Detailed Hylobates lar karyotype defined by 25-color FISH and multicolor banding

Mrasek, Kristin; Heller, Anita; Рубцов, Н. М.; Трифонов, Владимир; Starke, Heike; Claussen, Uwe; Liehr, Thomas

doi:10.3892/ijmm.12.2.139

Cited by 28 publications

(44 citation statements)

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“…Additionally, we showed the suitability of mMCB for ZOO-FISH studies, which were done by chromosome-specific MCB previously in GGO (Mrasek et al, 2001) and Hylobates lar (Mrasek et al, 2003). For such studies mMCB is time-saving, too.…”

Section: Discussionmentioning

confidence: 99%

Multitude multicolor chromosome banding (mMCB) – a comprehensive one-step multicolor FISH banding method

Weise

Heller

Starke

et al. 2003

Cytogenet Genome Res

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Multicolor chromosome banding (MCB) using one single chromosome-specific MCB probe set per experiment was previously reported as powerful tool in molecular cytogenetics for the characterization of all kinds of human marker chromosomes. However, a quick analysis of karyotypes with highly complex chromosomal changes was hampered by the problem that up to 24 MCB experiments were necessary for a comprehensive karyotype description. To overcome that limitation the 138 available region-specific microdissection-derived libraries for all human chromosomes were combined to one single probe set, called multitude MCB (mMCB). A typical fluorescence banding pattern along the human karyotype is produced, which can be evaluated either by transforming these profiles into chromosome region-specific pseudo-colors or more reliably by studying the fluorescence profiles. The mMCB probe set has been applied on chromosomes of normal male and female probands, two primary myelodysplastic syndromes and two solid tumor cell lines. Additionally, a cell line of Gorilla gorilla (GGO) studied previously by single chromosome-specific MCB was reevaluated by the mMCB method. All results were in concordance with those obtained in parallel or by other cytogenetic and molecular cytogenetic approaches indicating that mMCB is a powerful multicolor FISH banding tool for fast characterization of complex karyotypes.

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Section: Discussionmentioning

confidence: 99%

Multitude multicolor chromosome banding (mMCB) – a comprehensive one-step multicolor FISH banding method

Weise

Heller

Starke

et al. 2003

Cytogenet Genome Res

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“…Each genus has a different diploid number: Hoolock, 2n = 38; Hylobates, 2n = 44; Symphalangus, 2n = 50; and Nomascus, 2n = 52. Chromosome translocations in each karyomorph have been documented using human and cross-species painting probes (Jauch et al 1992;Koehler et al 1995a,b;Arnold et al 1996;Nie et al 2001;Muller et al 2002Muller et al , 2003Hirai et al 2003;Mrasek et al 2003;Ferguson-Smith et al 2005). Recently, the chromosomes of the white-cheeked crested gibbon (Nomascus leucogenys, NLE) were defined in greater detail using highresolution methods Roberto et al 2007).…”

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confidence: 99%

Tracking the complex flow of chromosome rearrangements from the Hominoidea Ancestor to extant Hylobates and Nomascus Gibbons by high-resolution synteny mapping

Misceo¹,

Capozzi²,

Roberto³

et al. 2008

Genome Res.

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In this study we characterized the extension, reciprocal arrangement, and orientation of syntenic chromosomal segments in the lar gibbon (Hylobates lar, HLA) by hybridization of a panel of ∼1000 human BAC clones. Each lar gibbon rearrangement was defined by a splitting BAC clone or by two overlapping clones flanking the breakpoint. A reconstruction of the synteny arrangement of the last common ancestor of all living lesser apes was made by combining these data with previous results in Nomascus leucogenys, Hoolock hoolock, and Symphalangus syndactylus. The definition of the ancestral synteny organization facilitated tracking the cascade of chromosomal changes from the Hominoidea ancestor to the present day karyotype of Hylobates and Nomascus. Each chromosomal rearrangement could be placed within an approximate phylogenetic and temporal framework. We identified 12 lar-specific rearrangements and five previously undescribed rearrangements that occurred in the Hylobatidae ancestor.

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“…However, the HCM-FISH probe set could also be used to answer questions in other fields, such as tumor cytogenetics or evolutionary studies. Examples would be interstitial heterochromatin in tumor-associated derivative chromosomes (Doneda et al 1989) or studies on evolutionarily conserved heterochromatin (Mrasek et al 2003). If heterochromatic material is attached to the tip of a nonacrocentric chromosomal arm, the carrier can be clinically normal and only detected due to infertility or clinically affected due to essential loss of subtelomeric material in the "receiving" chromosome.…”

Section: Discussionmentioning

confidence: 99%

“…Midi was done as previously reported (Liehr et al 2002b). Midi probes for the regions 1q12, 9q12, 15p12~11.2 (i.e., a β-satellite-specific probe), 16q11.2, 19p12~19q12, and Yq12 were established for this probe set, while those probes for 9p12/9q13 (midi 36) and for all acrocentric short arms (midi 54) were as previously reported (Mrasek et al 2003).…”

Section: Hcm-fish Probe Setmentioning

confidence: 99%

A New Multicolor Fluorescence In Situ Hybridization Probe Set Directed Against Human Heterochromatin

Bucksch

Ziegler

Kosayakova

et al. 2012

J Histochem Cytochem.

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SummaryA new multicolor fluorescence in situ hybridization (mFISH) probe set is presented, and its possible applications are highlighted in 25 clinical cases. The so-called heterochromatin-M-FISH (HCM-FISH) probe set enables a one-step characterization of the large heterochromatic regions within the human genome. HCM-FISH closes a gap in the now available mFISH probe sets, as those do not normally cover the acrocentric short arms; the large pericentric regions of chromosomes 1, 9, and 16; as well as the band Yq12. Still, these regions can be involved in different kinds of chromosomal rearrangements such as translocations, insertions, inversions, amplifications, and marker chromosome formations. Here, examples are given for all these kinds of chromosomal aberrations, detected as constitutional rearrangements in clinical cases. Application perspectives of the probe set in tumors as well as in evolutionary cytogenetic studies are given. (J Histochem Cytochem 60:530-536, 2012) Keywords multicolor fluorescence in situ hybridization (mFISH), heterochromatin-M-FISH (HCM-FISH) probe set, heteromorphism, small supernumerary marker chromosome (sSMC), insertion, translocation Article

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Detailed Hylobates lar karyotype defined by 25-color FISH and multicolor banding

Cited by 28 publications

References 0 publications

Multitude multicolor chromosome banding (mMCB) – a comprehensive one-step multicolor FISH banding method

Multitude multicolor chromosome banding (mMCB) – a comprehensive one-step multicolor FISH banding method

Tracking the complex flow of chromosome rearrangements from the Hominoidea Ancestor to extant Hylobates and Nomascus Gibbons by high-resolution synteny mapping

A New Multicolor Fluorescence In Situ Hybridization Probe Set Directed Against Human Heterochromatin

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