Desoxyribonucleic acid synthesis in the lymphocytes of patients with malignant disease

Crowther, Derek; Fairley, G. Hamilton; Sewell, R. L.

doi:10.1016/0014-2964(67)90026-6

Cited by 15 publications

(6 citation statements)

References 3 publications

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“…There was only a small rise in labeling index to 0.14 % after immunization, but considerable increases in the uptake of uridine and leucine were seen. (17,18). It has been suggested that these cells form part of a cellular immunological reaction which is present in patients with this disease.…”

Section: Studies Of the Secondary Immunementioning

confidence: 99%

“…In addition to normal subjects, patients with Hodgkin's disease in remission were also included because we have previously shown changes in the lymphoid cell population in the active phase of this disease similar to those described here, but which return to normal when the disease is in remission (17,18). 1 Patients with carcinoma were also studied to determine whether their cellular response to antigenic stimulation was similar to normal subjects.…”

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confidence: 99%

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Lymphoid Cellular Responses in the Blood After Immunization in Man

Crowther¹,

Fairley²,

Sewell³

1969

The Journal of Experimental Medicine

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A combined morphological and metabolic study has been made of the lymphoid cells in the blood during the immune response in man. Similar changes were observed in both primary and secondary responses to a number of different microbial antigens. The cellular response involved an increase in numbers of three types of cell; hyperbasophilic medium lymphocytes, plasma cells, and large lymphoid cells. The large lymphoid cells were about 20 µ in diameter with large nuclei, prominent nucleoli, and an intensely basophilic cytoplasm with numerous polyribosomes. About 30% of these cells were in the DNA synthetic phase of cell growth. Electron microscopy has shown that many of the basophilic medium-sized cells have sufficient well-organized endoplasmic reticulum to be included in the plasma cell series. The hyperbasophilic cells labeled more heavily with tritiated uridine and tritiated leucine than the normal small and medium lymphocytes from the peripheral blood of patients not under antigenic stimulation. The evidence in this paper supports the argument that the atypical mononuclear cells first described by Türk and others in the blood of patients with infections are immunoblasts, plasma cells, and other reactive lymphoid cells representing a circulating population of lymphoid cells derived from lymphoid tissue responding to antigenic stimulation. The presence of such cells may be a valuable indication that an immunological reaction is in progress when direct proof is lacking.

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Section: Studies Of the Secondary Immunementioning

confidence: 99%

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confidence: 99%

Lymphoid Cellular Responses in the Blood After Immunization in Man

Crowther¹,

Fairley²,

Sewell³

1969

The Journal of Experimental Medicine

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“…However, we cannot exclude the presence of tumour cells in blood in these cases, because the MBCB test may have been negative due to low numbers of B cells, low surface Ig density or presence of an unidentified T-cell lymphoma. Proliferating blood lymphocytes have been shown not only in patients with chronic lymphocytic leukaemia (27,28), non-Hodgkin's lymphomas (1 l), Hodgkin's disease (29,30) and multiple myeloma (15), but also in patients without malignant haematopoietic disorders (29,31). The S-phase fraction of mononuclear cells may only to a limited extent reflect the MBCB-positive tumour cell population, since the proportion of monocytes, T cells and polyclonal B cells varies from case to case and the fraction of proliferating cells among these populations is not known.…”

Section: Discussionmentioning

confidence: 99%

Fraction of S‐phase cells in blood mononuclear cells in non‐Hodgkin's lymphomas—Correlation with clinical features and prognosis

Lindh

Jonsson

Lenner

et al. 1989

European J of Haematology

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A consecutive material of 111 untreated patients with non‐Hodgkin's lymphoma was studied with respect to fraction of S‐phase cells in blood mononuclear cells in relation to presence of monoclonal B cells in blood (MBCB). Fraction of S‐phase cells was determined by flow cytometry and estimation of MBCB was performed by kap‐pa:lambda analysis. The fraction of S‐phase cells was significantly higher (p < 0.001) in MBCB‐positive cases (median 1.2%) than in the MBCB‐negative (median 0.7%). MBCB‐positive patients with S‐phase values ≥ 1.5% had a less favourable prognosis compared to those with < 1.5% cells in S‐phase (p = 0.01). In a Cox multiparameter analysis, advanced clinical stage, high‐grade morphology and high fraction of S‐phase cells in blood in MBCB‐positive cases were independent, statistically significant, negative prognostic indicators. The results indicate that an elevated S‐phase value in blood in non‐Hodgkin's lymphoma constitutes a negative prognostic factor, probably reflecting proliferating tumour cells in blood.

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“…Control persons consisted of 16 healthy individuals (HI) (mean age 31 ± 6 years) working at the neurological department and 20 patients suffering from other neurological diseases (OND) including: epilepsy e.c.i. (4), cerebrovascular disease (7), normal pressure hydrocephalus (2). trigeminal neuralgia (1).…”

Section: Methodsmentioning

confidence: 99%

“…Increased desoxyribonucleic acid (DNA) synthesis has been observed in blood lym phocytes of patients with Hodgkin's disease [4,5], with other malignant diseases and in patients suffering from bacterial or viral in fections [4], Increased numbers of DNA-synthesizing cells have also been found in pa tients suffering from neurologic disorders, i.e., the Guillain-Barre syndrome, acute dis seminated encephalomyelitis and carcinoma tous neuropathy [3].…”

Section: Introductionmentioning

confidence: 99%

Spontaneous and Mitogen-induced Activity of Lymphocytes of Different Density in Multiple Sclerosis

Brinkman¹,

Nillesen²,

Hommes³

et al. 1982

Eur Neurol

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Some immunological parameters of patients with multiple sclerosis (MS), of patients with other neurological diseases and of healthy volunteers were compared. No significant differences between these groups of persons were found in the number of leukocytes, granulocytes or lymphocytes per milliliter of peripheral blood. A density distribution profile of the peripheral blood mononuclear cells revealed a relatively increased number of low density cells (≤ 1.069 g/cm³) and a relatively decreased number of cells with higher density (1.069–1.073 g/cm³) in MS patients as compared to healthy controls. The spontaneous activity and the pokeweed mitogen-induced activity of the different cell subfractions, separated according to density, appeared to be normal in MS patients. The results suggest a continuous stimulation of the immune system of MS patients resulting in a shift of cells towards lower density. This change was not related to disease duration or severity.

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Desoxyribonucleic acid synthesis in the lymphocytes of patients with malignant disease

Cited by 15 publications

References 3 publications

Lymphoid Cellular Responses in the Blood After Immunization in Man

Lymphoid Cellular Responses in the Blood After Immunization in Man

Fraction of S‐phase cells in blood mononuclear cells in non‐Hodgkin's lymphomas—Correlation with clinical features and prognosis

Spontaneous and Mitogen-induced Activity of Lymphocytes of Different Density in Multiple Sclerosis

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