Detailed analysis of the turnover of polyphosphoinositides and phosphatidic acid upon activation of phospholipases C and D in Chlamydomonas cells treated with non-permeabilizing concentrations of mastoparan

Munnik, Teun; Himbergen, J.A.J. van; Riet, Bas ter; Braun, Franz-Josef; Irvine, Robin F.; Ende, H. van den; Musgrave, A.

doi:10.1007/s004250050465

Cited by 110 publications

(167 citation statements)

References 60 publications

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“…Cabbage PLD (1 U; type V, Sigma-Aldrich, Steinheim, Germany) was used as a positive control. Lipids were extracted as described before (van der Luit et al, 2000) and separated on silica TLC plates using the organic upper phase of an ethyl acetate mixture: ethyl acetate/isooctane/formic acid/water (12 : 2 : 3 : 10 [v/v]; Munnik et al, 1998). BODIPY-lipids were visualized under UV light and quantified by fluoroimaging (Molecular Dynamics, Sunnyvale CA, USA).…”

Section: Tomato Plant and Cell-suspension Transformationmentioning

confidence: 99%

“…For quantitative analysis, lipids were separated on silica TLC plates using the organic upper phase of an ethyl acetate mixture: ethyl acetate/iso-octane/formic acid/water (12 : 2 : 3 : 10 [v/v]; Munnik et al, 1998) O (80 : 30 : 28 : 26 : 15 [v/v]) in the second dimension. Phospholipids were visualized by autoradiography and quantified by phosphoimaging (Molecular Dynamics, Sunnyvale CA, USA).…”

Section: Tomato Plant and Cell-suspension Transformationmentioning

confidence: 99%

“…The remainder of the extraction was performed as described before (van der Luit et al, 2000). Lipids were separated on TLC plates using the organic upper phase of an ethyl acetate mixture: ethyl acetate/iso-octane/formic acid/water (12 : 2 : 3 : 10 [v/v]; Munnik et al, 1998) Munnik et al, 1994) when indicated. Radiolabeled phopspholipids were visualized by autoradiography and quantified by phosphoimaging (Molecular Dynamics, Sunnyvale CA, USA).…”

Section: Rna Blot Analysismentioning

confidence: 99%

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The role of phospholipase D in plant stress responses

Bargmann

Munnik

2006

Current Opinion in Plant Biology

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Section: Tomato Plant and Cell-suspension Transformationmentioning

confidence: 99%

Section: Tomato Plant and Cell-suspension Transformationmentioning

confidence: 99%

Section: Rna Blot Analysismentioning

confidence: 99%

See 1 more Smart Citation

The role of phospholipase D in plant stress responses

Bargmann

Munnik

2006

Current Opinion in Plant Biology

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284

183

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“…1, A, C, E, and G) but could have activated the PLC pathway as well. To test whether PLC and DGK are activated by these treatments, a differential labeling strategy was used (Munnik et al, 1998b). The method is based on the principle that radioactive orthophosphate is slowly incorporated into structural lipids, but much faster into the ATP pool.…”

Section: Plc In Combination With Dgk Contributes To Pa Formationmentioning

confidence: 99%

Nod Factor and Elicitors Activate Different Phospholipid Signaling Pathways in Suspension-Cultured Alfalfa Cells

2003

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Lipo-chitooligosaccharides (Nod factors) are produced by symbiotic Rhizobium sp. bacteria to elicit Nod responses on their legume hosts. One of the earliest responses is the formation of phosphatidic acid (PA), a novel second messenger in plant cells. Remarkably, pathogens have also been reported to trigger the formation of PA in nonlegume plants. To investigate how host plants can distinguish between symbionts and pathogens, the effects of Nod factor and elicitors (chitotetraose and xylanase) on the formation of PA were investigated in suspension-cultured alfalfa (Medicago sativa) cells. Theoretically, PA can be synthesized via two signaling pathways, i.e. via phospholipase D (PLD) and via phospholipase C in combination with diacylglycerol (DAG) kinase. Therefore, a strategy involving differential radiolabeling with [ 32 P]orthophosphate was used to determine the contribution of each pathway to PA formation. In support, PLD activity was specifically measured by using the ability of the enzyme to transfer the phosphatidyl group of its substrate to a primary alcohol. In practice, Nod factor, chitotetraose, and xylanase induced the formation of PA and its phosphorylated product DAG pyrophosphate within 2 min of treatment. However, whereas phospholipase C and DAG kinase were activated during treatment with all three different compounds, PLD was only activated by Nod factor. No evidence was obtained for the activation of phospholipase A 2 .

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“…More speci®cally, it is thought to play a role in the response to pathogens (Chapman, 1998;Young et al, 1996), wounding (Lee et al, 1997;Ryu and Wang, 1998), water stress (Frank et al 2000;Munnik et al, unpublished data), and the plant stress hormones ethylene (Fan et al, 1997;Lee et al, 1998) and abscisic acid (Fan et al, 1997;Jacob et al, 1999;Ritchie and Gilroy, 1998). Some of these effects may be mediated by G-protein-coupled stress receptors, because G-protein activators such as mastoparans, alcohols and cholera toxin activate PLD in the absence of stress (De Vrije and Munnik, 1997;Munnik et al, 1995;Munnik et al, 1998b;Van Himbergen et al, 1999). As yet few PA-induced responses have been reported, but treatment of the green alga Chlamydomonas moewusii immediately resulted in de¯agellation (Munnik et al, 1995), while similar treatment of barley aleurone cells inhibited the secretion of a-amylase (Ritchie and Gilroy, 1998), and recently PA has been shown to induce stomatal closure (Jacob et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Hyperosmotic stress stimulates phospholipase D activity and elevates the levels of phosphatidic acid and diacylglycerol pyrophosphate

et al. 2000

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SummaryIn mammalian cells, phospholipase D (PLD) and its product phosphatidic acid (PA) are involved in a number of signalling cascades, including cell proliferation, membrane traf®cking and defence responses. In plant cells a signalling role for PLD and PA is also emerging. Plants have the extra ability to phosphorylate PA to produce diacylglycerol pyrophosphate (DGPP), a newly discovered phospholipid whose formation attenuates PA levels, but which could itself be a second messenger. Here we report that increases in PA and its conversion to DGPP are common stress responses to water de®cit. Increases occur within minutes of treatment and are dependent on the level of stress. Part of the PA produced is due to PLD activity as measured by the in vivo transphosphatidylation of 1-butanol, and part is due to diacylglycerol kinase activity as monitored via 32 P-PA formation in a differential labelling protocol. Increases in PA and DGPP are found not only in the green alga Chlamydomonas moewusii and cellsuspension cultures of tomato and alfalfa when subjected to hyperosmotic stress, but also in dehydrated leaves of the resurrection plant Craterostigma plantagineum. These results provide further evidence that PLD and PA play a role in plant signalling, and provide the ®rst demonstration that DGPP is formed during physiological conditions that evoke PA synthesis.

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Detailed analysis of the turnover of polyphosphoinositides and phosphatidic acid upon activation of phospholipases C and D in Chlamydomonas cells treated with non-permeabilizing concentrations of mastoparan

Cited by 110 publications

References 60 publications

The role of phospholipase D in plant stress responses

The role of phospholipase D in plant stress responses

Nod Factor and Elicitors Activate Different Phospholipid Signaling Pathways in Suspension-Cultured Alfalfa Cells

Hyperosmotic stress stimulates phospholipase D activity and elevates the levels of phosphatidic acid and diacylglycerol pyrophosphate

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