Designing strong inducible synthetic promoters in yeasts
Jun Ishii,
Masahiro Tominaga,
Yoko Shima
et al.
Abstract:Inducible promoters are essential for precise control of target gene expression in synthetic biological systems. However, engineering eukaryotic promoters is often more challenging than engineering prokaryotic promoters due to their greater mechanistic complexity. In this study, we describe a simple and reliable approach for constructing strongly inducible synthetic promoters with minimum leakiness in yeast. The results indicated that the leakiness of yeast-inducible synthetic promoters is primarily the result… Show more
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