Designing Blood-stage Vaccines against Babesia bovis and B. bigemina

Brown, Wendy C.; Palmer, Guy H.

doi:10.1016/s0169-4758(99)01471-4

Cited by 137 publications

(134 citation statements)

References 52 publications

Supporting

Mentioning

127

Contrasting

Unclassified

Order By: Relevance

“…Our data provide direct evidence that in the absence of T and B cells, infected C57BL/6 mice can mount a protective response against Babesia, which supports the view that innate mechanisms play a prominent role in immunity to babesiosis (6,16,30). Observations of BALB/c SCID mice, however, suggest that acquired immunity may still play an important role if the genetic context decreases the efficiency of innate immune responses.…”

supporting

confidence: 82%

Innate Resistance toBabesiaInfection Is Influenced by Genetic Background and Gender

et al. 2001

View full text Add to dashboard Cite

Infection of severe combined immunodeficient mice with Babesia sp. strain WA1 was studied to assess the contributions of innate and adaptive immunity in resistance to acute babesiosis. The scid mutation showed little effect in genetically susceptible C3H mice and did not decrease the inherent resistance of C57BL/6 mice to the infection, suggesting that innate immunity plays a central role in determining the course of Babesia infection in these strains. In contrast, the scid mutation dramatically impaired resistance in moderately susceptible BALB/c mice, suggesting that acquired immunity may play an important secondary role. In comparison to their female counterparts, male mice of different genetic backgrounds showed increased resistance to the infection, indicating that the gender of the host may influence protection against babesiosis.

show abstract

supporting

confidence: 82%

Innate Resistance toBabesiaInfection Is Influenced by Genetic Background and Gender

et al. 2001

View full text Add to dashboard Cite

show abstract

“…Bovine B. bovis-immune serum can immunoprecipitate the whole recombinant RAP-1 or a product of the RAP-1 deletion clone (25). Therefore, it was considered a candidate antigen for use in vaccine development (4,7,19) and to have potential as a diagnostic antigen for the detection of anti-B. bovis antibodies in cattle.…”

Section: Methodsmentioning

confidence: 99%

“…The major immunogenic B-cell and T-cell epitopes on RAP-1 are conserved among all strains tested, but they are not conserved between different species (5,24). The lack of extensive differences in RAP-1 among geographically distinct isolates of B. bovis suggests that RAP-1 should be considered a candidate antigen in the development of a diagnostic reagent and subunit vaccine (4,7,19). In this study, the gene encoding B. bovis RAP-1 was expressed in insect cells by using a baculovirus expression system.…”

mentioning

confidence: 99%

Evaluation of an Enzyme-Linked Immunosorbent Assay with Recombinant Rhoptry-Associated Protein 1 Antigen against Babesia bovis for the Detection of Specific Antibodies in Cattle

et al. 2002

View full text Add to dashboard Cite

The gene encoding Babesia bovis rhoptry-associated protein 1 (RAP-1) was used to develop an enzyme-linked immunosorbent assay (ELISA) to measure specific antibodies against B. bovis. The B. bovis RAP-1 gene was subcloned into a baculovirus transfer vector, and the RAP-1 protein was expressed in insect cells infected with a recombinant baculovirus. The recombinant B. bovis RAP-1 of 65 kDa was detected with anti-RAP-1 mouse serum by Western blotting, and this recombinant RAP-1 was used as an antigen in the ELISA. The ELISA was able to differentiate between B. bovis-infected sera and B. bigemina-infected sera or noninfected normal bovine sera. The results demonstrate that the recombinant RAP-1 expressed in insect cells might be a useful antigen for the detection of antibodies to B. bovis.

show abstract

“…and Anaplasma spp. [2,3]. In the present study, cellular reaction in animals immunized with recombinant MPSP were investigated.…”

Section: Discussionmentioning

confidence: 96%

Epitope-Mapping of Antigen-Specific T Lymphocyte in Cattle Immunized with Recombinant Major Piroplasm Surface Protein of Theileria sergenti.

Kakuda

Sugimoto

Onuma

2001

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. The cellular immune responses against major piroplasm surface protein (MPSP) of Theileria sergenti were characterized. Three cattle were immunized with recombinant MPSP (C type) encapsulated by mannan-coated liposome. The proliferative responses of peripheral blood mononuclear cells (PBMC) against MPSP were detected in all immunized animals. Reverse transcriptase polymerase chain reaction (RT-PCR) analysis revealed that T cell lines derived from each animal expressed relatively high levels of interferon (IFN)-γ mRNA, low levels of interleukin (IL)-2, IL-10, and tumor necrosis factor (TNF)-α mRNAs, but no detectable level of IL-4 mRNA. From the results of T cell epitope-mapping, T-cell lines from two animals responded to DTSKFTPTVAHRLKHAEDLF (residues 61 to 80), while other animal responded to GTGKVYDFVGNFKVTKVKFE (residues 141 to 160). The MPSP-type specific response of a Tcell line was observed in the latter region of MPSP. These data suggest that immunization with cocktail vaccine consisting of different types of MPSP may be necessary in the field trial. KEY WORDS: epitope-mapping, T-cell line, Theileria sergenti.

show abstract

Designing Blood-stage Vaccines against Babesia bovis and B. bigemina

Cited by 137 publications

References 52 publications

Innate Resistance toBabesiaInfection Is Influenced by Genetic Background and Gender

Innate Resistance toBabesiaInfection Is Influenced by Genetic Background and Gender

Evaluation of an Enzyme-Linked Immunosorbent Assay with Recombinant Rhoptry-Associated Protein 1 Antigen against Babesia bovis for the Detection of Specific Antibodies in Cattle

Epitope-Mapping of Antigen-Specific T Lymphocyte in Cattle Immunized with Recombinant Major Piroplasm Surface Protein of Theileria sergenti.

Contact Info

Product

Resources

About