Detailed investigation of the diagnostic value in tumour histopathology of ICR.2, a new monoclonal antibody to epithelial membrane antigen

Imrie, S F; Sloane, J P; Ormerod, M.G.; Styles, J M; Dean, Christopher

doi:10.1111/j.1365-2559.1990.tb01162.x

Cited by 9 publications

(8 citation statements)

References 22 publications

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“…The rat IgG2a MAb ICR2 (Imrie et al, 1990) was used in the present study. This antibody was raised in Chester Beattie Hooded rats using defatted human milk fat globule membrane as immunogen.…”

Section: Radiolabelled Antibodymentioning

confidence: 99%

The effect of circulating antigen and radiolabel stability on the biodistribution of an indium labelled antibody

Davidson

Babich²,

Young³

et al. 1991

Br J Cancer

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Summary This study has investigated two of the main problems with radiolabelled antibody imaging, the formation of circulating immune complexes (I.C.) and the non specific binding of radiolabel to the antibody molecule.Patients undergoing immunoscintigraphy with "'In labelled monoclonal antibody ICR2 were divided into three groups who received either the radiolabelled antibody alone (control, n = 12), the radiolabelled antibody which was incubated with the chelating agent diethylene triamine pentacetic acid (DTPA) prior to size exclusion chromatography (n = 6) or whose injectate was treated with DTPA and cold MAb administered intravenously prior to radiolabelled MAb administration (n = 6). Radiolabelled antibody uptake in abdominal organs was measured by region of interest analysis using a gamma camera with online computer and that in tumour and normal tissues by gamma well counting of biopsies. Circulating antigen and immune complex was measured by high pressure liquid chromatography (HPLC).The sensitivity of tumour imaging and the tumour uptake of radiolabelled antibody was not significantly different between the groups. Patients with high circulating antigen levels developed high levels of circulating immune complex but also had high tumour uptakes of radiolabelled antibody. Administration of cold MAb increased the splenic, but did not effect the tumour uptake of radiolabelled antibody and only minimally reduced levels of circulating immune complex. Chelate administration reduced the urinary excretion of radioactivity but increased the liver uptake of radioactivity.These results have shown that successful antibody imaging can be carried out despite high levels of circulating antigen, that large doses of unlabelled antibody are required to prevent immune complex formation and that removal of non specifically bound "'In does not reduce the liver uptake of radioactivity.

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Section: Radiolabelled Antibodymentioning

confidence: 99%

The effect of circulating antigen and radiolabel stability on the biodistribution of an indium labelled antibody

Davidson

Babich²,

Young³

et al. 1991

Br J Cancer

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“…Antibodies to the epithelial membrane antigen (EMA) [20,21,381 have been used to label luminal cells, and the common acute lymphoblastic leukemia antigen (CALLA/CDl O/neutral endopeptidase 24.1 1) was used to mark the myoepithelial cells [17]. We report here the appearaucc.…”

Section: Introductionmentioning

confidence: 99%

Characterization in vitro of luminal and myoepithelial cells isolated from the human mammary gland by cell sorting

et al. 1991

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“…The total absence of skin involvement and the partial clear cell pattern speaks against spindle-cell melanoma [3]. Besides the identification by the protein S100 [1,3] other new techniques are now used to confirm the diagnosis of CCS such as ICR2 [5], a new monoclonal antibody to epithelial membrane antigen and HMB-45 [4] a melaninassociated antigen. On the other hand, cytogenetic techniques [2] are reported to demonstrate clonal, numerical and structural abnormalities with specific involvement of chromosome 2 and 22 in CCS.…”

Section: Pathologic Findingsmentioning

confidence: 99%

“…The presence of lymph node metastasis and intracellular glycogen speaks in favor of malignant melanoma. However, the presence of melanocytes in tendons and aponeurosis has never been recognized [5]. The positive reaction with PS 100 is additional proof in favor of malignant melanoma and clear cell sarcoma.…”

Section: Pathologic Findingsmentioning

confidence: 99%

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An Unusual Presentation of Clear Cell Sarcoma

Khoury¹,

Bouzo²,

Tabbara³

1992

Ann Saudi Med

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A 34-year-old left-handed British male was referred with a slightly painful "recurrent ganglion" over the dorsum of the right wrist of six months' duration. One month prior to presentation the patient noticed the appearance of another small lump over the dorsum of the right hand which was asymptomatic. The "ganglion" was curetted ten months prior to admission in an outside clinic under local anesthesia. There was no report of the original specimen being sent for pathology.The patient underwent exploration and excision of both masses under regional block with a double tourniquet (Biers block). The dorsal wrist mass was found to originate from the sheath of the extensor carpi radialis brevis and adhering to the extensor retinaculum and dorsal capsule of the wrist. The dorsal wrist synovium was intact. The small mass over the dorsum of the right hand was originating from the sheath of the extensor tendon of the ring finger. Both were excised completely. Pathologic FindingsThe material presented consisted of: (1) an irregular piece of white rubbery tissue measuring 1.0 × 0.5 × 0.3 cm, all blocked; (2) a globular mass of pale gray rubbery tissue measuring 3.0 × 2.0 × 1.2 cm with a small segment of tendon tissue. On cut surface the mass was adherent to the tendon and was composed of pale gray soft tissue with areas of hemorrhage. Microscopically, the tumor was composed of nests and fascicles of polygonal rounded or spindle-shaped cells (Figure 1). They presented vesicular nuclei with very prominent single central nucleolei. The cytoplasm was abundant and often clear. Mitoses were frequent, climaxing to 4 per 10 high-power field, with an occasional atypical figure (Figure 2). In a few scattered fields there were multinucleated giant cells. Mucin and alcian blue stains were negative. Periodic acid-Schiff (PAS) stain gave a faintly positive response. Silver impregnation demonstrated a loose network of reticulin fibers that surrounded the tumor cells individually and in small nests. Fontana and iron stains did not react with tumor cells. Inconspicuous iron deposits were noted in the interstitium. The absence of demonstrable melanin in sections stained by the Fontana technique does not rule out the diagnosis of clear cell sarcoma (CCS) of the tendon sheath because melanin is demonstrable in only 50% of the cases [1,2].This tumor is readily differentiated from synovial sarcoma because of the absence of biphasic cellular pattern and intracellular mucin formation. The presence of clear cells and the nesting of the tumor cells on silver impregnation speak against a fibrosarcoma. The differentiation of epithelioid malignant schwannoma or a spindlecell melanoma is more difficult. The absence of peripheral nerves within the vicinity and of any history of neurofibromatosis are arguments against the former. The total absence of skin involvement and the partial clear cell pattern speaks against spindle-cell melanoma [3].

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Detailed investigation of the diagnostic value in tumour histopathology of ICR.2, a new monoclonal antibody to epithelial membrane antigen

Cited by 9 publications

References 22 publications

The effect of circulating antigen and radiolabel stability on the biodistribution of an indium labelled antibody

The effect of circulating antigen and radiolabel stability on the biodistribution of an indium labelled antibody

Characterization in vitro of luminal and myoepithelial cells isolated from the human mammary gland by cell sorting

An Unusual Presentation of Clear Cell Sarcoma

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