Detailed clinicopathological characteristics and possible lymphomagenesis of type II intestinal enteropathy-associated T-cell lymphoma in Japan

High Expression of Intestinal Homing Receptor CD103 in Adult T-Cell Leukemia/Lymphoma, Similar to 2 Other CD8+ T-Cell Lymphomas

“…20,21 Type II EATL mainly invades the small intestine and occasionally the other GI sections. 22 Cases of intestinal EBER + T/NK-cell lymphoma showed the rare histologic features of type II EATL described above and rare gastric invasion. 21,23 To our knowledge, this is the first demonstration that GI-involved ATLL has high CD103 expression and specific histologic features similar to those of EATL.…”

“…Further, CD4 + /CD5 + T-IELs and CD4 + /CD25 + mucosal T cells are found in the gastric mucosa, but occasionally in the intestinal mucosa. 22,23,28,29 ATLL phenotypes have been suggested to have characteristics more similar to those of gastric intramucosal T cells than to intestinal T cells.…”

Clinicopathological characteristics and rituximab addition to cytotoxic therapies in patients with rheumatoid arthritis and methotrexate‐associated large B lymphoproliferative disorders

“…Immunohistological examination was performed by applying a panel of monoclonal and polyclonal antibodies to formalin‐fixed tumour samples using the ChemMate Envision method (DakoCytomation, Glostrup, Denmark). The peroxidase colour reaction was developed using diaminobenzidine (DAB) as the substrate . Antibody, dilution, clone and source are as follows: CD3 (1:100, PS1; Leica, Newcastle upon Tyne, UK), CD10 (1:100, 56C6; Novocastra, Newcastle upon Tyne, UK), CD20 (1:100, L26; Nichirei, Tokyo, Japan), multiple myeloma 1 (1:100, MUM1; DakoCytomation), CD25 (1:200, 4C9; Leica), CD15 (1:100, LeuM1; DakoCytomation), CD30 (1:50, BerH2; DakoCytomation), BCL2 (1:500, 124; DakoCytomation), LMP1 (1:200, CS1‐4; DakoCytomation), EBNA2 (1:200; Leica) and ZEBRA (1:200, BZ.1; DakoCytomation).…”

“…The peroxidase colour reaction was developed using diaminobenzidine (DAB) as the substrate. 14 Tumour cell counts were calculated semiquantitatively by detection of CD20 in 26-50%, 51-75% and more than 76% of cells. Necrosis was estimated as negative, focal (fewer than 10%) and massive (more than 10%) of cells.…”

Enteropathy-associated T cell lymphoma subtypes are characterized by loss of function of SETD2

“…Type I EATL has a more variable histology, is more prevalent in Northern Europe (Delabie et al, 2011), and is strongly associated with celiac disease. Type II EATL has a more uniform histology, occasional association with celiac disease (Delabie et al, 2011), and is more prevalent in Asia (Sun et al, 2011; Tse et al, 2012; Kikuma et al, 2014). The diagnostic distinction between these two types is not always clear.…”