Designing of New Optical Immunosensors Based on 2-Amino-4-(anthracen-9-yl)-7-hydroxy-4H-chromene-3-carbonitrile for the Detection of Aeromonas hydrophila in the Organs of Oreochromis mossambicus Fingerlings

Abstract

A one-pot greener methodology has been adopted for the synthesis of a simple 4 H -chromene core-based fluorescent tag of ( S )-2-amino-4-(anthracen-9-yl)-7-hydroxy-4 H -chromene-3-carbonitrile (AHC), and its structure has been analyzed using NMR spectroscopy. The physicochemical properties of AHC were well-studied by UV–vis and fluorescent spectroscopy techniques. As a result of excellent emitting property (ϕ ≈ 0.75), it has been coupled with anti-AH through amide linkage, and the AHC-tagged anti-AH has been used as an immunoassay for the selective detection of Aeromonas hydrophila in the presence of interfering pathogens. Under optimized conditions, immunosensors could successfully quantify A. hydrophila from 4 to 736 CFU/mL, and the LOD was 2 CFU/mL. Saliently, the immunoassay has been successfully demonstrated for the analysis of A. hydrophila in the organs of Oreochromis mossambicus fingerlings, and results have shown a very good agreement with our optimized neat AH fluorimetric titration results.

Phenotypic, Molecular Detection And Antibiogram Analysis of Aeromonas Hydrophila from Oreochromis Niloticus (Nile Tilapia) And Ready-To- Eat Fish Products in selected Rift Valley Lakes of Ethiopia

“…A lower percentage (2.3%) of A. hydrophila was reported in RTE sh product in India by Gupta. Whereas, a higher percentage (77.3%) in RTE fried sh in India was also reported by (40) Manna et. al (2013).????…”

Nanotechnology‐based electrochemical biosensors for monitoring breast cancer biomarkers

“…Abs possess -NH 2 and -COOH functional in their structures. [112][113][114][115] A covalent binding between the Ab molecule and platform components is required to attach the Ab to the platform with high strength and stability. In unmodified Abs, forming amide bonds is the best option for this purpose.…”

“…In unmodified Abs, forming amide bonds is the best option for this purpose. 115,116 The use of specific platforms and reagents are options for immobilizing Ab molecules on the working electrode. In confronting carboxylated platforms (such as reduced GO, rGO), the NH residual, and for amine-containing ones (such as chitosan, CS), the -COOH end is beneficial to share in amide bond formation.…”

The Immobilized Zirconocene Chloride on Magnetite‐reduced Graphene Oxide: A Highly Efficient and Reusable Heterogeneous Nanocatalyst for One‐pot Three‐component Synthesis of Tetrahydrobenzo[b]pyrans and Dihydropyrano[3,2‐c]chromenes

“…[38] These compounds are also found in vegetables and fruits [39] and they make a part of structure in alkaloids and anthocyanin. [40] The 81 m 2 g À 1 678.1 m 2 g À 1 48.95 m 2 g À 1 0.21 cm 3 g À 1 5.3 nm ChemistrySelect literature review shows that derivatives of 2-amino-4H-chromene-3-carbonitrile have been used in compounds with antimicrobial activity, [41] sex hormone, [42] antitumor, [43] antiviral, [44] mutagenic, [45] antiproliferative, cancer therapy [46,47] and central nervous system drugs. These materials have a great importance in the treatment of neurodegenerative diseases like Parkinson's disease, Down's syndrome, Alzheimer's disease, AIDS associated dementia [48] and treatment of schizophrenia.…”

“…2‐Amino‐4 H ‐chromene‐3‐carbonitriles are oxygen containing heterocyclic compounds which are major skeleton of many agrochemicals and natural product scaffolds, and widely used in cosmetic and pigment materials [38] . These compounds are also found in vegetables and fruits [39] and they make a part of structure in alkaloids and anthocyanin [40] . The literature review shows that derivatives of 2‐amino‐4 H ‐chromene‐3‐carbonitrile have been used in compounds with antimicrobial activity, [41] sex hormone, [42] antitumor, [43] antiviral, [44] mutagenic, [45] antiproliferative, cancer therapy [46,47] and central nervous system drugs.…”