Detecção de riquétsias em carrapatos do gênero Amblyomma (Acari: Ixodidae) coletados em parque urbano do município de Campinas, SP

Estrada, Dora Amparo; Schumaker, Teresinha Tizu Sato; Souza, Celso Eduardo de; Neto, Elias José Rodrigues; Linhares, Arício Xavier

doi:10.1590/s0037-86822006000100013

Cited by 27 publications

(24 citation statements)

References 25 publications

(10 reference statements)

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“…In this regard lack of Rickettsia in the hemolymph of ticks from the reserve must be seen with some caution and cannot be regarded as a definitive result. Many false negative results may occur and even in spotted fever endemic areas R. rickettsii may be difficult to detect (Estrada et al 2006) thus further investigation in the reserve is warranted.…”

Section: Discussionmentioning

confidence: 99%

Ticks (Acari: Ixodidae) within various phytophysiognomies of a Cerrado reserve in Uberlândia, Minas Gerais, Brazil

et al. 2009

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Cerrado biome, the South American savannah, covers about 2 million km(2) and is very rich in endemic species but threatened by agriculture. In this report free-living tick species are presented, and their seasonal and relative distribution within the various phytophysiognomies in a small Cerrado reserve in Minas Gerais State, Brazil. Overall 2,694 free-living ticks were found during a 2 years sampling period with CO(2) traps and cloth dragging. Of these, 73.5% were Amblyomma cajennense and 0.6% Amblyomma dubitatum. All other ticks (25.9%) were retained as Amblyomma spp. Adults of A. cajennense peaked in spring, the nymphs in winter of both years. Amblyomma larval clusters were found in autumn and winter. Adult ticks (46.7%) and nymphs (39.5%) were most often found in woodlands, whereas most larval clusters were found in valley-side marshes (39%). Amblyomma cajennense, Anocentor nitens, Rhipicephalus (Boophilus) microplusand Rhipicephalus sanguineus ticks were found on domestic animals from neighboring properties. Search for Rickettsia in the hemolymph of 497 A. cajennense and one A. dubitatum ticks yielded negative results. Results confirmed earlier reports on the overwhelming prevalence of A. cajennense ticks in the Cerrado biome of Brazil and added information to habitat preferences of this tick species, a major vector in Brazil of the Rocky Mountain spotted fever.

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Section: Discussionmentioning

confidence: 99%

Ticks (Acari: Ixodidae) within various phytophysiognomies of a Cerrado reserve in Uberlândia, Minas Gerais, Brazil

et al. 2009

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“…Rickettsia bellii has already been identified in several species of ticks in Brazil (Estrada et al, 2006;Horta et al, 2007;Labruna et al, 2004aLabruna et al, , 2004bMcIntosh et al, 2015). In a Brazilian native people's reservation in the state of Mato Grosso, the circulation of R. bellii in Amblyomma sp.…”

Section: Discussionmentioning

confidence: 99%

Rickettsia spp., Ehrlichia spp. e Anaplasma spp. em carrapatos de vida livre nas mesorregiões Sul Fluminense e Metropolitana do Rio de Janeiro, RJ

Castro

Silveira

Ribeiro

et al. 2016

RBCV

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ResumoOs carrapatos são ectoparasitas obrigatórios e vetores de agentes patogênicos como bactérias, parasitos e vírus. O presente estudo teve como objetivo identificar a presença de bactérias dos gêneros Rickettsia, Ehrlichia e Anaplasma em carrapatos de vida livre coletados em cinco áreas, localizadas nas mesorregiões Sul Fluminense e Metropolitana do Rio de Janeiro. Foram coletados 9.353 carrapatos distribuídos em 372 pools. A análise dos dados pelo método PCR-RFLP indicou que as bandas verificadas na análise eram de Rickettsia bellii em 0,25% dos carrapatos coletados de duas áreas. A pesquisa molecular para os gêneros Ehrlichia e Anaplasma revelou resultados negativos para ambos os gêneros. A presença de genoma de R. bellii em carrapatos sugere a importância da execução de novas pesquisas com o intuito de entender a epidemiologia dos agentes estudados na região.Palavras-chave: ixodídeos, Rickettsia bellii, Rickettsiales. AbstractTicks are obligate ectoparasites and vectors of several bacterial, parasitic and viral pathogens. This study aimed to identify bacteria of the Rickettsia, Ehrlichia and Anaplasma genera in free-living ticks, collected in five areas located in both South Fluminense and Metropolitan mesoregions of Rio de Janeiro state. We collected a total of 9,353 ticks which were distributed in 372 pools. Data analysis using the PCR-RFLP method showed that the bands observed in the analysis are of the type Rickettsia bellii. The positivity level to R. bellii found in this study was 0.25%. The molecular search for Ehrlichia and Anaplasma genera revealed negative results for both genera. The presence of Rickettsiae DNA in the Rocky Mountain Spotted Fever group in ticks suggests the importance of undertaking new research in order to understand the epidemiology of the agents.

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“…As capybaras are the primary hosts for A. dubitatum and A. cajennense, an increased abundance of these species occurs in areas where these animals are established 36 . Reports of capybaras with positive serology for SFG rickettsiae 37 and reports of these animals with rickettsemia have been published.…”

Section: Epidemiological Questionnairementioning

confidence: 99%

Study of infection by Rickettsiae of the spotted fever group in humans and ticks in an urban park located in the City of Londrina, State of Paraná, Brazil

Toledo

Tamekuni

Filho

et al. 2011

Rev. Soc. Bras. Med. Trop.

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Introduction: Spotted fevers are emerging zoonoses caused by Rickettsia species in the spotted fever group (SFG). Rickettsia rickettsii is the main etiologic agent of Brazilian spotted fever (BSF) and it is transmitted by Amblyomma spp. ticks. Methods: The study aimed to investigate SFG rickettsiae in the Arthur Thomas Municipal Park in Londrina, PR, by collecting free-living ticks and ticks from capybaras and blood samples from personnel working in these areas. Samples from A. dubitatum and A. cajennense were submitted for PCR in pools to analyze the Rickettsia spp. gltA (citrate synthase gene). Results: All the pools analyzed were negative. Human sera were tested by indirect immunofluorescence assay with R. rickettsii and R. parkeri as antigens. Among the 34 sera analyzed, seven (20.6%) were reactive for R. rickettsii: four of these had endpoint titers equal to 64, 2 titers were 128 and 1 titer was 256. None of the samples were reactive for R. parkeri. An epidemiological questionnaire was applied to the park staff, but no statistically significant associations were identified. Conclusions: The serological studies suggest the presence of Rickettsiae related to SFG that could be infecting the human population studied; however, analysis of the ticks collected was unable to determine which species may be involved in transmission to humans.

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Detecção de riquétsias em carrapatos do gênero Amblyomma (Acari: Ixodidae) coletados em parque urbano do município de Campinas, SP

Cited by 27 publications

References 25 publications

Ticks (Acari: Ixodidae) within various phytophysiognomies of a Cerrado reserve in Uberlândia, Minas Gerais, Brazil

Ticks (Acari: Ixodidae) within various phytophysiognomies of a Cerrado reserve in Uberlândia, Minas Gerais, Brazil

Rickettsia spp., Ehrlichia spp. e Anaplasma spp. em carrapatos de vida livre nas mesorregiões Sul Fluminense e Metropolitana do Rio de Janeiro, RJ

Study of infection by Rickettsiae of the spotted fever group in humans and ticks in an urban park located in the City of Londrina, State of Paraná, Brazil

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