Destabilization of IncA and IncC plasmids by SGI1 and SGI2 type Salmonella genomic islands

Harmer, Christopher J.; Hamidian, Mohammad; Ambrose, Stephanie J.; Hall, Ruth M.

doi:10.1016/j.plasmid.2016.09.003

Cited by 35 publications

(72 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The presence of SGI1-HKL variants in different serovars of S. enterica (18) and in other species (P. mirabilis [19, 27; this study] and M. morganii [22]) shows that they can be horizontally transferred even if there is a deletion from within traN to within S009. It has already been reported that these genes are not essential for the mobilization of SGI1 in the presence of an IncA or IncC plasmid (6,7). The variant SGI1-LK1, which harbors a larger deletion from within traN to within S013, is still transferred into E. coli, and the transfer rate of SGI1-LK1 was similar to those described previously (4).…”

Section: Sgi1-lk Variants In P Mirabilis and Their Evolutionsupporting

confidence: 79%

“…1A). This deletion does not abolish transfer (4,6). However, the class 1 integrons of these variants are at the same position as in SGI1, suggesting that this alteration occurred after the acquisition of the class 1 integron.…”

mentioning

confidence: 87%

“…SGI1-LK1 is mobilizable by an IncC plasmid. The standard HKL deletion (traN to S009) does not affect mobilization (6), nor does a longer deletion (traN to S0012) that inactivates or deletes all three tra genes (4). To determine whether the extended deletion from within traN to within S013 had an impact on the mobilization of SGI1-LK1 by IncC plasmids, mobilization assays were performed.…”

Section: Sgi1-lk Variants In P Mirabilis and Their Evolutionmentioning

confidence: 99%

“…They have been found so far in several Salmonella enterica serovars, Proteus mirabilis (1), and, more recently, Morganella morganii, Providencia stuartii, and Escherichia coli (2). SGI1 and its variants are inserted at the 3=-end of the chromosomal trmE (formerly thdF) gene and are mobilized specifically by IncA and IncC plasmids (1,(3)(4)(5)(6)(7). SGI1, the first variant identified, was found in Salmonella enterica serovar Typhimurium and is made up of a 27.4-kb backbone containing 28 open reading frames (ORFs) from int SGI1 (S001) to resG (S027) and S044 and a 15-kb complex class 1 integron inserted upstream of the resG gene and flanked by a 5-bp duplication (ACTTG) (8).…”

mentioning

confidence: 99%

See 3 more Smart Citations

Two New SGI1-LK Variants Found in Proteus mirabilis and Evolution of the SGI1-HKL Group of Salmonella Genomic Islands

Curraize

Siébor

Varin

et al. 2020

mSphere

Self Cite

View full text Add to dashboard Cite

Integrative mobilizable elements belonging to the SGI1-H, -K, and -L Salmonella genomic island 1 (SGI1) variant groups are distinguished by the presence of an alteration in the backbone (IS1359 replaces 2.8 kb of the backbone extending from within traN [S005] to within S009). Members of this SGI1-HKL group have been found in Salmonella enterica serovars and in Proteus mirabilis. Two novel variants from this group, designated SGI1-LK1 and SGI1-LK2, were found in the draft genomes of antibiotic-resistant P. mirabilis isolates from two French hospitals. Both variants can be derived from SGI1-PmGUE, a configuration found previously in another P. mirabilis isolate from France. SGI1-LK1 could arise via an IS26-mediated inversion in the complex class 1 integron that duplicated the IS26 element and the target site in IS6100. SGI1-LK1 also has a larger 8.59-kb backbone deletion extending from traN to within S013 and removing traG and traH. However, SGI1-LK1 was mobilized by an IncC plasmid. SGI1-LK2 can be derived from a hypothetical progenitor, SGI1-LK0, that is related to SGI1-PmGUE but lacks the aphA1 gene and one copy of IS26. The integron of SGI1-LK2 could arise via deletion of DNA adjacent to an IS26 and a deletion occurring via homologous recombination between duplicated copies of part of the integron 3=-conserved segment. SGI1-K can also be derived from SGI1-LK0. This would involve an IS26-mediated deletion and an inversion via homologous recombination of a segment between inversely oriented IS26s. Similar events can explain the configuration of the integrons in other SGI1-LK variants. IMPORTANCE Members of the SGI1-HKL subgroup of SGI1-type integrative mobilizable elements have a characteristic alteration in their backbone. They are widely distributed among multiply antibiotic-resistant Salmonella enterica serovars and Proteus mirabilis isolates. The SGI1-K type, found in the globally disseminated multiply antibiotic-resistant Salmonella enterica serovar Kentucky clone ST198 (sequence type 198), and various configurations in the original SGI1-LK group, found in other multiresistant S. enterica serovars and Proteus mirabilis isolates, have complex and highly plastic resistance regions due to the presence of IS26. However, how these complex forms arose and the relationships between them had not been analyzed. Here, a hypothetical progenitor, SGI1-LK0, that can be formed from the simpler SGI1-H is proposed, and the pathways to the formation of new variants, SGI1-LK1 and SGI1-LK2, found in P. mirabilis and other reported configurations via homologous recombination and IS26-mediated events are proposed. This led to a better understanding of the evolution of the SGI1-HKL group.

show abstract

Section: Sgi1-lk Variants In P Mirabilis and Their Evolutionsupporting

confidence: 79%

mentioning

confidence: 87%

Section: Sgi1-lk Variants In P Mirabilis and Their Evolutionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Two New SGI1-LK Variants Found in Proteus mirabilis and Evolution of the SGI1-HKL Group of Salmonella Genomic Islands

Curraize

Siébor

Varin

et al. 2020

mSphere

Self Cite

View full text Add to dashboard Cite

show abstract

“…The significant levels of amino acid identity between the TrbJ, TrbK and TrbL proteins of GIsul2 and those found in IncP plasmids suggests that GIsul2 may be mobilized by IncP plasmids in much the same way that IncA and IncC plasmids are able to mobilize SGI1 and SGI2 type Salmonella genomic islands (Hall, 2010;Harmer et al, 2016a;Douard et al, 2010;Doublet et al, 2005;Kiss et al, 2015;Carraro et al, 2014a). This warrants further investigation.…”

Section: Distribution Of Gisul2mentioning

confidence: 92%

pIP40a, a type 1 IncC plasmid from 1969 carries the integrative element GI sul2 and a novel class II mercury resistance transposon

Harmer

Hamidian

Hall

2017

Plasmid

Self Cite

View full text Add to dashboard Cite

The 167.5 kb sequence of the conjugative IncC plasmid pIP40a, isolated from a Pseudomonas aeruginosa in 1969, was analysed. pIP40a confers resistance to kanamycin, neomycin, ampicillin, sulphonamides and mercuric ions, and several insertions in a type 1 IncC backbone were found, including copies of IS3, Tn1000 and a novel mercury resistance transposon, Tn6182. The antibiotic resistance genes were in two locations. Tn6023, conAntibiotic resistance Transposon taining the aphA1 kanamycin and neomycin resistance gene, is in a partial copy of Tn1/Tn2/Tn3 (bla TEM, ampicillin resistance) in the kfrA gene, and the sul2 sulphonamide resistance gene is in the integrative element GIsul2 in the position of ARI-B islands. The 11.5 kb class II transposon Tn6182 is only distantly related to other class II transposons, with at most 33% identity between the TnpA of Tn6182 and TnpA of other group members. In addition, the inverted repeats are 37 bp rather than 38 bp, and the likely resolution enzyme is a tyrosine recombinase (TnpI). Re-annotation of GIsul2 revealed genes predicted to confer resistance to arsenate and arsenite, but resistance was not detected. The location of GIsul2 confirms it as the progenitor of the ARI-B configurations seen in many IncC plasmids isolated more recently. However, GIsul2 has integrated at the same site in type 1 and type 2 IncC plasmids, indicating that it targets this site. Analysis of the distribution of GIsul2 revealed that it in addition to its chromosomal integration site at the 3′-end of the guaA gene, it has also integrated into other plasmids, increasing its mobility.

show abstract

New insights regarding Acinetobacter genomic island-related elements

Siébor

Neuwirth

2020

International Journal of Antimicrobial Agents

View full text Add to dashboard Cite

Destabilization of IncA and IncC plasmids by SGI1 and SGI2 type Salmonella genomic islands

Cited by 35 publications

References 26 publications

Two New SGI1-LK Variants Found in Proteus mirabilis and Evolution of the SGI1-HKL Group of Salmonella Genomic Islands

Two New SGI1-LK Variants Found in Proteus mirabilis and Evolution of the SGI1-HKL Group of Salmonella Genomic Islands

pIP40a, a type 1 IncC plasmid from 1969 carries the integrative element GI sul2 and a novel class II mercury resistance transposon

New insights regarding Acinetobacter genomic island-related elements

Contact Info

Product

Resources

About