Designing protein nano-construct in ionic liquid: a boost in efficacy of cytochrome <i>C</i> under stresses

T., Sarath Kumar; Shet, Sachin M.; Bisht, Meena; Bharadwaj, Pranav; Pereira, Matheus M.; Franklin, Gregory; Nataraj, Sanna Kotrappanavar; Mondal, Dibyendu

doi:10.1039/d3cc00644a

Cited by 2 publications

(1 citation statement)

References 23 publications

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“…Although not considered in this review, the use of ILs to allow the stable immobilization of enzymes on nanomaterials is an emerging field. − Enzyme immobilization on nanoscale materials offers valuable advantages in enhancing enzyme stability during challenging reaction conditions. However, current immobilization methods suffer from a significant drawback: irreversible damage caused by the intense interaction between the enzyme and the carrier.…”

Section: Conclusion and Future Prospectsmentioning

confidence: 99%

Do Ionic Liquids Exhibit the Required Characteristics to Dissolve, Extract, Stabilize, and Purify Proteins? Past-Present-Future Assessment

Bharmoria,

Tietze,

Mondal

et al. 2024

Chem. Rev.

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Section: Conclusion and Future Prospectsmentioning

confidence: 99%

Do Ionic Liquids Exhibit the Required Characteristics to Dissolve, Extract, Stabilize, and Purify Proteins? Past-Present-Future Assessment

Bharmoria,

Tietze,

Mondal

et al. 2024

Chem. Rev.

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Understanding the Effect of Ionic Liquid–Mediated Solvent Engineering on the Kinetics and Thermodynamic Stability of Phenylalanine Ammonia-Lyase

Bharadwaj,

Barua,

Bisht

et al. 2024

J. Phys. Chem. B

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Phenylalanine ammonia-lyase (PAL) plays a central role in the phenylpropanoid pathway and in the treatment of phenylketonuria. However, the integration of PAL into sustainable industrial biocatalysis is hampered by its instability under harsh conditions. This study demonstrates that ionic liquid (IL)–assisted solvent (Tris-HCl buffer) engineering enables improvement of the reaction kinetics and thermodynamic stability of Rhodotorula glutinisPAL (RgPAL) under various stresses. Under optimized conditions, a 66.2% higher Kcat value, >60% remaining activity after 5 weeks of storage at room temperature, and >80% activity of RgPAL after incubation at 60 °C for 1 h were obtained in the [Ch][Ac]-blended Tris-HCl solvent compared to pristine Tris-HCl. The spectroscopic and molecular docking results suggest that the higher extent of hydration and the soft interactions complemented by the ILs with the D-chain residues of RgPAL jointly contributed to achieving more stable and active conformations of RgPAL. The enzyme showed a higher melting temperature (T m) in ILs+Tris-HCl compared to that in pristine Tris-HCl, with less change in enthalpy (ΔH fu) and entropy (ΔS fu) of unfolding. Overall, IL-mediated solvent engineering alters the microenvironment of RgPAL and allows the development of a robust PAL-based biocatalytic system.

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Designing protein nano-construct in ionic liquid: a boost in efficacy of cytochrome C under stresses

Abstract: Herein, we present a simple approach to fabricate protein nanoconstructs by complexing cytochrome C (Cyt C) with silk nanofibrils (SNF) and choline dihydrogen phosphate ionic liquid (IL). Molecular docking revealed...

Cited by 2 publications

References 23 publications

Do Ionic Liquids Exhibit the Required Characteristics to Dissolve, Extract, Stabilize, and Purify Proteins? Past-Present-Future Assessment

Do Ionic Liquids Exhibit the Required Characteristics to Dissolve, Extract, Stabilize, and Purify Proteins? Past-Present-Future Assessment

Understanding the Effect of Ionic Liquid–Mediated Solvent Engineering on the Kinetics and Thermodynamic Stability of Phenylalanine Ammonia-Lyase

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