genotypes distribution in Brazil and that found in many European countries have been pointed out.…”

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confidence: 92%

One-step real-time PCR assay for detection and quantification of RNA HCV to monitor patients under treatment in Brazil

Andrade

Rocha

Fontana-Maurell

et al. 2018

The Brazilian Journal of Infectious Diseases

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The Brazilian Public Health Service provides pegylated alfa-interferon free-of-charge to treat patients infected with HCV. The primary goal of HCV therapy is the long-term elimination of HCV from the blood to reduce the risk of HCV associated complications and death. Patient viremia affects treatment duration and response, thus influencing clinical decisions. We developed a high-throughput method to perform the quantification of RNA hepatitis C virus virus load in plasma samples to monitor patients under treatment. The method is based on a duplex detection, in a one-step real-time RT-PCR assay which has been validated according to the rules established by the official Brazilian regulatory agency (ANVISA). This new method was compared to a commercial kit (Cobas/Taqman HCV Test v2.0 - Roche), showing virus load results with significant correlation between them (p=0.012) using commercial and clinical panels. In addition, 611 samples from patients treated with αPEG-IFN from different regions of Brazil were analyzed. Our one-step real-time RT-PCR assay demonstrated good performance in viral load measurement and in treatment course monitoring, with acceptable sensitivity and specificity values.

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Detecção do genótipo 4 do vírus da hepatite C em Salvador, BA

Cited by 8 publications

References 9 publications

Prevalence of hepatitis C virus genotypes in the State of Pará, Brazil

Prevalence of hepatitis C virus genotypes in the State of Pará, Brazil

Distribution of Hepatitis C virus (HCV) genotypes in seropositive patients in the state of Alagoas, Brazil

One-step real-time PCR assay for detection and quantification of RNA HCV to monitor patients under treatment in Brazil

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