Designing Olfactory Ensheathing Cell Transplantation Therapies: Influence of Cell Microenvironment

Murtaza, Mariyam; Mohanty, Lipsa; Ekberg, Jenny; John, James St

doi:10.1177/09636897221125685

Cited by 6 publications

(4 citation statements)

References 156 publications

(127 reference statements)

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“…Pharmaceutical agents targeting RhoA/ROCK and other signaling pathways have also shown potential [ 105 , 106 ]. Additionally, cell transplantation strategies offer promising avenues to modulate the glial scar environment [ 107 , 108 , 109 , 110 ].…”

Section: Current Challenges In the Treatment Of Scismentioning

confidence: 99%

The Promising Role of a Zebrafish Model Employed in Neural Regeneration Following a Spinal Cord Injury

Zeng,

Tsai

2023

IJMS

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Spinal cord injury (SCI) is a devastating event that results in a wide range of physical impairments and disabilities. Despite the advances in our understanding of the biological response to injured tissue, no effective treatments are available for SCIs at present. Some studies have addressed this issue by exploring the potential of cell transplantation therapy. However, because of the abnormal microenvironment in injured tissue, the survival rate of transplanted cells is often low, thus limiting the efficacy of such treatments. Many studies have attempted to overcome these obstacles using a variety of cell types and animal models. Recent studies have shown the utility of zebrafish as a model of neural regeneration following SCIs, including the proliferation and migration of various cell types and the involvement of various progenitor cells. In this review, we discuss some of the current challenges in SCI research, including the accurate identification of cell types involved in neural regeneration, the adverse microenvironment created by SCIs, attenuated immune responses that inhibit nerve regeneration, and glial scar formation that prevents axonal regeneration. More in-depth studies are needed to fully understand the neural regeneration mechanisms, proteins, and signaling pathways involved in the complex interactions between the SCI microenvironment and transplanted cells in non-mammals, particularly in the zebrafish model, which could, in turn, lead to new therapeutic approaches to treat SCIs in humans and other mammals.

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Section: Current Challenges In the Treatment Of Scismentioning

confidence: 99%

The Promising Role of a Zebrafish Model Employed in Neural Regeneration Following a Spinal Cord Injury

Zeng,

Tsai

2023

IJMS

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“…However, a common consequence in these studies is the poor survival of transplanted cells, with survival rates ranging from 0.3 percent to 3 percent. This issue is probably related to the fact that when OECs are isolated, expanded in vitro, and then transplanted into the injury site, their therapeutic potential is reduced, probably due to bleeding, damaged tissue and anatomical structures and hostile microenvironment present in the lesioned area (115)(116)(117). Therefore, in vitro models challenging OECs, by mimicking the injured tissue microenvironment, are needed.…”

Section: Oecs For Tissue Regeneration and Transplantationmentioning

confidence: 99%

“…For example, it has been found that preconditioned OECs showed increased migratory, phagocytic and immunomodulatory capacities. To improve their efficacy and yield upon graft, cells could be then exposed to a low oxygen level, or they could grow into three-dimensional scaffolds before being transplanted into the lesion ( 116 ).…”

Section: Oecs For Tissue Regeneration and Transplantationmentioning

confidence: 99%

Neurotrophic and immunomodulatory effects of olfactory ensheathing cells as a strategy for neuroprotection and regeneration

et al. 2022

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Accumulating evidence sustains glial cells as critical players during central nervous system (CNS) development, homeostasis and disease. Olfactory ensheathing cells (OECs), a type of specialized glia cells sharing properties with both Schwann cells and astrocytes, are of critical importance in physiological condition during olfactory system development, supporting its regenerative potential throughout the adult life. These characteristics prompted research in the field of cell-based therapy to test OEC grafts in damaged CNS. Neuroprotective mechanisms exerted by OEC grafts are not limited to axonal regeneration and cell differentiation. Indeed, OEC immunomodulatory properties and their phagocytic potential encourage OEC-based approaches for tissue regeneration in case of CNS injury. Herein we reviewed recent advances on the immune role of OECs, their ability to modulate CNS microenvironment via bystander effects and the potential of OECs as a cell-based strategy for tissue regeneration.

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“…Cell transplantation is one of the most promising strategies for the repair of central nervous system (CNS) injuries. Many studies have shown that transplantation of olfactory ensheathing cells (OECs) can induce axon regeneration, remyelination, and restoration of function in experimental spinal cord injuries 1 – 12 . Our previous studies showed that transplantation of cultured bulbar OECs in rats to bridge transected dorsal roots to the spinal cords induced the regeneration of axons and restored directed forepaw reaching and vertical climbing functions 6 , 13 .…”

Section: Introductionmentioning

confidence: 99%

Transplantation of Cryopreserved Olfactory Ensheathing Cells Restores Loss of Functions in an Experimental Model

Minkelyte,

Li,

et al. 2023

Cell Transplant

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In the past decades, the properties of olfactory ensheathing cells (OECs) have been widely investigated. Studies have shown that transplantation of OECs cultured from the olfactory bulb mediates axonal regeneration, remyelination and restores lost functions in experimental central nervous system (CNS) injury models. Autologously sourcing the cells from the nasal mucosa or the olfactory bulb to treat patients with spinal cord injuries would be ideal, but the cell yield achieved may be inadequate to cover the surface area of the lesions typically encountered in human spinal cord contusion injuries. Therefore, banking allogenic cryopreserved olfactory bulb cells from donors or generating cell lines could provide a marked increase in cell stock available for transplantation. This study is undertaken in two control and two intervention groups. The control groups have lesions alone and lesions with collagen gel but without cells. The intervention groups have either transplantation of primary cultured olfactory bulb OECs (bOECs) encapsulated in collagen gel or cryopreserved bulb OECs (CbOECs) encapsulated in collagen gel. Here, we report that transplantation of cryopreserved rat bOECs encapsulated in collagen restored the loss of function in a vertical climbing test in a unilateral C6-T1 dorsal root injury model. The loss of function returns in 80% of rats with injuries in about 3 weeks comparable to that we observed after transplantation of primary cultured bOECs. The regeneration axons induced by the transplant are identified by neurofilament antibodies and ensheathed by OECs. Our results indicate that cryopreserved OECs retain their properties of inducing axon regeneration and restoring loss of function in the experimental model. This is a step forward to translate the research into future clinical applications.

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Designing Olfactory Ensheathing Cell Transplantation Therapies: Influence of Cell Microenvironment

Cited by 6 publications

References 156 publications

The Promising Role of a Zebrafish Model Employed in Neural Regeneration Following a Spinal Cord Injury

The Promising Role of a Zebrafish Model Employed in Neural Regeneration Following a Spinal Cord Injury

Neurotrophic and immunomodulatory effects of olfactory ensheathing cells as a strategy for neuroprotection and regeneration

Transplantation of Cryopreserved Olfactory Ensheathing Cells Restores Loss of Functions in an Experimental Model

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