Designing monographs for Rosmarinus officinalis L. and Lavandula angustifolia L.: Two Lebanese species with significant medicinal potentials

Abstract

Introduction:The world health organization recommends validating medicinal plants to ensure safety and efficacy. Lavandula is valued for the production of essential oil used in aromatherapy. Rosmarinus is known for its folk use as anti-oxidant and antidiabetic. This study aims to design monographs for Rosmarinus officinalis (Linn.) and Lavandula angustifolia (Linn.) cultivated, in conditions similar to their natural habitat, at Beirut Arab University botanical garden. Also screening the antioxidant activity of both plants, and assessing the antidiabetic effect of R. officinalis (Linn.). Methods: macroscopic and microscopic examination, hydrodistillation to obtain essential oils (EOs), thin layer chromatography, Gas chromatography-Mass spectrometry, and physiochemical properties of the EOs were determined. The antioxidant effect of the EOs is determined by DPPH assay. The antidiabetic effect of Rosemary EO is investigated using alloxan-induced diabetic mice. Results: the qualities of studied species are in agreement with the standards described in WHO monographs and in the British pharmacopeia. Composition of the EO produced from either Rosemary (REO) or Lavender (LEO) collected at different months showed differences. The EO of both showed significant antioxidant effects in DPPH assay (p<0.05). In vivo investigation of the antidiabetic effect of REO showed a significant antidiabetic effect in diabetic mouse model (p<0.05). Conclusion: both plant species and their EOs fulfilled most of standards specified by the British Pharmacopeia 2013. Rosemary collected in April (Spring) showed the best antioxidant activity. Lavender collected in September (Early Fall) showed the best antioxidant activity. REO revealed significant lowering in blood glucose level at the dose of 110 mg/kg.

Determining Leaf Yield, Some Plant Characters and Leaf Essential Oil Components of Different Cultivars of Lavender and Lavandin (Lavandula Spp.) on the Harran Plain Ecological Conditions

“…Some similarities were recorded between our study and the previous reports. Investigation of our results revealed that the main component of leaves essential oils of lavender and lavandin cultivars were found 1.8 Cineol, like other researchers (Hassanpouraghdam et al, 2011;Meftahizade et al, 2011;Touati et al, 2011;Koleilat et al, 2017). Baydar (2009) stated that better quality essential oil was included low camphor content and the camphor content in quality lavender oil must be between 0.5 and 1% in lavenders and between 5.0 and 10.0 in lavandins.…”

“…Essential oils of Lavandula species have been previously investigated by some researchers. In lavender, it was found the main components of the essential oil that in leaves 1,8-cineole 25.70% and borneol 11.32% (Meftahizade et al, 2011) In lavandin, it was found the main component of the inflorescence essential oil that 1,8-cineole 33.54% and camphor 18.89% (Koleilat et al, 2017), linalool 34.8-43.3%, linalyl acetate 3.76-44.46% and camphor 5.28-19.8% (Kara and Baydar, 2013). In L. dentata, it was found the main component of the leaf essential oil that 1,8-cineole 33.54% and camphor 18.89% (Touati et al, 2011).…”

Aromaterapia de Rosmarinus officinalis L. (Lamiaceae) em testes in vivo: revisão sistemática

“…Outro ponto representado foi à forma de aplicação dos OEs na aromaterapia, observando-se que nos artigos analisados oito trabalhos (61,54%) focaram seus testes no método de massagem aromática e 38,46% (cinco artigos) realizaram analises por meio da inalação dos OEs Os OEs são compostos voláteis oriundos de diferentes partes na planta. Como A R. officinalis é uma planta que possui tanto as folhas quantos as flores aromáticas devido à presença de compostos voláteis presentes nos tricomas glandulares (Koleilat, Raafat, El-Lakany, & Aboul-Ela, 2017), os principais compostos químicos encontrado no OE desta espécie são α-pineno, cânfora e 1,8-cineol (Sayorwan et al, 2013). O OE ao ser inalado apresenta propriedades estimulante, ansiolítica, diminui o fluxo sanguíneo e aumenta a pressão arterial sistólica (Farner, Reed, Abbas, Shmina, & Bielawski, 2019;Sayorwan et al, 2013).…”

Novel dual-function GC/MS aided ultrasound-assisted hydrodistillation for the valorization of Citrus sinensis by-products: phytochemical analysis and anti-bacterial activities

“…1 and S1 . The in-line split injector was used for separation and quantification of essential oils compounds 14 . The Waters GC–FID–MS was operated utilizing 5-MS capillary column (30 m * 0.25 mm * 0.25 μm) in addition to a Waters flame ionization detector (FID), and a mass detector operated in EI mode.…”