Destruction of Bacteria in the Digestive Tract of the Maggot of<i>Lucilia sericata</i>(Diptera: Calliphoridae)

Mumcuoglu, Kosta Y.; Miller, Jacqueline M.; Mumcuoglu, Michael; Friger, Michael; Tarshis, Mark

doi:10.1603/0022-2585-38.2.161

Cited by 196 publications

(158 citation statements)

References 16 publications

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“…fibroblasts on extracellular matrix, stimulate proliferation and migration of fibroblasts, and therefore enhance tissue formation and accelerate healing [10]. In another study, the mechanical action of maggots has been suggested in aiding tissue débridement [17]. One of the most intriguing hypotheses regarding the effectiveness of maggots in infected wounds is the idea that Lucilia sericata ES have antibacterial properties.…”

Section: Discussionmentioning

confidence: 99%

The Influence of Maggot Excretions on PAO1 Biofilm Formation on Different Biomaterials

Cazander

Veen

Bouwman

et al. 2009

Clinical Orthopaedics &Amp; Related Research

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Biofilm formation in wounds and on biomaterials is increasingly recognized as a problem. It therefore is important to focus on new strategies for eradicating severe biofilm-associated infections. The beneficial effects of maggots (Lucilia sericata) in wounds have been known for centuries. We hypothesized sterile maggot excretions and secretions (ES) could prevent, inhibit, and break down biofilms of Pseudomonas aeruginosa (PAO1) on different biomaterials. Therefore, we investigated biofilm formation on polyethylene, titanium, and stainless steel. Furthermore, we compared the biofilm reduction capacity of Instar-1 and Instar-3 maggot ES and tested the temperature tolerance of ES. After biofilms formed in M63 nutrient medium on comb-forming models of the biomaterials, ES solutions in phosphate-buffered saline or M63 were added in different concentrations. PAO1 biofilms adhered tightly to polyethylene and titanium but weakly to stainless steel. Maggot ES prevent and inhibit PAO1 biofilm formation and even break down existing biofilms. ES still had considerable biofilm reduction properties after storage at room temperature for 1 month. ES from Instar-3 maggots were more effective than ES from Instar-1 maggots. These results may be relevant to patient care as biofilms complicate the treatment of infections associated with orthopaedic implants.

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Section: Discussionmentioning

confidence: 99%

The Influence of Maggot Excretions on PAO1 Biofilm Formation on Different Biomaterials

Cazander

Veen

Bouwman

et al. 2009

Clinical Orthopaedics &Amp; Related Research

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“…Existen grandes avances en el estudio de los mecanismos de acción de la terapia con larvas sobre algunos patógenos que infectan las lesiones de piel por úlceras varicosas, podopatía diabética u osteomielitis, como S. aureus, P. aeruginosa, E. coli y Streptococcus spp. (16,17), y en la cual están involucradas larvas necrobiontófagas, como las de L. sericata.…”

Section: Discussionunclassified

Evaluación preliminar en un modelo animal de la terapia con larvas de Lucilia sericata para el tratamiento de la leishmaniasis cutánea

2008

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Introducción. La terapia con larvas ha sido ampliamente utilizada para el tratamiento de lesiones ulcerativas de la piel; existen registros de enfermedades, como podopatía diabética, osteomielitis y úlceras varicosas, en las cuales el uso de la terapia con larvas ha promovido la cicatrización de la lesión en presencia de patógenos bacterianos resistentes a los antibióticos convencionales. Objetivo. Realizar una prueba piloto de terapia con larvas de Lucilia sericata sobre lesiones cutáneas producidas por Leishmania amazonensis. Materiales y métodos. En el presente trabajo se empleó un diseño experimental en animales (Mesocricetus aureatus, tres réplicas) con la finalidad de analizar las variaciones del tamaño de la lesión por leishmaniasis antes y después de la terapia con larvas de L. sericata, utilizando criterios para la evaluación de la terapia tales como tamaño de la lesión, tiempo de aplicación y presencia de edema y secreción. Resultados. Indican una cicatrización efectiva y curación de las lesiones localizadas después de la terapia con larvas de L. sericata (80% a 100% de reducción del área de la lesión en 12 horas). Discusión. Los resultados preliminares indican que las larvas de moscas L. sericata son de uso potencial como terapia natural alternativa médica y veterinaria para la leishmaniasis cutánea. Preliminary evaluation of maggot (Diptera: Calliphoridae) therapy as a potential treatment for leishmaniasis ulcersIntroduction. Maggot debridement therapy has been widely used for treating a variety of scarredover soft-tissue wounds. Published accounts record several illnesses in which treatment with larval therapy has promoted injury healing in conjunction with infection by bacterial pathogens resistant to conventional antibiotics.Objective. An initial test of the maggot therapy was developed for cutaneous injuries produced by Leishmania amazonensis. Materials and methods. An experimental design based on an animal model with three replicates in Mesocricetus aureatus (Rodentia: Muridae) was used to evaluate size variation lesion before and after after larval therapy with Lucilia sericata maggots. The criteria used for therapy evaluation were lesion size, maggot application time, and presence or absence of edema and secretions. Results. Effective scarring and wound healing was observed after therapy with L. sericata larvae, i.e. 80% to 100% lesion area reduction after 12 hours. Conclusion. The preliminary results suggest that fly maggots of L. sericata have a potential use as natural medical and veterinary alternative therapy for the cutaneous leishmaniasis.

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“…Larva treatment was continued, if the wound did not close during follow-up. [10,11,12] There were four to eight applications for 29 wounds (78.9%) and 10 to 14 for seven wounds (21.1%).…”

Section: Methodsmentioning

confidence: 99%

“…[5,6] Larval excretions have been found to contain at least two antibacterial substances with one similar to a hydrophobic peptide of 3-10 kDa and the other a hydrophilic substance of 1 kDa. In 2007, Huberman et al [7] reported that [7][8][9][10][11] The larvae remove the dead tissue over the wound with the enzymes they secrete, disinfect the lesion by ingesting, killing, and halting the proliferation of the microorganisms, and stimulate the tissue for granulation development. [5,6] In the present study, we aimed to use larvae of the L. sericata fly species to treat pressure ulcers which were resistant to hyperbaric oxygen treatment, VAC, surgical debridement, or classic treatment methods used for a long period.…”

mentioning

confidence: 99%

Treatment of pressure ulcers with larvae of Lucilia sericata

Polat

Kutlubay

Sirekbasan

et al. 2017

Turk J Phys Med Rehab

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Objectives: This study aims to investigate the use of the Lucilia sericata larvae in patients with decubitus ulcers resistant to hyperbaric oxygen treatment, vacuum-assisted closure, surgical debridement, and other conventional therapies. Patients and methods:A total of 36 patients (21 females, 15 males; mean age 63.7 years; range 16 to 90 years) who were admitted to our hospital for decubitus ulcers between February 2011 and July 2016 were included in our study. All patients had one or more lesions in the sacral region, trochanteric area, dorsal region, or on the heel of the foot. Nine patients had spinal cord injuries, six of them were injured during a car accident, and three of them had spinal cord injuries due to gunshot. One patient had concomitant lung cancer, one had heart failure, and two patients had a diagnosis of multiple sclerosis. Thirty three of 36 patients were admitted to the wound therapy unit within the department of emergency medicine, and three were admitted to the physical therapy and rehabilitation clinic. For each patient, Lucilia sericata maggots were applied on the lesions for 72 hours and, then, washed away. This procedure was repeated two times a week. Twenty nine patients (78.9%) had four to six sessions and seven patients (21.1%) had eight to 12 sessions.Results: Twenty nine lesions (78.9%) were Grade 2 and 3 and were completely healed after four to eight treatment sessions, while seven lesions (21.1%) were completely cured at the end of 10 to 14 sessions. There was complete clearance of necrotic debris at the end of two sessions within the first week for 15 lesions, whereas 10 lesions (27.8%) were cured after four sessions within two weeks, seven lesions (19.4%) within five sessions after three weeks, and four lesions (11.1%) were treated at the end of seven sessions for four weeks. All the necrotic crusts over the surface of the ulcers were cleaned and bad odor of the lesions disappeared. Conclusion:Lucilia sericata larvae debridement is a rapid and effective treatment option for the management of chronic decubitus ulcers which are resistant to conventional therapies and other treatment modalities such as hyperbaric oxygen, vacuum-assisted closure, and surgical debridement.

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Destruction of Bacteria in the Digestive Tract of the Maggot ofLucilia sericata(Diptera: Calliphoridae)

Cited by 196 publications

References 16 publications

The Influence of Maggot Excretions on PAO1 Biofilm Formation on Different Biomaterials

The Influence of Maggot Excretions on PAO1 Biofilm Formation on Different Biomaterials

Evaluación preliminar en un modelo animal de la terapia con larvas de Lucilia sericata para el tratamiento de la leishmaniasis cutánea

Treatment of pressure ulcers with larvae of Lucilia sericata

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