Designing vascular supportive albumen-rich composite bioink for organ 3D printing

Liu, Suihong; Zhang, Haiguang; Hu, Qingxi; Shen, Zhipeng; Rana, Deepti; Ramalingam, Murugan

doi:10.1016/j.jmbbm.2020.103642

Cited by 40 publications

(36 citation statements)

References 44 publications

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“…Hydrogel solutions were prepared at room temperature by mixing 5% (w/v) SA and albumen with 5:1 volume ratio. 30…”

Section: Methodsmentioning

confidence: 99%

“…29 In previous studies, we found that the electrospinning scaffolds after blend rigid PCL and flexible TPU, could simulate the mechanical properties of natural vessels, 10 and albumen/SA hydrogel also has some elasticity and biocompatibility, especially in inducing vascularization within engineered tissues. 30 Therefore, In the present study, we demonstrate a novel approach combining FDM, dip-coating, and electrospinning techniques that overcomes these limitations, enabling the fabrication of biocompatible scaffolds with the three-layer structure of natural blood vessels and the tissue arrangement of HUVECs. The forming characteristics of FDM itself leave the fiber path direction of printing outside the print solid.…”

Section: Introductionmentioning

confidence: 99%

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An integrated strategy for designing and fabricating triple-layer vascular graft with oriented microgrooves to promote endothelialization

et al. 2021

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Compared with native blood vessels and existing vascular grafts, design and manufacture of vascular grafts with a three-dimensional topological structure is a key to induce cells and tissue growth, which remains an essential issue in both tissue engineering and regenerative medicine. This study sought to develop a novel triple-layer vascular graft (TLVG) with oriented microgrooves to investigate the mechanical property and endothelialization. The TLVGs were composed of electrospun Poly-ε-caprolactone (PCL)/thermoplastic polyurethane (TPU) as the inner layer, albumen/sodium alginate (SA) hydrogel as the middle layer, and electrospun PCL/TPU as the outer layer. In detail, a cylindrical sacrificial template was designed and printed using polyvinyl alcohol (PVA), served as the electrospinning receiving platform to form the oriented microgrooves in the inner layer of TLVGs. The highly elastic albumen/SA hydrogel and PCL/TPU nanofibers were able to simulate the elastin in blood vessels. In addition, the introduction of the albumen/SA hydrogel layer not only solves the leakage problem of a porous vascular graft but also improves the wettability of the scaffolds. The physicochemical properties and biological characteristics of TLVGs were evaluated by tensile testing, Surface wettability test, Fourier transform-infrared spectroscopy (FTIR) measurement, Live-Dead cell staining assay, and CCK-8 assay. Especially, the oriented microgrooves on the inner surface of the TLVGs can promote human umbilical vein endothelial cells (HUVECs) directed growth and migration in favor of endothelialization. All results showed that the fabricated TLVGs with excellent physicochemical properties and biocompatibility has great potential in clinic application.

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“…Hydrogel solutions were prepared at room temperature by mixing 5% (w/v) SA and albumen with 5:1 volume ratio. 30…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An integrated strategy for designing and fabricating triple-layer vascular graft with oriented microgrooves to promote endothelialization

et al. 2021

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“…Experiments showed that human umbilical vein endothelial cells not only maintained high cell viability on the composite scaffold, but also helped stimulate the germination of endothelial cells and the formation of vascular networks. Therefore, egg white‐based 3D composite bioink with good biological function has great potential in tissue and organ engineering 67 …”

Section: Application To Biomaterialsmentioning

confidence: 99%

“…Therefore, egg white-based 3D composite bioink with good biological function has great potential in tissue and organ engineering. 67 As a natural antibiotic, this bio-nanocomposite hydrogel can keep infected and non-infected wound surfaces moist, accelerate wound healing, and has no biological toxicity. Based on egg white, a bionic hybrid hydrogel prepared by simple UV polymerization method is used for bone regeneration.…”

Section: D Cell Culture Systemmentioning

confidence: 99%

An insight on egg white: From most common functional food to biomaterial application

Dong

Zhang

2020

J Biomed Mater Res

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Natural egg white tis widely used as an ingredient in nutritional foods and for food processing. Due to its characteristic foaming, emulsification, adhesion, and gelation, and its heat setting, biocompatibility, and low cost, research into the application and development of egg white in biomaterials, especially medical biomaterials, have been receiving attention. The composition and characteristics of egg white protein, and the physical mixing and chemically cross‐linking of egg white with other materials used to make degradable packaging films, bioceramics, bioplastics, biomimetic films, hydrogels, 3D scaffolds, bone regeneration, biopatterning, biosensors, and so forth, are reviewed in detail in this report. The novel egg white‐based biomaterials in various forms and applications could be constructed mostly through physical treatments such as ultrasonic wave, ultraviolet, laser and other radiation or high‐temperature calcination. Furthermore, the application and prospects for the use of egg white in biomaterials is also discussed.

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“…On the other hand, the use of 3D printing tech-nology in TE will not only solve the existing difficulties, but also may lead to significant progress in the field of regenerative medicine, due to some reasons such as the simplicity of 3D printing technology; the ability to create objects with a unique individual geometric shape; precision manufacturing of parts; the possibility of using various types of materials [8][9][10]. Today, the use of 3D printing in the field of medicine has a general name -bioprinting and includes the manufacture of implants [11] and biological scaffolds for growing organs and tissues [12], testing drugs [13], as well as direct printing of organs [14]. Unlike traditional methods used in 3D printing technology, materials in bioprinting are mainly presented by cells, biological materials and nutrients that stimulate cell growth.…”

Section: Introductionmentioning

confidence: 99%

A Numerical Study of Fluid Flow in the Porous Structure of Biological Scaffolds

Daulbayev

Mansurov

Sultanov

et al. 2020

Eurasian Chem. Tech. J.

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Tissue engineering (TE) is one of the promising areas that aims to address the global problem of organ and tissue shortages. The successful development of TE, particularly in bone tissue engineering, consists of the use of modern methods that allow the creation of scaffolds, the physicochemical, mechanical, and structural parameters of which will allow achieving the desired clinical results. The vast possibilities of the rapidly developing technology of three-dimensional (3D) printing, which allows the creation of individual scaffolds with high precision, has led to various developments in bone tissue TE. In this work, for the successful use of three-dimensional printing in TE to ensure the diffusion of nutrients during cell cultivation throughout the entire structure of the scaffold, a model of a rotating scaffold is proposed, and the movement of the diffusion flow of nutrient fluid is calculated based on Darcy’s law, which regulates the flow of fluids through porous media. The conducted studies of the rate of diffusion flow of nutrients based on glucose in the porous structure of scaffolds with a 10% content of calcium hydroxyapatite demonstrated the promise of using a model of a rotating composite scaffold in TE of bone tissue. The results show that at a scaffold rotation speed of 12 rpm, the diffusion flow rate of nutrients in the composite scaffolds porous structure is practically not affected by their geometric shape.

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Designing vascular supportive albumen-rich composite bioink for organ 3D printing

Cited by 40 publications

References 44 publications

An integrated strategy for designing and fabricating triple-layer vascular graft with oriented microgrooves to promote endothelialization

An integrated strategy for designing and fabricating triple-layer vascular graft with oriented microgrooves to promote endothelialization

An insight on egg white: From most common functional food to biomaterial application

A Numerical Study of Fluid Flow in the Porous Structure of Biological Scaffolds

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