Designing enzyme cocktails from Penicillium and Aspergillus species for the enhanced saccharification of agro-industrial wastes

Karp, Susan Grace; Рожкова, А. М.; Семенова, М. В.; Осипов, Д. О.; Pauli, Suellen Teixeira Zavadzki de; Синицына, О. А.; Зоров, И. Н.; Vandenberghe, Luciana Porto de Souza; Soccol, Carlos Ricardo; Sinitsyn, A. P.

doi:10.1016/j.biortech.2021.124888

Cited by 18 publications

(3 citation statements)

References 29 publications

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“…Создание штаммов -продуцентов различных ферментов, предназначенных для биоконверсии ВРС, существенно облегчило процедуру их наработки в гомогенном виде в препаративном количестве. Это позволило провести цикл исследований, в которых различные мультиферментные композиции формировались на базе гомогенных ферментов, что создало возможность тщательной юстировки состава мультиэнзимных композиций, адаптированных по качественному и количественному составу для достижения максимальной эффективности процесса биоконверсии различных видов ВРС [141,142]). Например, было показано, что оптимальный ферментный комплекс для гидролиза МКЦ состоит из ЦБГIа, ЦБГIв, ЦБГII, ЭГII, ЭГV, БГЛ и ксиланазы в соотношении 1:1:2:0,3:0,4:0,2:0,1; оптимальный комплекс для гидролиза предобработанной органозольвом древесины пихты состоит из ЦБГIа,, ЦБГII, ЭГII и БГЛ в соотношении 2:2:0,8:0,2 [130].…”

Section: создание продуцентов целлюлаз и других карбогидраз генно-инж...unclassified

“…Например, было показано, что оптимальный ферментный комплекс для гидролиза МКЦ состоит из ЦБГIа, ЦБГIв, ЦБГII, ЭГII, ЭГV, БГЛ и ксиланазы в соотношении 1:1:2:0,3:0,4:0,2:0,1; оптимальный комплекс для гидролиза предобработанной органозольвом древесины пихты состоит из ЦБГIа,, ЦБГII, ЭГII и БГЛ в соотношении 2:2:0,8:0,2 [130]. Ферментный комплекс, позволивший осуществить практически 100%-ю конверсию свекловичного жома содержит ЦБГ, ЭГ, БГ, арабиноксилан-арабинофурангидролазу, пектинлиазу, полигалактуроназу (основные ферменты), а также эндо-или экзо-арабиназы, эндогалактаназу, β-ксилозидазу, эндо-ксиланазу и/ или α-арабинофуранозидазу (вспомогательные ферменты) [141]. Далее осуществляется воспроизводство оптимального соотношения ферментов комплекса, но уже с использованием ФП, получение которых реализуется за счет создания с помощью генно-инженерных методов соответствующих рекомбинантных штаммов-продуцентов.…”

Section: создание продуцентов целлюлаз и других карбогидраз генно-инж...unclassified

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Carbohydrases – 50 Years of Research at the Department Of Chemical Enzymology of Lomonosov Moscow State University, History and Prospects

Sinitsyn,

Sinitsyna,

Zorov

et al. 2023

Lomonosov Chemistry Journal

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The review describes the history of the development of research on carbohydrasеs conducted at the Department of Chemical Enzymology from the mid-1970s to the present. The results concerning the study of the mechanism and kinetics of the processes of enzymatic conversion of cellulose and renewable plant raw materials under the action of a multi-enzyme cellulases complexes; the role of individual components of these complexes - basic (endoglucanases and cellobiohydrolases) and auxiliary enzymes (polysaccharide monooxygenase, β-glucosidase, xylanase) and their synergistic interaction. The features of using reactors of various designs for bioconversion of plant raw materials are described: periodic type, continuous column type, reactor for hydrolysis in a constant electric field, reactor with intensive mixing by ferromagnetic particles in magnetic field. The possibilities of increasing the reactivity of plant raw materials using various pretreatment methods, as well as the influence of the structural and physico-chemical properties of cellulose on the efficiency of its enzymatic conversion are discussed. Data on the creation of highly active strains of microscopic fungi-producers of cellulases and other carbohydrases using methods of induced mutagenesis - Trichoderma (Hypocrea), Penicillium (Talaromyces), Aspergillus, Chrysosporium (Myceliophtora) spp., as well as data on the composition of the enzyme complexes produced by them and the properties of the enzymes forming them are presented. It describes the creation of expression systems based on P. canescens and P. verruculosum and the production of recombinant producer strains with their help, which made it possible to obtain enzyme preparations (EP) that ensure high efficiency of bioconversion processes of plant raw materials, as well as to create producers of a wide range of carbohydrases for practical use in various fields of industry and agriculture. A number of industrially important EP obtained using the P. verruculosum expression system are currently being produced at the Agroferment LLC plant.

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Section: создание продуцентов целлюлаз и других карбогидраз генно-инж...unclassified

Carbohydrases – 50 Years of Research at the Department Of Chemical Enzymology of Lomonosov Moscow State University, History and Prospects

Sinitsyn,

Sinitsyna,

Zorov

et al. 2023

Lomonosov Chemistry Journal

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“…has the potential to be adopted in algal biorefinery processes for biofuel production because of its arsenal of degrading enzymes [2]. Enzymes from Penicillium species are also crucial for the enhanced saccharification of agroindustrial wastes, which can be used to build a bio-based economy [3]. In the medicinal field, the best-known antibiotic penicillin was produced by P. chrysogenum Thom [4].…”

Section: Introductionmentioning

confidence: 99%

Three New Species of Penicillium from East and Northeast China

Song,

Ding,

Zhuang

et al. 2024

JoF

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Penicillium species are ubiquitous in the environment and are of substantial importance, especially in industrial and medical aspects. During our investigation of the biodiversity of Penicillium, three new species were discovered in soil samples collected from East and Northeast China. They were determined as new to science based on morphological comparisons and phylogenetic analyses, and were found to belong to the subgenus Penicillium section Robsamsonia and subgenus Aspergilloides sections Aspergilloides and Citrina. Descriptions and illustrations of these species are provided, and their geographic distributions are also discussed.

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Thermostable Recombinant Cellulases of the Thermophilic Mold Myceliophthora thermophila in the Bioconversion of Paddy Straw and Sugarcane Bagasse to Ethanol

Dadwal,

Sharma,

Satyanarayana

2023

Bioenerg. Res.

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Designing enzyme cocktails from Penicillium and Aspergillus species for the enhanced saccharification of agro-industrial wastes

Cited by 18 publications

References 29 publications

Carbohydrases – 50 Years of Research at the Department Of Chemical Enzymology of Lomonosov Moscow State University, History and Prospects

Carbohydrases – 50 Years of Research at the Department Of Chemical Enzymology of Lomonosov Moscow State University, History and Prospects

Three New Species of Penicillium from East and Northeast China

Thermostable Recombinant Cellulases of the Thermophilic Mold Myceliophthora thermophila in the Bioconversion of Paddy Straw and Sugarcane Bagasse to Ethanol

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