Desulfurispirillum indicum sp. nov., a selenate- and selenite-respiring bacterium isolated from an estuarine canal

Rauschenbach, I. Yu.; Narasingarao, Priya; Häggblom, Max M.

doi:10.1099/ijs.0.022392-0

Cited by 47 publications

(24 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2). In contrast, DNA analysis detected, in addition, bacteria of the orders Methanosarcinales , Actinomyindales and Bifidobacteriales .The few bacteria that have been classified within the order Chrysiogenales are environmental organisms able to respire arsenate or selenium [23], [24]. As far as we are aware however, no association of bacteria from this order with CF or other infections has been made previously.…”

Section: Resultsmentioning

confidence: 72%

“…The absence of previous reports of Chrysiogenales in CF may reflect difficulties in their cultivation. The only member of this order that has been characterised thus far is Chrysiogenes arsenatis , an obligately anaerobic organism isolated from estuarine environments that respires arsenate or selenium [23], [24]. Selected typed strains of Chrysiogenes arsenatis are at various stages of genome sequencing [34] and a draft genome sequence is available.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Microbiota and Metabolite Profiling Reveal Specific Alterations in Bacterial Community Structure and Environment in the Cystic Fibrosis Airway during Exacerbation

Twomey

Alston

et al. 2013

PLoS ONE

View full text Add to dashboard Cite

Chronic polymicrobial infections of the lung are the foremost cause of morbidity and mortality in cystic fibrosis (CF) patients. The composition of the microbial flora of the airway alters considerably during infection, particularly during patient exacerbation. An understanding of which organisms are growing, their environment and their behaviour in the airway is of importance for designing antibiotic treatment regimes and for patient prognosis. To this end, we have analysed sputum samples taken from separate cohorts of CF and non-CF subjects for metabolites and in parallel, and we have examined both isolated DNA and RNA for the presence of 16S rRNA genes and transcripts by high-throughput sequencing of amplicon or cDNA libraries. This analysis revealed that although the population size of all dominant orders of bacteria as measured by DNA- and RNA- based methods are similar, greater discrepancies are seen with less prevalent organisms, some of which we associated with CF for the first time. Additionally, we identified a strong relationship between the abundance of specific anaerobes and fluctuations in several metabolites including lactate and putrescine during patient exacerbation. This study has hence identified organisms whose occurrence within the CF microbiome has been hitherto unreported and has revealed potential metabolic biomarkers for exacerbation.

show abstract

Section: Resultsmentioning

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Microbiota and Metabolite Profiling Reveal Specific Alterations in Bacterial Community Structure and Environment in the Cystic Fibrosis Airway during Exacerbation

Twomey

Alston

et al. 2013

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…Sedimenticola selenatireducens was isolated using 4-hydroxybenzyoate and SeO 4 2Ϫ as the sole electron donor and acceptor, respectively (48). Although Desulfurispirillum indicum was isolated using pyruvate and SeO 4 2Ϫ as the sole electron donor and acceptor, respectively, the strain was able to couple the oxidation of other short-chain organic acids, such as lactate and acetate, to SeO 4 2Ϫ reduction (49). The D. indicum strain demonstrated metabolic flexibility to respire SeO 4 2Ϫ to SeO 3 2Ϫ and then to elemental selenium under anaerobic conditions.…”

Section: Selenate-respiring Bacteriamentioning

confidence: 99%

Ecology and Biotechnology of Selenium-Respiring Bacteria

Nancharaiah

2015

Microbiol Mol Biol Rev

348

232

View full text Add to dashboard Cite

SUMMARY In nature, selenium is actively cycled between oxic and anoxic habitats, and this cycle plays an important role in carbon and nitrogen mineralization through bacterial anaerobic respiration. Selenium-respiring bacteria (SeRB) are found in geographically diverse, pristine or contaminated environments and play a pivotal role in the selenium cycle. Unlike its structural analogues oxygen and sulfur, the chalcogen selenium and its microbial cycling have received much less attention by the scientific community. This review focuses on microorganisms that use selenate and selenite as terminal electron acceptors, in parallel to the well-studied sulfate-reducing bacteria. It overviews the significant advancements made in recent years on the role of SeRB in the biological selenium cycle and their ecological role, phylogenetic characterization, and metabolism, as well as selenium biomineralization mechanisms and environmental biotechnological applications.

show abstract

“…Other results indicate that similarity to other proteins is limited only to a region covering amino acids 100-200 from the start methionine. One of these was identified in the genome of Desulfurispirillum indicum strain S5 (Selin_0231) (22), a known selenate respiring bacterium. Other hits reveal similarity to S-layer proteins, such as SapA from Campylobacter fetus.…”

Section: Secreted Protein Profile During Reduction Of Selenate and Sementioning

confidence: 99%

A bacterial process for selenium nanosphere assembly

Debieux

Dridge

Mueller

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

172

143

View full text Add to dashboard Cite

During selenate respiration by Thauera selenatis, the reduction of selenate results in the formation of intracellular selenium (Se) deposits that are ultimately secreted as Se nanospheres of approximately 150 nm in diameter. We report that the Se nanospheres are associated with a protein of approximately 95 kDa. Subsequent experiments to investigate the expression and secretion profile of this protein have demonstrated that it is up-regulated and secreted in response to increasing selenite concentrations. The protein was purified from Se nanospheres, and peptide fragments from a tryptic digest were used to identify the gene in the draft T. selenatis genome. A matched open reading frame was located, encoding a protein with a calculated mass of 94.5 kDa. N-terminal sequence analysis of the mature protein revealed no cleavable signal peptide, suggesting that the protein is exported directly from the cytoplasm. The protein has been called Se factor A (SefA), and homologues of known function have not been reported previously. The sefA gene was cloned and expressed in Escherichia coli, and the recombinant His-tagged SefA purified. In vivo experiments demonstrate that SefA forms larger (approximately 300 nm) Se nanospheres in E. coli when treated with selenite, and these are retained within the cell. In vitro assays demonstrate that the formation of Se nanospheres upon the reduction of selenite by glutathione are stabilized by the presence of SefA. The role of SefA in selenium nanosphere assembly has potential for exploitation in bionanomaterial fabrication.nanoparticles | biomineralization | anaerobic respiration

show abstract

Desulfurispirillum indicum sp. nov., a selenate- and selenite-respiring bacterium isolated from an estuarine canal

Cited by 47 publications

References 26 publications

Microbiota and Metabolite Profiling Reveal Specific Alterations in Bacterial Community Structure and Environment in the Cystic Fibrosis Airway during Exacerbation

Microbiota and Metabolite Profiling Reveal Specific Alterations in Bacterial Community Structure and Environment in the Cystic Fibrosis Airway during Exacerbation

Ecology and Biotechnology of Selenium-Respiring Bacteria

A bacterial process for selenium nanosphere assembly

Contact Info

Product

Resources

About