Detailed localization of aquaporin-4 messenger RNA in the CNS: preferential expression in periventricular organs

Venero, José L.; Vizuete, M.L.; Ilundáin, A.; Machado, Alberto; Echevarría, Miriam; Cano, Josefina

doi:10.1016/s0306-4522(99)00182-7

Cited by 120 publications

(96 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…On the contrary, AQP9-expressing cells lined the ventricles but showed a more pronounced subependymal-like distribution, with a clear presence in the dorsolateral part of the SVZ, which harbored many AQP9-positive cells coexpressing nestin and GFAP. Although not all the AQP 1 cells co-expressed GFAP, we did not find co-expression with neuronal markers, thus confirming the previously reported glial-restricted expression of AQP4 and AQP9 (Venero et al, 1999;. AQP4 expression in brain astrocytes has been correlated with the development, function and integrity of the BBB (Wen et Nicchia et al, 2004).…”

Section: Discussionsupporting

confidence: 79%

“…The abundance and specific cell distribution of AQP4 and AQP9 in our NSCs-derived cultures reflect the situation found in the adult rodent brain, with AQP4 being the most abundant water channel and AQP9 expression being restricted in a subset of astrocytes and in a different cellular compartment. The presence of AQP4 and AQP9 has not been described in cells of the oligodendroglial lineage, neither in vivo (Venero et al, 1999) nor in vitro Yoneda et al, 2001). Interestingly, we described the expression of both AQPs in a subset of oligodendrocytes, both in SVZ-derived primary cultures and NSC-derived differentiated cultures.…”

Section: Discussionmentioning

confidence: 99%

“…Progenitors displaying stem cell features have also been isolated from the fetal (Vescovi et al, 1999a,b;Svendsen et al, 1999;Carpenter et al, 1999) and adult (Sanai et al, 2004) human CNS. Both AQP4 and AQP9 expression is found in the adult forebrain periventricular region (Venero et al, 1999;Badaut et al, 2002), where the NSC pool resides. However, little is known about the expression of these proteins in the NSC population, either in vivo or in vitro.…”

Section: Introductionmentioning

confidence: 99%

“…Disregulation of water balance in the central nervous system (CNS) underlies focal edemas following brain tumors, ischemia, and traumatic injuries (King and Agre, 1996;Badaut et al, 2003). AQP4 and AQP9 are the most abundant brain AQPs (Venero et al, 1999;Saadoun et al, 2002). AQP4 is expressed in ependymal cells lining the ventricular system and subarachnoidal spaces as well as in glial endfeet contacting the blood-brain barrier (BBB), where it is thought to regulate water permeability and cerebrospinal fluid reabsorption (AmiryMoghaddam et al, 2003(AmiryMoghaddam et al, , 2004.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Unique expression and localization of aquaporin‐ 4 and aquaporin‐9 in murine and human neural stem cells and in their glial progeny

Cavazzin¹,

Ferrari²,

Facchetti

et al. 2005

Glia

View full text Add to dashboard Cite

Aquaporins (AQP) are water channel proteins that play important roles in the regulation of water homeostasis in physiological and pathological conditions. AQP4 and AQP9, the main aquaporin subtypes in the brain, are expressed in the adult forebrain subventricular zone (SVZ), where neural stem cells (NSCs) reside, but little is known about their expression and role in the NSC population, either in vivo or in vitro. Also, no reports are available on the presence of these proteins in human NSCs. We performed a detailed molecular and phenotypical characterization of different AQPs, and particularly AQP4 and AQP9, in murine and human NSC cultures at predetermined stages of differentiation. We demonstrated that AQP4 and AQP9 are expressed in adult murine SVZ-derived NSCs (ANSCs) and that their levels of expression and cellular localization are differentially regulated upon ANSC differentiation into neurons and glia. AQP4 (but not AQP9) is expressed in human NSCs and their progeny. The presence of AQP4 and AQP9 in different subsets of ANSC-derived glial cells and in different cellular compartments suggests different roles of the two proteins in these cells, indicating that ANSC-derived astrocytes might maintain in vitro the heterogeneity that characterize the astrocyte-like cell populations in the SVZ in vivo. The development of therapeutic strategies based on modulation of AQP function relies on a better knowledge of the functional role of these channels in brain cells. We provide a reliable and standardized in vitro experimental model to perform functional studies as well as toxicological and pharmacological screenings. V V C 2005 Wiley-Liss, Inc.

show abstract

Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Unique expression and localization of aquaporin‐ 4 and aquaporin‐9 in murine and human neural stem cells and in their glial progeny

Cavazzin¹,

Ferrari²,

Facchetti

et al. 2005

Glia

View full text Add to dashboard Cite

show abstract

“…AQP1 is abundant on the apical (CSF-facing) surface of rat CPs from the fourth and lateral ventricles (Nielsen et al 1993;Speake et al 2003), therefore during the CSF secretion it plays a major role in mediating water transport across the apical membrane (Oshio et al 2005). The mRNA for AQP4 was initially detected in rat CPs from lateral ventricles, using the hybridization in situ method (Venero et al 1999), thereafter also AQP4 protein was detected as diffusely distributed throughout the epithelial cells of rat CPs from the fourth and lateral ventricles (Speake et al 2003;Nazari et al 2015). It has been demonstrated that in rats the expression of AQP4 protein in astrocytes and AQP1 protein in spinal cord may be modulated by melatonin (Kaur et al 2006;Nesic et al 2008).…”

Section: Doi: 1017221/6/2017-cjasmentioning

confidence: 99%

Effect of melatonin from slow-release implants on aquaporins (AQP1 and AQP4) in the ovine choroid plexus

Szczepkowska¹,

Skowroński²,

Kowalewska³

et al. 2018

CZECH J ANIM SCI

View full text Add to dashboard Cite

Szczepkowska A., Skowroński M.T., Kowalewska M., Milewski S., Skipor J. (2018): Effect of melatonin from slow-release implants on aquaporins (AQP1 and AQP4) in the ovine choroid plexus. Czech J. Anim. Sci., 63, 32-42.Aquaporins (AQPs) play important role in the cerebrospinal fluid (CSF) secretion and AQP1 and AQP4 are localized in the choroid plexus (CP), which is the main place of CSF production. In ewes, seasonally breeding animals, the turnover rate (TOR) of CSF is photoperiodically modulated and melatonin, a biochemical signal about changing photoperiod, is used to advance the onset of the breeding season by mimicking the stimulatory effect of short days (SD). This study evaluates the effect of melatonin implantation during long days (LD) on AQPs expression in the ovine CP. Studies were performed on ovariectomized, estradiol implanted ewes treated with placebo (n = 6) or with melatonin (n = 6) during LD. Ewes were sacrificed 40 days after melatonin/ placebo implantation and CPs from the lateral/third brain ventricles were collected for Real-time and Western blot analyses. Additionally, for immunohistochemical analysis, CP samples were collected from ewes (n = 3) sacrificed during LD. We demonstrated an apical membrane localization of AQP1 and a diffused distribution of AQP4 in the epithelial cells of CP. The mRNA expression of AQP1 was 20 times higher than the expression of all AQP4 isoforms, and among them AQP4 isoforms containing exon 2 constituted approximately 38%. The melatonin implantation significantly (P < 0.05) increased the mRNA expression of AQP1 and AQP4 and the protein level of AQP4 isoforms (33 and 28 kDa). For AQP1 we observed a significant (P < 0.05) decrease of glycosylated (33 kDa) and a significant (P < 0.05) increase of unglycosylated (23 kDa) predominant protein form. Therefore it can be suggested that at least AQP1, which is involved in CSF production and has been demonstrated to be modulated by melatonin implantation, is linked with the photoperiodic modulation of the CSF production in ewes.

show abstract

Neuromyelitis Optica Brain Lesions Localized at Sites of High Aquaporin 4 Expression

Pittock¹,

Weinshenker²,

Lucchinetti³

et al. 2006

Arch Neurol

641

450

View full text Add to dashboard Cite

Background: Neuromyelitis optica (NMO)-IgG is a specific autoantibody marker for NMO. It binds selectively to aquaporin 4 (AQP4), which is highly concentrated in astrocytic foot processes at the blood-brain barrier and is not restricted to optic nerve and spinal cord. Although it is conventionally believed that the brain is spared, brain imaging abnormalities are not uncommon in patients with NMO.Objective: To investigate the location of brain lesions that are distinctive for NMO with respect to the localization of AQP4 in mammalian brain.Design: Observational, retrospective case series. Setting:Clinical serologic cohort of patients tested for NMO-IgG for whom brain MRI images were available. Patients:We identified 120 patients seropositive for NMO-IgG for whom brain magnetic resonance images were available. Main Outcome Measure: Magnetic resonance imaging abnormalities.Results: In 8 patients we observed recurring and distinctive magnetic resonance imaging abnormalities in the hypothalamic and periventricular areas that corresponded to brain regions of high AQP4 expression. Conclusion:The distribution of NMO-characteristic brain lesions corresponds to sites of high AQP4 expression.

show abstract

Detailed localization of aquaporin-4 messenger RNA in the CNS: preferential expression in periventricular organs

Cited by 120 publications

References 26 publications

Unique expression and localization of aquaporin‐ 4 and aquaporin‐9 in murine and human neural stem cells and in their glial progeny

Unique expression and localization of aquaporin‐ 4 and aquaporin‐9 in murine and human neural stem cells and in their glial progeny

Effect of melatonin from slow-release implants on aquaporins (AQP1 and AQP4) in the ovine choroid plexus

Neuromyelitis Optica Brain Lesions Localized at Sites of High Aquaporin 4 Expression

Contact Info

Product

Resources

About