Destructive and reparative processes in the liver in acute experimental peritonitis

Glumov, V. Ya.; Ivanova, G. S.; Баженов, Е. Л.

doi:10.1007/bf00840111

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“…The character of the liver lesions was similar to that previously described in earlier studies of LPS-mediated liver cell damage [18,24] and in an acute experimental peritonitis model [4]. At the early time points (days 3 and 7) the severity and character of liver damage was indistinguishable in CD14-/-and CD14+/+ mice; histological analysis of the liver showed a similar degree of apoptosis and polymorphonuclear and mononuclear infiltration as well as necrosis in both populations of mice.…”

Section: Discussionsupporting

confidence: 69%

Reduced bacterial dissemination and liver injury in CD14-deficient mice following a chronic abscess-forming peritonitis induced by Bacteroides fragilis

Woltmann

Gangloff

Bruch

et al. 1999

Medical Microbiology and Immunology

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The CD14 myelomonocytic differentiation antigen plays a major role in acute Gram-negative infections with Escherichia coli; however, its role in chronic infections has not yet been analyzed. To address this question, we studied the role of CD14 in a chronic abscess-forming peritonitis, induced by Bacteroides fragilis. B. fragilis (3x10(8) CFU/ml) were resuspended in a liquid nutrient agar and injected into the peritoneal cavity of CD14-deficient (CD 14-/-) and normal C57BL/6J (CD 14+/+) mice, respectively. After 3 days there was a severe phlegmonous intra-abdominal inflammation in both groups. After 7 days an abscess-forming peritonitis developed and by 14 days the infectious foci were compartimentalized. These observations were indistinguishable between CD14-/- and CD14+/+ mice. Although no differences were seen in abscess formation, CD14-/- mice were able to clear B. fragilis more efficiently from the blood than CD14+/+ mice. After 3, 7, and 14 days blood cultures were B. fragilis positive in 11% (1/9), 20% (2/10), and 0% (0/9) in CD14-/-compared with 90% (9/10), 78% (7/9), and 20% (2/10) in CD14+/+ mice, respectively (P<0.05). Furthermore, although the infection resulted in hepatocellular necrosis and severe hepatitis in both groups, at day 14 the liver cell damage was more severe in CD14+/+ than in CD14-/- mice (P<0.05). These results show that the chronic abscess formation induced by B. fragilis capsular polysaccharides is CD14 independent; however, bacterial clearance and/or dissemination and liver cell damage are at least partially influenced by CD14-dependent mechanisms.

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Section: Discussionsupporting

confidence: 69%