Detachable microfluidic device implemented with electrochemical aptasensor (DeMEA) for sequential analysis of cancerous exosomes

Kashefi‐Kheyrabadi, Leila; Kim, Junmoo; Chakravarty, Sudesna; Park, Sunyoung; Gwak, Hogyeong; Kim, Kyung Sik; Mohammadniaei, Mohsen; Lee, Min Ho; Hyun, Kyung A.; Jung, Hyo Il

doi:10.1016/j.bios.2020.112622

Cited by 77 publications

(45 citation statements)

References 29 publications

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“…Selective quantification of biomarkers in nanovesicles derived from cancerous tissues was performed using microfluidics-based on liquid biopsy screening tests which improved the accuracy of the results [ 121 ]. Highly sensitive cancerous exosomes were quantified using a detachable microfluidic device implemented with electrochemical aptasensor (DeMEA) which helped in the early detection of cancerous biomarkers thereby contributing to the early diagnosis (exosome-based cancer diagnosis) [ 122 ]. A microfluidic device called ExoChip was designed to isolate and quantify circulating exosomes obtained directly from the serum sample of blood, which are a promising diagnostic biomarkers source and therefore, can be utilized for molecular screening of cancers [ 72 ].…”

Section: Methods Of Quantificationmentioning

confidence: 99%

Elucidating Methods for Isolation and Quantification of Exosomes: A Review

et al. 2021

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Exosomes are the smallest extracellular vesicles present in most of the biological fluids. They are found to play an important role in cell signaling, immune response, tumor metastasis, etc. Studies have shown that these vesicles also have diagnostic and therapeutic roles for which their accurate detection and quantification is essential. Due to the complexity in size and structure of exosomes, even the gold standard methods face challenges. This comprehensive review discusses the various standard methods such as ultracentrifugation, ultrafiltration, size-exclusion chromatography, precipitation, immunoaffinity, and microfluidic technologies for the isolation of exosomes. The principle of isolation of each method is described, as well as their specific advantages and disadvantages. Quantification of exosomes by nanoparticle tracking analysis, flow cytometry, tunable resistive pulse sensing, electron microscopy, dynamic light scattering, and microfluidic devices are also described, along with the applications of exosomes in various biomedical domains.

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Section: Methods Of Quantificationmentioning

confidence: 99%

Elucidating Methods for Isolation and Quantification of Exosomes: A Review

et al. 2021

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“…ADD may use better targets for its aptasensors beyond RBD S1 and hACE2. ADD can be accomplished, as suggested by the evidence from creation of DeMEA 158 (but it uses high cost microfluidics 159 ).…”

Section: Discussionmentioning

confidence: 99%

Aptamers for Detection and Diagnostics (ADD) is a proposed mobile app acquiring optical data from conjugated quantum nanodots to identify molecules indicating presence of SARS-CoV-2 virus: Why public health and healthcare need smartphone sensors as a platform for early detection and prevention

Datta

2021

Preprint

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Proposed SARS-CoV-2 surveillance tool using a mobile app for non-invasive monitoring of humans and animals. <p>Engineering a biomedical device as a low-cost, non-invasive, detection, and diagnostic platform for surveillance of infections in humans, and animals. The system embraces the IoT <i>“digital by design”</i> metaphor by incorporating elements of connectivity, data sharing and (secure) information arbitrage. Using an array of aptamers to bind viral targets may help in detection, diagnostics, and potentially prevention in case of SARS-CoV-2. The ADD tool may become part of a broader platform approach.</p>

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“…Based on the precise recognition of biomolecules, [ 68,79 ] subcellular organisms, [ 80 ] and cells, [ 71,81 ] the nanomaterial‐based biochemical sensors can be integrated with other devices (e.g., microfluidics and wearable devices) toward health management and therapeutic controlling systems. [ 82 ]…”

Section: Biomedical Applicationsmentioning

confidence: 99%

Nanomaterial‐Based Electrochemical Sensors: Mechanism, Preparation, and Application in Biomedicine

Liu

Huang

Qian

2021

Advanced NanoBiomed Research

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Nanomaterials possess unique properties, including excellent electrochemical properties, high surface area, and tunable electric conductivity, making them attractive for sensing applications, especially for electrochemical sensing. Notably, the compatibility of nanomaterials with other techniques opens up opportunities for electrochemical sensors in various biomedical applications. Herein, the enhancing mechanisms and preparation protocols of electrochemical sensors, regarding the material choices, are introduced. The integration of nanomaterial‐based electrochemical sensors with other techniques toward precise health management and controlled drug release is further highlighted. It is concluded with perspectives on the future directions for this topic. Future research directions of nanomaterial‐based electrochemical sensors and the challenges faced by commercialized implementation of the sensors are presented, paving the way for the upcoming era of accurate biosensing toward both academic and industrial use.

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Detachable microfluidic device implemented with electrochemical aptasensor (DeMEA) for sequential analysis of cancerous exosomes

Cited by 77 publications

References 29 publications

Elucidating Methods for Isolation and Quantification of Exosomes: A Review

Elucidating Methods for Isolation and Quantification of Exosomes: A Review

Aptamers for Detection and Diagnostics (ADD) is a proposed mobile app acquiring optical data from conjugated quantum nanodots to identify molecules indicating presence of SARS-CoV-2 virus: Why public health and healthcare need smartphone sensors as a platform for early detection and prevention

Nanomaterial‐Based Electrochemical Sensors: Mechanism, Preparation, and Application in Biomedicine

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