Desorption electrospray ionization-high resolution mass spectrometry for the screening of veterinary drugs in cross-contaminated feedstuffs

Seró, Raquel; Núñez, Óscar; Bosch, Jaume; Grases, J.M.; Rodrí­guez, Pilar Rodriguez; Moyano, Encarnación; Galcerán, M.T.

doi:10.1007/s00216-015-8899-4

Cited by 16 publications

(3 citation statements)

References 34 publications

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“…Mixer overfilling and deposits from the mixer, as well as, the sequence of ingredient addition and mixing time may influence the product (Stark and Jones 2009;Rocha et al 2015;Huss et al 2018;Martínez et al 2018). Additionally, the antibiotic residue found in feed labeled as non-medicated can be attributed to errors in labeling or batch to batch cross-contamination (Stolker et al 2013;Seró et al 2015;Filippitzi et al 2016).…”

Section: Residues Found In Batch-to-batch Cleaning or Flushing Samplesmentioning

confidence: 99%

Chemical assessment of mycotoxin contaminants and veterinary residues in Costa Rican animal feed

et al. 2019

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Background: When present in animal feedstuff, mycotoxins contaminants and antibiotic residues can have negative implications for animal production and Public Health, including the transmission of carcinogenic compounds and the selection of antibiotic resistant bacteria, respectively. So far there are no available methods in which both mycotoxins and antibiotic residues are analyzed using a parallel extraction approach. To address this issue, we developed a LC/MS methodology with high sensitivity (0.005 to 6.42 and 24.55 to 132.73 μg kg − 1 for mycotoxins and antimicrobials) and specificity (unique target ion mass/charge) that allows the detection of 26 mycotoxins and 23 antibiotic residues in animal feedstuff and validated it through the determination of these analytes in 294 animal feed and feed ingredient samples in the framework of a country-wide surveillance program. Two hundred and five of these samples were analyzed for mycotoxins and 89 for antibiotics. Findings: Fumonisin was the most frequently toxin found, with FB 1 and FB 2 presenting prevalences of 50 and 52% and maximum concentrations of 14,927.61 and 8646.67 μg kg − 1 , respectively. Other toxins, including diacetoxyscirpenol n = 4/101 (3.96%), fusarenon-X n = 2/101 (1.98%), citrinin n = 2 (1.98%), and patulin n = 1 (0.99%) were rarely found. Toxicologically relevant concentrations of toxin metabolites, such as HT-2 (6.38-485.49 μg kg − 1) and 3−/15acetoxydeoxynivalenol (877.89-3236.56/5.44-1685.3 μg kg − 1), were also found. Few samples exceeded threshold mycotoxin concentrations defined in current EU guidelines. Dairy cattle and swine feeds included the higher number of samples exceeding guideline values (n = 6 and n = 5, respectively). From the total of samples analysed for antibiotics, 7.7% (n = 7/89) were classified as medicated for poultry and pigs. Unexpectedly, 57% of these medicated samples contained no detectable antibiotics (n = 4/7). The remaining 43% of the samples (n = 3/7) presented inconsistencies regarding the concentration of analytes declared on the labels or the antibiotics found. Likewise 74.6% (n = 50/67) of the non-medicated feed samples analyzed had antibiotic residues. Additionally, we analyzed commercial monensin standards for purity and evaluate batch-to-batch flushing feed industry practices. Conclusions: Herein we report the results for a year-wide analysis for mycotoxins and antibiotics in feed samples. Mycotoxins, several metabolites, and the occurrence of these emerging contaminants were evaluated and antibiotic residues in non-medicated feed samples were found using a targeted MS-based LC approach. This validated multianalyte method is expected to facilitate the monitoring and surveillance of contaminants, from natural and anthropogenic origin, in animal feed.

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Section: Residues Found In Batch-to-batch Cleaning or Flushing Samplesmentioning

confidence: 99%

Chemical assessment of mycotoxin contaminants and veterinary residues in Costa Rican animal feed

et al. 2019

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“…In order not to duplicate the data, Table 1 does not contain results included in a review a few years earlier published by Morelato et al [9]. DESI-MS method has also proved its potential in many other non-forensic applications such as food control analysis of multiple pesticides on different food surfaces [40], analysis of drugs in wastewater [41], veterinary drugs in feedstuffs [42], lubricant oil additives [43] and clinical diagnostics [21,46]. Research shows great DESI-MS potential in the analysis of lipids, proteins, protein complexes, carbohydrates, and oligonucleotides, specimens from industrial polymers to small organic molecules [44].…”

Section: Applicationmentioning

confidence: 99%

DESI-MS analysis of human fluids and tissues for forensic applications

Wójtowicz

Wietecha‐Posłuszny

2019

Appl. Phys. A

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Desorption electrospray ionization mass spectrometry (DESI-MS) is an ambient ionization technique which application in forensic chemistry appears to be very promising. The technique enables rapid in situ analyses without the need of elaborate sample preparation. Nowadays, many DESI-MS applications in forensics, medical diagnostics and food control have been already reported in the literature. The study summarizes the latest DESI-MS forensic applications in the analysis of biological fluids and tissues, i.e., fingerprints, skin, urine and blood, in which the interest has grown during the recent years. In the publication presented below, the use of blood samples in dried blood spots is also included. The analysis reported covers mainly the determination of medicines and illicit drugs content in biological fluids and tissues, where great DESI-MS potential in screening and drugs identification was recognized. Furthermore, the examples of explosives and condoms components detection are also present. Abbreviations ACN Acetonitrile DBS Dried blood spots DESI-MS Desorption electrospray ionization mass spectrometry DMF N,N-dimethylformamide EtOH Ethanol GC-MS Gas chromatography-mass spectrometry GSR Gunshot residues LC-MS/MS Liquid chromatography tandem mass spectrometry LOD Limit of detection LOQ Limit of quantification MeOH Methanol OTC drugs Over-the-counter drugs PMMA Poly(methyl methacrylate) PTFE Polytetrafluoroethylene RDX Trinitrohexahydro-1,3,5-triazine TFME Thin-film microextraction TLME Thin liquid membrane extraction TLC Thin-layer chromatography TNT 2,4,6-Trinitrotoluene QuEChERS extraction Quick, easy, cheap, effective, rugged, and safe extraction

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“…In addition, minimal training is required. Several analytical methods have been described in the literature for the analysis of antiparasitic agents in feed [ 23 , 24 ], and in animal products and tissues, such as milk, liver, muscle, and egg [ 25 , 26 , 27 , 28 , 29 , 30 ]. However, most studies of anthelmintic and antiprotozoal residues have focused on livestock products and only a few studies report on mixtures of 20 or more types of drugs [ 27 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

Determination of Anthelmintic and Antiprotozoal Drug Residues in Fish Using Liquid Chromatography-Tandem Mass Spectrometry

Kim

Park

et al. 2021

Molecules

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The objective of this study is to develop a comprehensive and simple method for the simultaneous determination of anthelmintic and antiprotozoal drug residues in fish. For sample preparation, we used the “quick, easy, cheap, effective, rugged, and safe” (QuEChERS) method with a simple modification. The sample was extracted with water and 1% formic acid in acetonitrile/methanol (MeCN/MeOH) (95:5, v/v), followed by phase separation (salting out) with MgSO4 and NaCl (4:1, w/w). After centrifugation, an aliquot of the extract was purified by dispersive solid-phase extraction (d-SPE) prior to liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. The method was validated at three concentration levels for all matrices, in accordance with the Codex guidelines (CAC/GL-71). Quantitative analysis was performed using the method of matrix-matched calibration. The recoveries were between 60.6% and 119.9%, with coefficients of variation (CV) <30% for all matrices. The limit of quantitation (LOQ) of the method ranged from 0.02 μg kg−1 to 4.8 μg kg−1 for all matrices. This comprehensive method can be used for the investigation of both anthelmintic and antiprotozoal drugs belonging to different chemical families in fishery products.

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Desorption electrospray ionization-high resolution mass spectrometry for the screening of veterinary drugs in cross-contaminated feedstuffs

Cited by 16 publications

References 34 publications

Chemical assessment of mycotoxin contaminants and veterinary residues in Costa Rican animal feed

Chemical assessment of mycotoxin contaminants and veterinary residues in Costa Rican animal feed

DESI-MS analysis of human fluids and tissues for forensic applications

Determination of Anthelmintic and Antiprotozoal Drug Residues in Fish Using Liquid Chromatography-Tandem Mass Spectrometry

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