Desmin deficiency is not sufficient to prevent corneal fibrosis

Pietraszkiewicz, Alexandra; Hampton, Christopher; Caplash, Sonny; Le, Lei; Capetanaki, Yassemi; Tadvalkar, Gauri; Pal‐Ghosh, Sonali; Stepp, Mary Ann; Bargagna‐Mohan, Paola; Mohan, Royce

doi:10.1016/j.exer.2018.12.019

Cited by 3 publications

(3 citation statements)

References 31 publications

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Section: Resultssupporting

confidence: 91%

“…At late time points PTI, we detected myofibroblasts (Acta2-positive cells) that expressed high levels of Des and Igfbp5 compared to the IL6 + fibroblasts cells ( Supplementary Fig. 11f), which is consistent with previous reports 63,64 . Three additional subpopulations of fibroblasts expressed distinct sets of genes (clusters F10-F12, Supplementary Fig.…”

Section: Fibroblasts Along the Development Of Lesions And Tumorssupporting

confidence: 91%

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Single-cell transcriptomes of pancreatic preinvasive lesions and cancer reveal acinar metaplastic cells’ heterogeneity

Schlesinger¹,

Yosefov-Levi²,

et al. 2020

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Acinar metaplasia is an initial step in a series of events that can lead to pancreatic cancer. Here we perform single-cell RNA-sequencing of mouse pancreas during the progression from preinvasive stages to tumor formation. Using a reporter gene, we identify metaplastic cells that originated from acinar cells and express two transcription factors, Onecut2 and Foxq1. Further analyses of metaplastic acinar cell heterogeneity define six acinar metaplastic cell types and states, including stomach-specific cell types. Localization of metaplastic cell types and mixture of different metaplastic cell types in the same pre-malignant lesion is shown. Finally, single-cell transcriptome analyses of tumor-associated stromal, immune, endothelial and fibroblast cells identify signals that may support tumor development, as well as the recruitment and education of immune cells. Our findings are consistent with the early, premalignant formation of an immunosuppressive environment mediated by interactions between acinar metaplastic cells and other cells in the microenvironment.

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Section: Resultssupporting

confidence: 91%

Section: Fibroblasts Along the Development Of Lesions And Tumorssupporting

confidence: 91%

Single-cell transcriptomes of pancreatic preinvasive lesions and cancer reveal acinar metaplastic cells’ heterogeneity

Schlesinger¹,

Yosefov-Levi²,

et al. 2020

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“…98,117,[148][149][150][151] In addition to collagens, myofibroblasts express increased amounts of intermediate filaments, vimentin, and desmin. 152 Finally, myofibroblasts release TGFβ after supplies from epithelium have ceased. 124,140 Continuous release of TGFβ can be helpful but may become hurtful for long-term healing because it allows a severe injury to continue healing, but it allows the excessive and prolonged formation of the myofibroblast in early wound healing stages.…”

Section: Basics Of Corneal Stromal Wound Healingmentioning

confidence: 99%

Mechanistic understanding and potential targets for mustard gas keratopathy

Sinha¹

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Sulfur mustard gas (SM), a vesicating and alkylating agent, has been used as a warfare agent since World War I and recently in Syria. SM exposure causes severe corneal injury, pain, and irreversible blindness. The cornea provides twothirds of the eye's light refraction vital for vision. Clinically, SM rapidly penetrates the cornea after exposure and causes a grade-3 or grade-4 clinical pathology called mustard gas keratopathy (MGK). MGK is characterized by ocular inflammation, loss of epithelial barrier, recurrent epithelial erosions, epithelialstromal separation, haze/fibrosis, and neovascularization in the cornea. Despite the high risk of SM for terrorism and mass obliteration, the pathophysiological mechanisms associated with MGK remain elusive. MGK-related corneal haze/fibrosis results from the transdifferentiation of keratocytes into pathological cellular phenotype called myofibroblasts that cause massive overproduction of collagen during wound healing. Keratocytes and collagens residing in the stroma play an essential role in maintaining corneal transparency and are regulated by cytokines and chemokines. The predominant collagens in the stroma are Type I, V, and VII. Additionally, a unique arrangement of collagen fibrils is required for transparency and corneal function. Collagen fibrils are narrow with uniform diameter and arranged with a high degree of lateral ordering. The mechanisms involving myofibroblast formation and collagen derangement after mustard gas exposure are unknown. Presently, a major knowledge gap is how SM exposure affects myofibroblast generation and collagen fibril arrangement in the wounded cornea. My dissertation fills this knowledge gap through successful testing of a novel central hypothesis that mustard gas exposure to the eye results in corneal haze/fibrosis by excessive production of myofibroblasts via TGFβ/SMAD signaling and alters the distinctive organization of collagen fibrils by regulating LOX activity via PI3K/MAPK signaling in the stroma. The formation of haze/fibrosis post SM exposure was tested using an in vivo rabbit model, in which live New Zealand White rabbits received SM vapor exposure localized to the eye. The goals of my PhD thesis were to characterize time-dependent changes in the stroma in live animals in situ employing eye examinations and state-of-the-art multimodal clinical ophthalmic imaging techniques in live rabbits with Stereo, Slit-lamp, HRT-RCM3, and Spectralis microscopy system. Fantes grading, fluorescein staining, Schirmer's tests, pachymetry, and applanation tonometry were conducted to measure levels of corneal haze, ocular surface healing, tears, corneal thickness, and intraocular pressure, respectively. H and E and PSR staining were used for histopathological cellular changes in the cornea. In vivo confocal and OCT imaging revealed significant changes in structural and morphological appearance of the cornea in vivo in SM-exposed rabbit corneas in a time-dependent manner compared to naive cornea (p [less than] 0.001 or p [less than] 0.0001). Also, SM-exposed eyes showed loss of corneal transparency characterized by increased stromal thickness (p [less than] 0.0001), debris, and light-scattering myofibroblasts or activated keratocytes, representing haze formation in the cornea. Naive eyes did not show any structural, cellular, and functional abnormalities. SM exposure showed increased central corneal thickness, ocular edema and tearing compared to the eyes of the naive (p [less than] 0.001 or p [less than] 0.0001). This study concluded that structural and cellular changes in primary corneal layers are early pathological events contributing to MGK in vivo. Next, I tested if TGFβ/SMAD is a major molecular mechanism for myofibroblast formation following SM exposure. Rabbit corneas exposed to SM had a time-dependent increase in [alpha]SMA, a maker for myofibroblast formation. Additionally, using an established in vitro model of primary human corneal fibroblasts (hCSF) treated with nitrogen mustard (NM), an analogous agent to SM, or NM+SMAD inhibitors, I verified my hypothesis that SMAD is a predominant pathway for myofibroblast formation in MGK. Primary hCSF treated with NM showed a significant time-dependent increase (p [less than] 0.05, p [less than] 0.01) in [alpha]SMA expression which was significantly decreased by SMAD inhibitors (p [less than] 0.05, p [less than] 0.01). This data strongly supported my notion that SMAD is a major pathway for myofibroblast formation in corneal stroma after mustard gas exposure. The final section of my thesis tested another novel postulate that lysyl oxidase (LOX) is a possible mechanism to cause changes in the collagen lamella. LOX activity regulates extracellular cross-linking; however, literature/knowledge on LOX function in the cornea is very limited. Thus, I tested if p38 and PI3K are major regulatory pathways. p38 and PI3K have shown to regulate steady state LOX mRNA in cardiac fibroblasts. An in vitro study using hCSF cells, NM, p38 inhibitor, and PI3K inhibitor concluded that (a) p38 follow the pattern similar to SMAD signaling, and (b) PI3K was directly related to LOX production. Subsequent research showed that p38 has a function correlative to SMAD function in the corneal epithelium. This finding was the first to identify the role of PI3K in the mustard gas exposed corneal stroma. In conclusion, my dissertation identified time-dependent in situ structural and cellular aberrations in the rabbit cornea in vivo after mustard gas exposure and remarkably improved mechanistic knowledge that will be instrumental in developing novel strategies and molecular targets for newer therapeutics to prevent and treat corneal mustard gas keratopathy. Additionally, it might help understanding corneal pathologies caused by other alkylating/vesicating agents.

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Corneal stromal wound healing: Major regulators and therapeutic targets

Kamil

Mohan

2021

The Ocular Surface

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Desmin deficiency is not sufficient to prevent corneal fibrosis

Cited by 3 publications

References 31 publications

Single-cell transcriptomes of pancreatic preinvasive lesions and cancer reveal acinar metaplastic cells’ heterogeneity

Single-cell transcriptomes of pancreatic preinvasive lesions and cancer reveal acinar metaplastic cells’ heterogeneity

Mechanistic understanding and potential targets for mustard gas keratopathy

Corneal stromal wound healing: Major regulators and therapeutic targets

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