Desulfurococcaceae, the Second Family of the Extremely Thermophilic, Anaerobic, Sulfur-Respiring Thermoproteales

Zillig, Wolfram; Stetter, Karl O.; Prangishvilli, David; Schäfer, Wolfram; Wunderl, Simon; Janekovic, D.; Holz, Ingelore; Palm, Peter

doi:10.1016/s0721-9571(82)80044-6

Cited by 67 publications

(100 citation statements)

References 14 publications

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“…The latter organisms yield, upon fractionation, undissociated 70-S . ribosomes [24,25] whose poly(Phe)-synthesizing capacity is unimpaired by monovalent cations 16, 71 and which exhibit no absolute polyamine dependence, although low spermine levels (0.2 -1 .o mM) do enhance their synthetic activity [26].…”

Section: Sulfofobusmentioning

confidence: 99%

Differential features of ribosomes and of poly(U)‐programmed cell‐free systems derived from sulphur‐dependent archaebacterial species

Londei

Altamura

Cammarano

et al. 1986

European Journal of Biochemistry

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The properties of poly(U)-directed cell-free systems developed from the sulphur-dependent, thermophilic archaebacteria Desulfurococcus mobilis, Thermoproteus tenax, Sulfolobus solfataricus, Thermococcus celer and Thermoplasma acidophilum have been compared. All systems are truly thermophlic in requiring incubation at temperatures close to the physiological optimum for cell growth. Under optimized conditions the error frequency in tRNA selection is less than 0.4% at S O T , and synthetic efficiencies (Phe residues polymerized per ribosome in 40 min) span from 4 for Tp. tenax, to 10 for Tc. celer, to 20-25 for D. mobilis and T. acidophilum and to 40 for S . solfataricus. According to requirements for polypeptide synthesis and to degree of stability of the ribosomal subunits' association, sulphur-dependent thermophiles cluster into two groups. Group I organisms (D. mobilis, Tp. tenax, S. solfataricus) harbour 7 0 4 monomers composed of weakly associated subunits, whose poly(Phe)-synthesizing capacity is totally dependent on added spermine while being drastically inhibited by monovalent cations. Group I1 organisms (Tc. celer and T. acidophilum) contain 70-S particles composed of tightly bonded subunits, whose synthetic capacity is independent of spermine while being totally dependent on monovalent cations. Spermine promotes poly(Phe) synthesis on ribosomes of group I organisms by converting the peptidyltransferase center into an active conformation, while monovalent cations are inhibitory by preventing the interaction between the free ribosomal subunits. The closeness between Tc. celer and T. acidophilum ribosomes provides new insight on the phylogenetic placement of Thermococcaceae.The identification of archaebacteria as a third primary kingdom [ l ] has focused considerable attention on the translation apparatus of these unusual prokaryotes. Archaebacterial protein synthesis is known to be initiated by a non-formylated methionine [2] and most archaebacterial tRNAs have been found to harbour uniquely modified bases [3]. Archaebacterial ribosomes possess distinctive three-dimensional features [4, 51 and display unique mosaics of binding sites for both 70-S and 80-S ribosome-targeted inhibitors of protein synthesis [6 -81. Archaebacterial elongation factors are also unique in their response to factor-targeted inhibitors of eubacterial and/or eukaryotic protein synthesis [9 -111.In one report, elongation factors of certain archaebacteria (Methanococcus vannielii and Thermoplusma acidophilum) were shown to be functionally restricted to ribosomes of their own lineage [12]. Aside from that, little is yet known about the intralineage constancy of the archaebacterial translational components and the relatedness of the latter to components of eubacteria and eukaryotes. Efficient protein-synthesizing cell-free systems, covering a wide range of taxonomically diverse archaebacteria, are prerequisites to investigating these points. Abbreviations. AcPhe, N-acetylphenylalanine; poly(Phe), poly-(phenylalanine); DSM, Deursche Su...

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Section: Sulfofobusmentioning

confidence: 99%

Differential features of ribosomes and of poly(U)‐programmed cell‐free systems derived from sulphur‐dependent archaebacterial species

Londei

Altamura

Cammarano

et al. 1986

European Journal of Biochemistry

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“…The description is based mainly on that of Zillig et al (1982). Cells are cocci, obligately anaerobic and hyperthermophilic.…”

Section: Emended Description Of Desulfurococcus Mucosus Zillig and Stmentioning

confidence: 99%

“…

Reclassification ofRepresentatives of the genus Desulfurococcus were among the first hyperthermophilic archaea to be isolated and described (Zillig et al, 1982;Zillig & Stetter, 1983). All Desulfurococcus species are obligate anaerobes with coccoid cells; they belong to the family Desulfurococcaceae and the order Desulfurococcales in the archaeal phylum Crenarchaeota.

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mentioning

confidence: 99%

“…All Desulfurococcus species are obligate anaerobes with coccoid cells; they belong to the family Desulfurococcaceae and the order Desulfurococcales in the archaeal phylum Crenarchaeota. The first two species described, Desulfurococcus mucosus and Desulfurococcus mobilis, were isolated from Icelandic terrestrial hot springs; they were characterized as organotrophs, capable of utilizing a wide range of proteinaceous substrates by fermentation, facultatively coupled with the reduction of elemental sulfur to hydrogen sulfide (Zillig et al, 1982). Other members of the genus were IP: 54.186.52.231…”

mentioning

confidence: 99%

“…Differentiation between D. mucosus, D. mobilis (Zillig et al, 1982) and D. amylolyticus (Bonch-Osmolovskaya et al, 1988) was based on phenotypic distinctions. In addition, differences in 16S rRNA genes from those of D. mobilis (Kjems et al, 1987) and D. amylolyticus (Tourova et al, 2000) were then used for the proposal of D. fermentans (Perevalova et al, 2005) and D. kamchatkensis (Kublanov et al, 2009).…”

mentioning

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Reclassification of Desulfurococcus mobilis as a synonym of Desulfurococcus mucosus, Desulfurococcus fermentans and Desulfurococcus kamchatkensis as synonyms of Desulfurococcus amylolyticus, and emendation of the D. mucosus and D. amylolyticus species descriptions

Perevalova¹,

Kublanov²,

Bidzhieva³

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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Reclassification ofRepresentatives of the genus Desulfurococcus were among the first hyperthermophilic archaea to be isolated and described (Zillig et al., 1982;Zillig & Stetter, 1983). All Desulfurococcus species are obligate anaerobes with coccoid cells; they belong to the family Desulfurococcaceae and the order Desulfurococcales in the archaeal phylum Crenarchaeota. The first two species described, Desulfurococcus mucosus and Desulfurococcus mobilis, were isolated from Icelandic terrestrial hot springs; they were characterized as organotrophs, capable of utilizing a wide range of proteinaceous substrates by fermentation, facultatively coupled with the reduction of elemental sulfur to hydrogen sulfide (Zillig et al., 1982). Other members of the genus were Abbreviations: ANI, average nucleotide identity; DDH, DNA-DNA hybridization.

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Extremely Thermophilic Archaebacteria: Biological and Engineering Considerations

Kelly

Deming

1988

Biotechnology Progress

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~ Interdisciplinary interactions between molecular biologists, microbial ecologists, and biochemical engineers portend weU for the biotechnological exploitation of novel, extremely thermophdic methanogens and sulfur-metabolizing archaebacteriu.[ntroduction The field of biotechnology presently relies heavily on the use of genetic engineering to make the production of a variety of biological products feasme in both a technical ind economic sense. However, there is growing interest in determining if "wild-type'' microorganisms that function under extreme conditions (i.e., high temperature, high pressure, low pH, etc.) or their products can be put directly to effective biotechnological use. The exploitation of these novel microorganisms need not preclude the use of recombinant DNA technology; on the contrary, the more we learn about the "natural" functions of bacteria from extreme environments, the greater the possibilities for genetic engineering. Among the extreme environments in which microorganisms have been found to prosper, few have generated as much scientific interest in recent years as those characterized by high temperatures [ I ] . Following reports of evidence that bacterial growth and activity may occur under deep-sea pressures at temperatures to at least 250°C [ 2 4 ] (well in excess of conventional limits) efforts by several groups of researchers [S7] to acquire more samples from submarine hydrothermal vents, hot springs, and other areas of geothermal activity in search of novel extremely thermophilic microorganisms have intensified. Though these efforts have not yet yielded confirmation of the original report [3], not quelled the controversy (pro [8] and con [9-111) that it engendered, there has been notable progress toward the isolation of new thermophiles that #Correspondence should be address to Robert M . Kelly Yody W. Deming is presently at the School of Oceanography llniversity of Washington, WB-10, Seattle, WA 98195. grow at higher and higher temperatures. In particular, Stetter and colleagues [5, 6, 12, 131 have reported and

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Desulfurococcaceae, the Second Family of the Extremely Thermophilic, Anaerobic, Sulfur-Respiring Thermoproteales

Cited by 67 publications

References 14 publications

Differential features of ribosomes and of poly(U)‐programmed cell‐free systems derived from sulphur‐dependent archaebacterial species

Differential features of ribosomes and of poly(U)‐programmed cell‐free systems derived from sulphur‐dependent archaebacterial species

Reclassification of Desulfurococcus mobilis as a synonym of Desulfurococcus mucosus, Desulfurococcus fermentans and Desulfurococcus kamchatkensis as synonyms of Desulfurococcus amylolyticus, and emendation of the D. mucosus and D. amylolyticus species descriptions

Extremely Thermophilic Archaebacteria: Biological and Engineering Considerations

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