Destiny of <i>Dendrobium officinale</i> Polysaccharide after Oral Administration: Indigestible and Nonabsorbing, Ends in Modulating Gut Microbiota

Li, Lifeng; Yao, Hong; Li, Xiaojun; Zhang, Quanwei; Wu, Xiyang; Wong, Tin-Long; Hai, Zheng; Fung, Hau-Yee; Yang, Baocheng; Ma, Dik‐Lung; Leung, Chung‐Hang; Zhang, Ge; Bian, Zhaoxiang; Lu, Aiping; Han, Quan‐Bin

doi:10.1021/acs.jafc.9b01489

Cited by 114 publications

(66 citation statements)

References 32 publications

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“…Pueraria lobata comes from the dried roots of leguminous plants and has been shown to have medicinal value. Pueraria polysaccharides are extracted from P. Lobata and can be consumed by intestinal microorganisms to benefit metabolism, ultimately affecting the body's physiological function (19,20). After it is fermented and metabolized in the intestinal flora, this TCM can effectively improve drug properties and reduce drug toxicity (21).…”

Section: Pueraria Lobatamentioning

confidence: 99%

Traditional Chinese medicine, liver fibrosis, intestinal flora: is there any connection?—a narrative review

Liu¹,

Qi²,

Sun³

2021

Ann Palliat Med

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This paper aims to analyze how intestinal flora regulates liver fibrosis pathogenesis and to evaluate the regulatory effect of traditional Chinese medicine on the intestinal flora, providing new insights into liver fibrosis treatment. Destruction of the intestinal microbiome can lead to liver fibrosis development, accelerating the intestinal microbiome's disruption. Traditional Chinese Medicine can effectively regulate the intestinal flora, helping prevent and treat liver fibrosis. This review discusses the mechanisms behind intestinal flora changes in liver fibrosis and how traditional Chinese medicine can regulate these changes.We searched PubMed, the Wanfang database, and CNKI for "liver fibrosis", "intestinal microflora", and "intestinal microbiota" and reviewed the retrieved literature. We detail the prevention and treatment options for liver fibrosis though the use of traditional Chinese medicine in regulating intestinal flora. We also highlight the influence of the intestinal flora on liver fibrosis and present the research regarding the prevention and treatment of liver fibrosis using traditional Chinese medicine. We also describe the effects of traditional Chinese medicine on the intestinal flora. Traditional Chinese medicine can effectively regulate the intestinal flora to prevent and treat liver fibrosis through the liver-intestine axis.

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Section: Pueraria Lobatamentioning

confidence: 99%

Traditional Chinese medicine, liver fibrosis, intestinal flora: is there any connection?—a narrative review

Liu¹,

Qi²,

Sun³

2021

Ann Palliat Med

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“…The simulated gastric fluid consisted of HCl (0.045 mol/l) and pepsin (10 g/l), while the simulated intestinal fluid consisted of trypsin (10 g/l) and KH 2 PO 4 (6.8 g/l), and the pH was adjusted to 6.8 with 0.1 mol/l NaOH. The simulated colonic fluid was prepared as reported previously (Li et al, 2019). The feces were collected from three volunteers who had not taken any antibiotics in the past 6 months.…”

Section: Stability Of Tp2-fluorescein Isothiocyanate In Gastrointestimentioning

confidence: 99%

“…The pooled fecal sample (in a portion of 1:1:1 from each volunteer) was suspended in sterile normal saline with the addition of sterile glycerol at a ratio of 2:7:1 (g/ml/ml). Then the fecal supernatant was obtained by centrifugation at 4,000 × g for 5 min at 4°C, and stored at −80°C until subsequent use (Li et al, 2019). During the experiment, the fecal supernatant and the nutrient medium were mixed 1:5 and used as simulated colonic fluid.…”

Section: Stability Of Tp2-fluorescein Isothiocyanate In Gastrointestimentioning

confidence: 99%

“…(A) Colon adhesion; (B) ulcer degree; (C) ulcer incidence. Compared to the DNBS group, the difference was statistically significant, *p < 0.05, **p < 0.01. confirm degradation behavior of TP2 and the role of microbiota on the digestion of TP2, we established the simulated gastric fluid, simulated intestinal fluid, and simulated colonic fluid with fecal microbiota, as described previously (Li et al, 2019).…”

Section: In Vitro Digestion Of Tp2-fluorescein Isothiocyanatementioning

confidence: 99%

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Capsular Polysaccharide From Bacteroides fragilis Protects Against Ulcerative Colitis in an Undegraded Form

Zheng

Luo

Kuang³

et al. 2020

Front. Pharmacol.

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The prominent human symbiont Bacteroides fragilis protects animals from intestinal diseases, such as ulcerative colitis, and its capsular polysaccharide plays a key role in reducing inflammation. B. fragilis strain ZY-312 was isolated from the feces of a healthy breast-fed infant, and the zwitterionic capsular polysaccharide zwitterionic polysaccharide, TP2, was extracted. In rats with 2,4-dinitrobenzenesulfonic acid (DNBS)-induced enteritis, TP2 at an optimal dose of 2.5 mg/kg could significantly alleviate enteritis and reduced the degree of intestinal adhesions, the intestinal ulcer area, and the incidence of ulcers in rats. To understand the underlying mechanism, TP2 was labeled with Fluorescein isothiocyanate and orally administered at a dose of 2.5 mg/kg in rats. TP2 was mainly distributed in the cecum and colorectum, but it was not detected in the blood and other organs except that a compound with a molecular weight greater than that of TP2-FITC was found in liver tissue. During the absorption, distribution, metabolism, and excretion, TP2 was indigestible. These results were further confirmed by investigation in the simulated gastric, intestinal fluid, and colonic fluid with fecal microbiota in vitro, where TP2 remained unaltered at different time points. Furthermore, flora composition was analyzed in simulated colonic fluid with TP2 added and it was found that TP2 increased the abundance of Faecalibacterium, Enterococcus romboutsia, and Ruminococcaceae, whereas the abundance of the phylum Proteobacteria represented by Sutterella, Desulfovibrio, and Enterobacteriaceae was decreased. However, the amount of short-chain fatty acids in the simulated colonic fluid was not changed by intestinal flora post-TP2 addition. In conclusion, these findings confirmed that TP2, a capsular polysaccharide of B. fragilis, protects against ulcerative colitis in an undegraded form.

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“…The D. officinale-derived herb is known as "Tiepi Fengdou", and the international medical plant community refers to this herb as a "medicinal giant panda". D. officinale contains abundant polysaccharides, dendrobium alkaloids, flavonoids, and other bioactive substances, which are beneficial to human stomach health, exhibiting such activities as clearing heat and toxic material, enhancing immunity, reducing blood sugar concentration, and delaying aging, and this plant is recognized as a high-end health care product (Wei et al, 2016;Tang et al, 2017;Li et al, 2019). D. officinale has high medicinal value and is a valuable plant resource for this traditional Chinese medicine.…”

Section: Introductionmentioning

confidence: 99%

Development, Identification, and Application of a Germplasm Specific SCAR Marker for Dendrobium officinale Kimura et Migo

et al. 2021

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The stems of Dendrobium officinale have been used as a rare and valuable Chinese tonic medicine, known as “Tiepi Fengdou”, since the Qing dynasty. Because of the increased market demand and continued exploitation of this plant, the reserves of wild D. officinale resources have been depleted, and D. officinale products on the market are being increasingly adulterated. Such changes have strongly affected the sustainable utilization of this valuable medicinal plant resource and the development of related industries. In this study, a species-specific DNA marker was developed for the rapid and accurate authentication of D. officinale. In total, 36 start codon-targeted (SCoT) polymorphism primers were screened in 36 definite Dendrobium species, and a distinct species-specific DNA amplicon (SCoT13-215) for D. officinale was obtained. After the sequence was cloned and sequenced, a sequence-characterized amplified region marker was developed (named SHF/SHR) and validated through PCR amplification of all 38 Dendrobium samples. The marker’s specificity for D. officinale was confirmed through the consistent amplification of a clear 197-bp band. This SCAR marker can be used to rapidly, effectively, and reliably identify D. officinale among various Dendrobium species and may play an important role in ensuring the quality of medicinal preparations and protecting the germplasm of this important medicinal species.

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Destiny of Dendrobium officinale Polysaccharide after Oral Administration: Indigestible and Nonabsorbing, Ends in Modulating Gut Microbiota

Cited by 114 publications

References 32 publications

Traditional Chinese medicine, liver fibrosis, intestinal flora: is there any connection?—a narrative review

Traditional Chinese medicine, liver fibrosis, intestinal flora: is there any connection?—a narrative review

Capsular Polysaccharide From Bacteroides fragilis Protects Against Ulcerative Colitis in an Undegraded Form

Development, Identification, and Application of a Germplasm Specific SCAR Marker for Dendrobium officinale Kimura et Migo

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