Desmoplakin is essential in epidermal sheet formation

Vasioukhin, Valeri; Bowers, Ethan; Bauer, Christoph; Degenstein, Linda; Fuchs, Elaine

doi:10.1038/ncb1201-1076

Cited by 277 publications

(344 citation statements)

References 36 publications

Supporting

Mentioning

308

Contrasting

Unclassified

Order By: Relevance

“…DSP KO embryos did not survive beyond embryonic day 6.5 and displayed severe defects in tissue architecture, shaping of the embryo, and in anchoring keratin filaments to desmosomes (Gallicano et al 1998). In an epidermisspecific DSP KO, intercellular separations were observed as expected (Vasioukhin et al 2001). Surprisingly, desmosome number was unaltered although the lack of keratin association compromised their function.…”

Section: Evidence From Mouse Models and Human Diseasesmentioning

confidence: 91%

“…Desmosomes have been recognized early on as important sites for KIF formation and organization in vivo (Jackson et al 1980;Bologna et al 1986;Schwarz et al 2015), and genetic deletion of DSP can cause extensive reorganization or collapse of KIF, depending on the cell type (Gallicano et al 1998;Vasioukhin et al 2001;Sumigray and Lechler 2012). Moreover, DSP and DSG1 mutations can give rise to SPPK (OMIM #612908, #148700, respectively), a hyperkeratotic skin condition with fewer or smaller desmosomes in the suprabasal epidermis and perinuclear accumulation of KIF in DSP-associated SPPK (Wan et al 2004).…”

Section: Interdependence Of Keratins and Desmosomesmentioning

confidence: 99%

See 1 more Smart Citation

Desmosomes and Intermediate Filaments: Their Consequences for Tissue Mechanics

Hatzfeld

Keil

Magin

2017

Cold Spring Harb Perspect Biol

113

View full text Add to dashboard Cite

Adherens junctions (AJs) and desmosomes connect the actin and keratin filament networks of adjacent cells into a mechanical unit. Whereas AJs function in mechanosensing and in transducing mechanical forces between the plasma membrane and the actomyosin cytoskeleton, desmosomes and intermediate filaments (IFs) provide mechanical stability required to maintain tissue architecture and integrity when the tissues are exposed to mechanical stress. Desmosomes are essential for stable intercellular cohesion, whereas keratins determine cell mechanics but are not involved in generating tension. Here, we summarize the current knowledge of the role of IFs and desmosomes in tissue mechanics and discuss whether the desmosome-keratin scaffold might be actively involved in mechanosensing and in the conversion of chemical signals into mechanical strength.

show abstract

Section: Evidence From Mouse Models and Human Diseasesmentioning

confidence: 91%

Section: Interdependence Of Keratins and Desmosomesmentioning

confidence: 99%

Desmosomes and Intermediate Filaments: Their Consequences for Tissue Mechanics

Hatzfeld

Keil

Magin

2017

Cold Spring Harb Perspect Biol

113

View full text Add to dashboard Cite

show abstract

“…Conditional ablation of desmoplakin in the mouse epidermis reveals a role of the protein in formation and maintenance of epithelial sheets (Vasioukhin et al, 2001). Disassembly of desmosomes at the onset of cytokine-induced EMT is regulated by Slug, a zinc-finger transcriptional repressor that inhibits the desmoplakin and desmoglein genes to facilitate progression of EMT (Savagner et al, 1997).…”

Section: Disintegration Of Cell-cell Adhesions and Loss Of Epithelialmentioning

confidence: 99%

TGF-β and epithelial-to-mesenchymal transitions

2005

View full text Add to dashboard Cite

Remarkable phenotype plasticity of epithelial cells underlies morphogenesis, epithelial repair and tumor invasiveness. Detailed understanding of the contextual cues and molecular mediators that control epithelial plasticity will be required in order to develop viable therapeutic approaches targeting epithelial-to-mesenchymal transition (EMT), an advanced manifestation of epithelial plasticity. Members of the transforming growth factor (TGF-b) family of growth factors can initiate and maintain EMT in a variety of biological systems and pathophysiological context by activating major signaling pathways and transcriptional regulators integrated in extensive signaling networks. Here we will review the distinct physiological contexts of EMT and the underlying molecular signaling networks controlled by TGF-b.

show abstract

“…DP has been studied recently in great detail using homologous recombination to knockout gene function, both tissue specifically and globally. DP has also been knocked down in endothelial cells using siRNA Vasioukhin et al, 2001;Zhou et al, 2004). Each of those investigations clearly demonstrated the importance of DP, especially in the three-dimensional formation of developing epidermis, neurectoderm, heart, and capillaries.…”

Section: Fig 1 Kaz Colocalizes To the Spindle Apparatusmentioning

confidence: 97%

Dynamics and unexpected localization of the plakin binding protein, kazrin, in mouse eggs and early embryos

Gallicano

Foshay

Pengetnze

et al. 2005

Developmental Dynamics

View full text Add to dashboard Cite

The cell uses the cytoskeleton in virtually every aspect of cell survival and function. One primary function of the cytoskeleton is to connect to and stabilize intercellular junctions. To accomplish this task, microtubules, actin filaments, and intermediate filaments utilize cytolinker proteins, which physically bind the cytoskeletal filament to the core proteins of the adhesion junction. The plakin family of linker proteins have been in the spotlight recently as critical components for embryo survival and, when mutated, the cause of diseases such as muscular dystrophy and cardiomyopathies. Here, we reveal the dynamics of a recently discovered plakin binding protein, kazrin (kaz), during early mouse development. Kaz was originally found in adult tissues, primarily epidermis, linking periplakin to the plasma membrane and colocalizing with desmoplakin in desmosomes. Using reverse transcriptase-polymerase chain reaction, Western blots, and confocal microscopy, we found kaz in unfertilized eggs associated with the spindle apparatus and cytoskeletal sheets. As quickly as 5 min after egg activation, kaz relocates to a diffuse peri-spindle position, followed 20 -30 min later by clear localization to the presumptive cytokinetic ring. Before the blastocyst stage of development, kaz associates with the nuclear matrix in a cell cycle-dependent manner, and also associates with the cytoplasmic actin cytoskeleton. After blastocyst formation, kaz localization and potential function(s) become highly complex as it is found associating with cell-cell junctions, the cytoskeleton, and nucleus. Postimplantation stages of development reveal that kaz retains a multifunctional, tissue-specific role as it is detected at diverse locations in various embryonic tissue types.

show abstract

Desmoplakin is essential in epidermal sheet formation

Cited by 277 publications

References 36 publications

Desmosomes and Intermediate Filaments: Their Consequences for Tissue Mechanics

Desmosomes and Intermediate Filaments: Their Consequences for Tissue Mechanics

TGF-β and epithelial-to-mesenchymal transitions

Dynamics and unexpected localization of the plakin binding protein, kazrin, in mouse eggs and early embryos

Contact Info

Product

Resources

About