Detailed description of four YAC contigs representing 17 Mb of chromosome 4 of <i>Arabidopsis thaliana</i> ecotype Columbia

Schmidt, Renate; West, Joanne; Cnops, Gerda; Love, Karina; Balestrazzi, Alma; Dean, Caroline

doi:10.1046/j.1365-313x.1996.9050755.x

Cited by 50 publications

(38 citation statements)

References 21 publications

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“…On average, 8.7 YAC clones were isolated for each of the chromosome 5 loci; this figure is almost identical to that found for chromosome 4 (8.5 YAC clones per locus, Schmidt et al, 1996) using the same approach. Some genomic regions were poorly covered by YAC clones, for example only one YAC clone hybridizing to marker m248 was found in all four libraries.…”

Section: Discussionsupporting

confidence: 79%

“…Southern blot analysis of YAC clones using markers as probes in combination with the size information allowed us to position the YAC clones relative to each other and to the markers in the YAC contigs. The rules governing how the contigs have been drawn have been described previously (Schmidt et aL, 1996).…”

Section: Alignment Of Yac Clones Relative To Markers In the Yac Contigsmentioning

confidence: 99%

“…Southern blot analyses using the repetitive sequences as probes confirmed that 77 YAC clones carried chromosome 5-specific sequences in addition to unlinked repetitive sequences (Table 1). Second, all data obtained with the marker hybridization experiments were analysed to show whether YAC clones hybridized to unlinked locations on chromosome 5 or whether YAC clones carried chromosome 4- (Schmidt et aL, 1996) as well as chromosome 5-specific sequences. Ten YAC clones hybridized to two different locations on chromosome 5 and 18 clones were found to contain chromosome 4-as well as chromosome 5-specific sequences.…”

Section: Chimaeric Yac Clonesmentioning

confidence: 99%

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Description of 31 YAC contigs spanning the majority of Arabidopsis thaliana chromosome 5

Schmidt¹,

Love

West

et al. 1997

The Plant Journal

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Section: Discussionsupporting

confidence: 79%

Section: Alignment Of Yac Clones Relative To Markers In the Yac Contigsmentioning

confidence: 99%

Section: Chimaeric Yac Clonesmentioning

confidence: 99%

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Description of 31 YAC contigs spanning the majority of Arabidopsis thaliana chromosome 5

Schmidt¹,

Love

West

et al. 1997

The Plant Journal

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“…Ends of the YAC inserts were amplified by inverted PCR (IPCR, ref. 31), subcloned in pGEM-T and sequenced. YAC end probes were either used directly for RFLP mapping or were converted into CAPS markers before mapping.…”

Section: Rapid Amplification Of Cdna Ends (Race)mentioning

confidence: 99%

The tomato Blind gene encodes a MYB transcription factor that controls the formation of lateral meristems

Schmitz

Tillmann

Carriero

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

248

223

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The multitude of forms observed in flowering plants is largely because of their ability to establish new axes of growth during postembryonic development. This process is initiated by the formation of secondary meristems that develop into vegetative or reproductive branches. In the blind and torosa mutants of tomato, initiation of lateral meristems is blocked during shoot and inflorescence development, leading to a strong reduction in the number of lateral axes. In this study, it is shown that blind and torosa are allelic. The Blind gene has been isolated by positional cloning, and it was found that the mutant phenotype is caused by a loss of function of an R2R3 class Myb gene. RNA interference-induced blind phenocopies confirmed the identity of the isolated gene. Double mutant analysis shows that Blind acts in a novel pathway different from the one to which the previously identified Lateral suppressor gene belongs. The findings reported add a new class of transcription factors to the group of genes controlling lateral meristem initiation and reveal a previously uncharacterized function of R2R3 Myb genes.I n flowering plants, postembryonic shoot development is controlled by the activity of the shoot apical meristem (SAM). The SAM established during embryogenesis produces in a regular fashion leaf, node, and internode primordia, which generate the primary shoot of a plant. Secondary meristems arise in the axils of leaves as well as on the flanks of the inflorescence meristems. Lateral meristems produced during the vegetative phase develop into shoots repeating, at least in part, the growth pattern of the primary shoot, whereas after floral transition lateral meristems develop into flowers or new inflorescence axes.In Arabidopsis thaliana, Zea mays, and several other plant species, genes have been isolated that are required for SAM initiation, maintenance, and function, and interactions between these genes are being studied (1). However, much less is known about the genetic control of lateral meristem initiation and function during shoot and inflorescence development. In tomato, lateral meristems are formed in all leaf axils and are first detectable in the axil of the fifth youngest primordium (2). They develop into fast-growing side shoots that give the plant a bushy appearance. Whereas in many higher plants the primary SAM remains active throughout the entire lifespan, in tomato, it is transformed into a terminal inflorescence, and the uppermost axillary meristem takes over its function to continue the main stem. After formation of three leaves, this sympodial shoot terminates itself in an inflorescence, and sympodial shoots of progressively higher order elongate the main axis (3, 4). The tomato inf lorescence has been described as a cyme, i.e., flowers arise as terminal structures, and the inflorescence axis grows because of a lateral meristem, which will again be transformed into a floral meristem and so on (3). Recent analysis has in part modified this view by demonstrating that the inflorescence meristem is s...

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“…Recent developments in Arabidopsis genomics research include the construction of a nearly complete physical map of the genome based on yeast artificial chromosomes (Choi et al, 1995;Schmidt et al, 1996Schmidt et al, , 1997Zachgo et al, 1996;Camilleri et al, 1998), bacterial artificial chromosomes (BACs; Mozo et al, 1998), P1 clones (Liu et al, 1995;Kaneko et al, 1998), the systematic sequencing of expressed sequence tags (ESTs; Höfte et al, 1993;Newman et al, 1994), and coordinated multinational sequencing efforts (Goodman et al, 1995;Bevan et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

A Two-Component Enhancer-Inhibitor Transposon Mutagenesis System for Functional Analysis of the Arabidopsis Genome

et al. 1999

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A modified Enhancer-Inhibitor transposon system was used to generate a series of mutant lines by single-seed descent such that multiple I insertions occurred per plant. The distribution of original insertions in the population was assessed by isolating transposon-flanking DNA, and a database of insertion sites was created. Approximately threequarters of the identified insertion sites show similarity to sequences stored in public databases, which demonstrates the power of this regimen of insertional mutagenesis. To isolate insertions in specific genes, we developed threedimensional pooling and polymerase chain reaction strategies that we then validated by identifying mutants for the regulator genes APETALA1 and SHOOT MERISTEMLESS. The system then was used to identify inserts in a class of uncharacterized genes involved in lipid biosynthesis; one such insertion conferred a fiddlehead mutant phenotype. INTRODUCTIONOver the past two decades, Arabidopsis has become a model organism for research in plant physiology, development, biochemistry, and pathogenesis. Recent developments in Arabidopsis genomics research include the construction of a nearly complete physical map of the genome based on yeast artificial chromosomes (Choi et al., 1995;Schmidt et al., 1996Schmidt et al., , 1997 Zachgo et al., 1996;Camilleri et al., 1998), bacterial artificial chromosomes (BACs; Mozo et al., 1998), P1 clones (Liu et al., 1995Kaneko et al., 1998), the systematic sequencing of expressed sequence tags (ESTs; Höfte et al., 1993;Newman et al., 1994), and coordinated multinational sequencing efforts (Goodman et al., 1995;Bevan et al., 1998).The large amount of sequence information generated by Arabidopsis genome and EST sequencing projects will provide the basis for systematic studies of gene function and eventually will allow for unlimited comparisons with the yeast (Mewes et al., 1997) and Caenorhabditis ( C. elegans Sequencing Consortium, 1998) genomes. Such cross-kingdom analyses might reveal those genes that are necessary for the development and maintenance of eukaryotic cells and those that are associated with functions unique to either plants or animals. An intriguing finding to have emerged already from these studies is that half of the Arabidopsis ESTs bear no significant similarity to sequences from the previously established databases (Rounsley et al., 1996).Over the past decade, insertional mutagenesis has proven to be one of the most efficient ways of isolating and identifying genes via the traditional approaches of "forward" genetics (Feldmann, 1991;Koncz et al., 1992;Aarts et al., 1993;Jones et al., 1994;Okuley et al., 1994;Azpiroz-Leehan and Feldmann, 1997). In light of the ongoing accumulation of ever more DNA sequences of unknown function, insertional mutagenesis now offers the means for determining the function of newly elaborated sequences by a process of "reverse" genetics. The strategy begins with the broad insertional mutagenesis of the genome through the use of T-DNA or transposons followed by the identification o...

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Detailed description of four YAC contigs representing 17 Mb of chromosome 4 of Arabidopsis thaliana ecotype Columbia

Cited by 50 publications

References 21 publications

Description of 31 YAC contigs spanning the majority of Arabidopsis thaliana chromosome 5

Description of 31 YAC contigs spanning the majority of Arabidopsis thaliana chromosome 5

The tomato Blind gene encodes a MYB transcription factor that controls the formation of lateral meristems

A Two-Component Enhancer-Inhibitor Transposon Mutagenesis System for Functional Analysis of the Arabidopsis Genome

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