Designing chitosan-dextran sulfate nanoparticles using charge ratios

Abstract

The purpose of this study was to examine the effect of charge ratio on the formation and properties of the chitosan (CS)-dextran sulfate (DS) nanoparticles developed for the delivery of water-soluble small and large molecules, including proteins. Rhodamine 6G (R6G) and bovine serum albumin (BSA) were chosen as model molecules. CS-DS nanoparticles were formulated by a complex coacervation process under mild conditions. The influence of formulation and process variables, including the charge ratio of the 2 ionic polymers, on particle size, zeta potential, and nanoparticle entrapment of R6G and BSA was studied. The in vitro release of R6G and BSA was also evaluated, and the integrity of BSA in the release fraction was assessed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Depending on the concentration and charge ratio of DS and CS, nanoparticles with varied size (>or=244 nm) and zeta potential (-47.1-60 mV) were obtained. High entrapment efficiency (98%) was achieved for both R6G and BSA when the charge ratio of the 2 ionic polymers was greater than 1.12. The release of both R6G and BSA from nanoparticles was based on the ion-exchange mechanism. BSA showed much slower continuous release for up to 7 days while still maintaining its integrity for an extended period. The CS-DS nanoparticles developed based on the modulation of charge ratio show promise as a system for controlled delivery of both small and large molecules, including proteins.

Chitosan Nanoparticles: A Promising System in Novel Drug Delivery

“…13,54,55) The weight ratio of the two polymers plays a major role in controlling particle size, surface charge, entrapment efficiency and release characteristics of the nanoparticles to be formulated. 56,57) The physicochemical and release characteristics of the chitosan (CS)-dextran sulfate (DS) nanoparticles can be varied by varying the ratios of two ionic polymers.…”

Peptide/protein vaccine delivery system based on PLGA particles

“…It is assumed that the burst release happens due to weakly bound or adsorbed proteins to particle surface. 54 It has been mentioned that the lag time relies on PLGA molecular weight (MW) and end-group caps. 52,53 Water absorption by PLGA polymer starts immediately upon exposure of PLGA particles to water, or in vivo administration.…”

Storage Stabilisation of Albumin-Loaded Chitosan Nanoparticles by Lyoprotectants

“…Among various materials investigated for preparing polymeric nanoparticles; chitosan (CS), a natural bio-polymer derived from chitin deacetylation [4,5] has attained considerable attention due to its excellent biocompatibility, biodegradability and antibacterial properties [6]. Recently, polymeric nanoparticles have been prepared from chitosan and dextran sulphate (DS) with higher stability and mechanical strength as compared to chitosantripolyphosphate (CS/TPP) nanoparticles [7,8]. However, their clinical applications are often limited by the thermodynamic instability of their colloidal dispersion for prolonged time periods.…”