DET1-mediated COP1 regulation avoids HY5 activity over second-site gene targets to tune plant photomorphogenesis

Cañibano, Esther; Bourbousse, Clara; García-León, Marta; Gómez, Borja Garnelo; Wolff, Léa; Garcia-Baudino, Camila; Lozano‐Durán, Rosa; Barneche, Frédy; Rubio, Vicente; Fonseca, Sandra

doi:10.1016/j.molp.2021.03.009

Cited by 28 publications

(26 citation statements)

References 127 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, the SSC protein was very stable and, moreover, also stabilized endogenous COP1. A role of DET1 in destabilizing COP1 was reported recently (Cañibano et al, 2021). Hence, SSC may be inactive due to a failure to interact with the CUL4‐RBX1‐DDB1‐DDA complex.…”

Section: Discussionmentioning

confidence: 57%

Functional comparison of the WD‐repeat domains of SPA1 and COP1 in suppression of photomorphogenesis

Kerner

Nagano

Lübbe

et al. 2021

Plant Cell & Environment

View full text Add to dashboard Cite

The Arabidopsis COP1/SPA complex acts as a cullin4‐based E3 ubiquitin ligase to suppress photomorphogenesis in darkness. It is a tetrameric complex of two COP1 and two SPA proteins. Both COP1 and SPA are essential for the activity of this complex, and they both contain a C‐terminal WD‐repeat domain responsible for substrate recruitment and binding of DDB1. Here, we used a WD domain swap‐approach to address the cooperativity of COP1 and SPA proteins. We found that expression of a chimeric COP1 carrying the WD‐repeat domain of SPA1 mostly complemented the cop1‐4‐mutant phenotype in darkness, indicating that the WD repeat of SPA1 can replace the WD repeat of COP1. In the light, SPA1‐WD partially substituted for COP1‐WD. In contrast, expression of a chimeric SPA1 protein carrying the WD repeat of COP1 did not rescue the spa‐mutant phenotype. Together, our findings demonstrate that a SPA1‐type WD repeat is essential for COP1/SPA activity, while a COP1‐type WD is in part dispensible. Moreover, a complex with four SPA1‐WDs is more active than a complex with only two SPA1‐WDs. A homology model of SPA1‐WD based on the crystal structure of COP1‐WD uncovered two insertions and several amino acid substitutions at the predicted substrate‐binding pocket of SPA1‐WD.

show abstract

Section: Discussionmentioning

confidence: 57%

Functional comparison of the WD‐repeat domains of SPA1 and COP1 in suppression of photomorphogenesis

Kerner

Nagano

Lübbe

et al. 2021

Plant Cell & Environment

View full text Add to dashboard Cite

show abstract

“…DE-ETIOLATED1 (DET1) is a major repressor of photomorphogenesis that is associated with CULLIN4 (CUL4)-based E3 ubiquitin ligases for the degradation of several transcription factors [ 43 ]. Indeed, a novel regulatory network involving DET1–COP1–HY5 has been described in A. thaliana photomorphogenesis [ 44 ]. Whether a conserved regulatory module downstream of DET1 and involving HY5 is required for wound-induced de novo shoot formation in tomato hypocotyl explants needs to be addressed.…”

Section: Discussionmentioning

confidence: 99%

Tissue-Specific Metabolic Reprogramming during Wound-Induced Organ Formation in Tomato Hypocotyl Explants

Larriba

Sánchez-García

Martínez‐Andújar

et al. 2021

IJMS

View full text Add to dashboard Cite

Plants have remarkable regenerative capacity, which allows them to survive tissue damage after exposure to biotic and abiotic stresses. Some of the key transcription factors and hormone crosstalk mechanisms involved in wound-induced organ regeneration have been extensively studied in the model plant Arabidopsis thaliana. However, little is known about the role of metabolism in wound-induced organ formation. Here, we performed detailed transcriptome analysis and used a targeted metabolomics approach to study de novo organ formation in tomato hypocotyl explants and found tissue-specific metabolic differences and divergent developmental pathways. Our results indicate that successful regeneration in the apical region of the hypocotyl depends on a specific metabolic switch involving the upregulation of photorespiratory pathway components and the differential regulation of photosynthesis-related gene expression and gluconeogenesis pathway activation. These findings provide a useful resource for further investigation of the molecular mechanisms involved in wound-induced organ formation in crop species such as tomato.

show abstract

“…HY5 is a transcription factor that promotes photomorphogenesis in light, acting downstream of the light receptor network and directly activating the transcription of light-induced genes. Among these are genes encoding transcription factors, signaling proteins, and flavonoid biosynthetic enzymes ( Gangappa and Botto, 2016 ; Cañibano et al, 2021 ). PhyB-dependent induction of HY5 is well known ( Guo et al, 2021 ).…”

Section: Hy5-based Modulesmentioning

confidence: 99%

“…Because DET1 and COP1 are repressors of photomorphogenesis and repressors of multiple light responses, destruction of any of these proteins results in the induction of de-etiolation in darkness and hyper-photomorphogenic phenotype in the light. DET1 is associated with COP1 in vivo ( Cañibano et al, 2021 ), and DET1/COP1 and HY5 are now considered as two main distinct signaling components (albeit with some overlap of function) in the regulation of thermosensory elongation ( Xu et al, 2020 ). The DET1/COP1 association promotes PIF4 expression and stabilizes PIF4 protein ( Gangappa and Kumar, 2017 , 2018 ).…”

Section: Fhy3/far1 and Det1 In The Process Of Interaction With Aba Signalingmentioning

confidence: 99%

Basic Protein Modules Combining Abscisic Acid and Light Signaling in Arabidopsis

Bulgakov

Koren

2022

Front. Plant Sci.

View full text Add to dashboard Cite

It is generally accepted that plants use the complex signaling system regulated by light and abscisic acid (ABA) signaling components to optimize growth and development in different situations. The role of ABA–light interactions is evident in the coupling of stress defense reactions with seed germination and root development, maintaining of stem cell identity and stem cell specification, stem elongation and leaf development, flowering and fruit formation, senescence, and shade avoidance. All these processes are regulated jointly by the ABA–light signaling system. Although a lot of work has been devoted to ABA–light signal interactions, there is still no systematic description of central signaling components and protein modules, which jointly regulate plant development. New data have emerged to promote understanding of how ABA and light signals are integrated at the molecular level, representing an extensively growing area of research. This work is intended to fill existing gaps by using literature data combined with bioinformatics analysis.

show abstract

DET1-mediated COP1 regulation avoids HY5 activity over second-site gene targets to tune plant photomorphogenesis

Cited by 28 publications

References 127 publications

Functional comparison of the WD‐repeat domains of SPA1 and COP1 in suppression of photomorphogenesis

Functional comparison of the WD‐repeat domains of SPA1 and COP1 in suppression of photomorphogenesis

Tissue-Specific Metabolic Reprogramming during Wound-Induced Organ Formation in Tomato Hypocotyl Explants

Basic Protein Modules Combining Abscisic Acid and Light Signaling in Arabidopsis

Contact Info

Product

Resources

About