Designing of Fermenter and its utilization in food industries

Gaikwad,; Panghal, Anil; Jadhav, Swapnaja K.; Sharma, Prateek; Bagal, A; Jadhav, Ankit; Chhikara, Navnidhi

doi:10.20944/preprints201808.0433.v1

Cited by 10 publications

(9 citation statements)

References 3 publications

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“…The experiment aimed to obtain cell and product quantities at a large scale (the industrial or pilot-plant scale) with at least the same efficiency as that obtained at a laboratory scale. However, upscaling is not an easy task because different reduction foam efficiencies, substrate bioavailability levels, oxygen transfer efficiencies and adverse physical or biological effects can occur at different fermentation scales 50 .…”

Section: Discussionmentioning

confidence: 99%

Large scale production of indole-3-acetic acid and evaluation of the inhibitory effect of indole-3-acetic acid on weed growth

Bunsangiam

Thongpae

Limtong

et al. 2021

Sci Rep

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Indole-3-acetic acid (IAA) is the most common plant hormone of the auxin class and regulates various plant growth processes. The present study investigated IAA production by the basidiomycetous yeast Rhodosporidiobolus fluvialis DMKU-CP293 using the one-factor-at-a-time (OFAT) method and response surface methodology (RSM). IAA production was optimized in shake-flask culture using a cost-effective medium containing 4.5% crude glycerol, 2% CSL and 0.55% feed-grade l-tryptophan. The optimized medium resulted in a 3.3-fold improvement in IAA production and a 3.6-fold reduction in cost compared with those obtained with a non-optimized medium. Production was then scaled up to a 15-L bioreactor and to a pilot-scale (100-L) bioreactor based on the constant impeller tip speed (Vtip) strategy. By doing so, IAA was successfully produced at a concentration of 3569.32 mg/L at the pilot scale. To the best of our knowledge, this is the first report of pilot-scale IAA production by microorganisms. In addition, we evaluated the effect of crude IAA on weed growth. The results showed that weed (Cyperus rotundus L.) growth could be inhibited by 50 mg/L of crude IAA. IAA therefore has the potential to be developed as a herbicidal bioproduct to replace the chemical herbicides that have been banned in various countries, including Thailand.

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Section: Discussionmentioning

confidence: 99%

Large scale production of indole-3-acetic acid and evaluation of the inhibitory effect of indole-3-acetic acid on weed growth

Bunsangiam

Thongpae

Limtong

et al. 2021

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The experiment aimed to obtain cell and product quantities at a large scale (the industrial or pilot-plant scale) with at least the same e ciency as that obtained at a laboratory scale. However, upscaling is not an easy task because different reduction foam e ciencies, substrate bioavailability levels, oxygen transfer e ciencies and adverse physical or biological effects can occur at different fermentation scales 49 .…”

Section: Discussionmentioning

confidence: 99%

Production of Indole-3-Acetic Acid: A White Biotechnology for Weed Biocontrol

Bunsangiam

Thongpae

Limtong

et al. 2021

Preprint

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Indole-3-acetic acid (IAA) is the most common plant hormone of the auxin class and regulates various plant growth processes. The present study investigated IAA production by the basidiomycetous yeast Rhodosporidiobolus fluvialis DMKU-CP293 using the one-factor-at-a-time (OFAT) method and response surface methodology (RSM). IAA production was optimized in shake-flask culture using a cost-effective medium containing 4.5% crude glycerol, 2% CSL and 0.55% feed-grade L-tryptophan. The optimized medium resulted in a 3.3-fold improvement in IAA production and a 3.6-fold reduction in cost compared with those obtained with a non-optimized medium. Production was then scaled up to a 15-L bioreactor and to a pilot-scale (100-L) bioreactor based on the constant impeller tip speed (Vtip) strategy. By doing so, IAA was successfully produced at a concentration of 3,569.32 mg/L at the pilot scale. To the best of our knowledge, this is the first report of pilot-scale IAA production by microorganisms. In addition, we evaluated the effect of crude IAA on weed growth. The results showed that weed (Cyperus rotundus L.) growth could be inhibited by 50 mg/L of crude IAA. IAA therefore has the potential to be developed as a herbicidal bioproduct to replace the chemical herbicides that have been banned in various countries, including Thailand.

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“…A functional ALSB (50 cm in height and 15 cm in diameter), as illustrated in Figure 1A, was constructed. Numerous modifications were made to the bioreactor for a variety of purposes for different colony forming fungi or mycelia [16].…”

Section: Air-l-shaped Bioreactor (Aslb) Designmentioning

confidence: 99%

Performance of Biomass and Exopolysaccharide Production from the Medicinal Mushroom Ganoderma lucidum in a New Fabricated Air-L-Shaped Bioreactor (ALSB)

et al. 2023

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Conventional stirred-tank bioreactor (STR) designs are optimised for cultures of bacteria but not fungal cultures; therefore, a new Air-L-Shaped Bioreactor (ALSB) was fabricated. The ALSB was designed to eliminate the wall growth and clumping of fungal mycelium in STRs. Ganoderma lucidum was used as a fungal model and its biomass and exopolysaccharide (EPS) production were maximised by optimising the agitation rate, glucose concentration, initial pH, and aeration via response surface methodology (RSM). The ALSB system generated 7.8 g/L of biomass (biomass optimised conditions: 110 rpm, 24 g/L glucose, pH 5.6, and 3 v/v of aeration) and 4.4 g/L of EPS (EPS optimised conditions: 90 rpm, 30 g/L glucose, pH 4, and 2.5 v/v of aeration). In combination, for both optimised conditions, biomass (7.9 g/L) and EPS (4.6 g/L) were produced at 110 rpm, 30 g/L glucose, pH 4, and 3 v/v of aeration with minimal wall growth. The data prove that the ALSB is a blueprint for efficient economical fungal cultivation.

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Designing of Fermenter and its utilization in food industries

Cited by 10 publications

References 3 publications

Large scale production of indole-3-acetic acid and evaluation of the inhibitory effect of indole-3-acetic acid on weed growth

Large scale production of indole-3-acetic acid and evaluation of the inhibitory effect of indole-3-acetic acid on weed growth

Production of Indole-3-Acetic Acid: A White Biotechnology for Weed Biocontrol

Performance of Biomass and Exopolysaccharide Production from the Medicinal Mushroom Ganoderma lucidum in a New Fabricated Air-L-Shaped Bioreactor (ALSB)

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