2004
DOI: 10.1111/j.1574-6968.2004.tb09673.x
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Detailed analysis of the insertion site of the mobile elements R997, pMERPH, R392, R705 and R391 inE. coliK12

Abstract: The IncJ group of mobile elements have not been extensively studied until recently, due to the inability to isolate extrachromosomal DNA from IncJ-strains. Sequence analysis of the prototype IncJ element, R391, revealed it to be a mosaic structure, integrated into the prfC gene in E. coli. Using inverse PCR (iPCR), we localised the other available IncJ elements (R392, R705, R997 and pMERPH) site of insertion to a 17-bp sequence, within the 5' end of prfC at 99.31 min on the E. coli chromosome, and confirmed th… Show more

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Cited by 21 publications
(5 citation statements)
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“…Conjugal transfer experiments confirm that Tn 6512 -related ICEs with the complete gene sets for conjugal transfer have the ability to transfer from the wild-type isolates of Proteus , 40 Vibrio , 41 Shewanella , 42 and Providencia (this study) to the recipient bacteria E. coli , and that a circular extrachromosomal Tn 6512 has the ability to transfer into the recipient bacteria Salmonella , 39 Enterobacter , 39 and Serratia , 39 indicating that this group of ICEs have a very broad host range.…”
Section: Discussionsupporting
confidence: 60%
See 1 more Smart Citation
“…Conjugal transfer experiments confirm that Tn 6512 -related ICEs with the complete gene sets for conjugal transfer have the ability to transfer from the wild-type isolates of Proteus , 40 Vibrio , 41 Shewanella , 42 and Providencia (this study) to the recipient bacteria E. coli , and that a circular extrachromosomal Tn 6512 has the ability to transfer into the recipient bacteria Salmonella , 39 Enterobacter , 39 and Serratia , 39 indicating that this group of ICEs have a very broad host range.…”
Section: Discussionsupporting
confidence: 60%
“…Integration of these ICEs needs the 17-bp relatively non-conserved attB (attachment site on the chromosome) target sites within the 5’ end of prfC , 32 and can occur with up to 23.5% sequence mismatch between attB and attP . 39 These attB sites together with Tn 6512 -related ICEs are widely distributed in Gram-negative bacteria as mentioned above. Taken together, Tn 7 -related unit transposons and Tn 6512 -related ICEs have a wide host range.…”
Section: Discussionmentioning
confidence: 95%
“…Coincidentally, R391 was also first discovered in a Providencia rettgeri clinical isolate (52) and was later classified as the SXT/R391 family (53)(54)(55). The ICE in strain RF14-2 integrated into the 5= end of the gene prfC, which was a typical characteristic of all members of the SXT/R391 family (55,56). This novel SXT/R391 ICE was designated ICEPreChnRF14-2 according to the nomenclature system (54).…”
mentioning
confidence: 99%
“…Type 1 elements have been found in a variety of Gammaproteobacteria species, including the Vibrio, Shewanella, Proteus, and Photobacterium (a full list can be found in Supplementary Table S1 ). They are distinguished by possessing a type 1 integrase, which allows for site-specific integration into the 5 end of the essential prfC gene [54]. Integration restores a functioning prf C gene and encodes a new hybrid PrfC protein [54].…”
Section: Introductionmentioning
confidence: 99%
“…They are distinguished by possessing a type 1 integrase, which allows for site-specific integration into the 5 end of the essential prfC gene [54]. Integration restores a functioning prf C gene and encodes a new hybrid PrfC protein [54]. Type 2,3,and 4 ICEs all insert at the 3 end of the multicopy tRNA-Ser gene and have been found solely in Vibrio species [3,55].…”
Section: Introductionmentioning
confidence: 99%