Desulfation of sulfated galactans with chlorotrimethylsilane. Characterization of β-carrageenan by 1H NMR spectroscopy

Selective sulfation of carrageenans and the influence of sulfate regiochemistry on anticoagulant properties

“…Sample 6 showed a decrease in DS (1.1-0.1) together with a reduction in the molecular weight (Table 3). The 13 C and 1 H NMR spectra were similar to those reported for beta-carrageenan (G-DA) (Kolender & Matulewicz, 2004) indicating that this diad is the main constituent of Sample 6 ( Table 3).…”

“…While several approaches have been reported for the desulfation of carbohydrates (Kolender & Matulewicz, 2004;Miller & Blunt, 1998;Takano, Kanda, Hayashi, Yoshida, & Hara, 1995), in this work, we selected the Nagasawa, Inoue, and Kamata (1977) method. Sample 6 showed a decrease in DS (1.1-0.1) together with a reduction in the molecular weight (Table 3).…”

Structural analysis and cytokine-induced activity of gelling sulfated polysaccharide from the cystocarpic plants of Ahnfeltiopsis flabelliformis

“…1). Chemical shifts of signals in the 1 H and 13 C NMR spectra of the gelling polysaccharide were compared to the signals of known carrageenan structures (Kolender & Matulewicz, 2004;Miller & Blunt, 2000; Van de Velde, Knutsen, Usov, Rollema, & Cerezo, 2002). Six signals with different intensities at 92.9, 95.9, 96.2, 102.9, 103.1 and 103.2 ppm were observed in the anomeric area of the 13 C NMR spectrum of the polysaccharide (Fig.…”

Structural characterisation and bioactivities of hybrid carrageenan-like sulphated galactan from red alga Furcellaria lumbricalis

“…Desulphation was operated as described (Kolender & Matulewicz, 2004), but with some modification. In brief, FB1 (20 mg) was passed through an Amberlite IR 120 column (20 ml).…”